Calnexin

Doxorubicin hydrochloride (Dox, MedChemExpress LLC, Shanghai, China). BCA protein assay Kit, 3,3′‐dioctadecyloxacarbocyanine perchlorate (DiO), 1,1′‐dioctadecyl‐3,3,3′,3′‐tetramethylindocarbocyanine perchlorateand (DiI), and 1,1′‐dioctadecyl‐3,3,3′,3′‐tetramethyl indotricarbocyanine iodide (DiR) were supplied by Beyotime (Shanghai, China). Lipofectamine 2000 transfection reagent was sourced from Invitrogen (USA). Matrigel was obtained from BD (USA). Creatine kinase MB isoenzyme and aspartate aminotransferase were purchased from Wuhan USCN Business Co. Ltd, China. LRP1, Alix, CD63, and CD9 antibodies were purchased from Abcam (USA), while HA, EGFP, Calnexin and β‐actin antibodies were purchased from Beyotime. All primers were synthesised by GENEWIZ (Suzhou, China). bEnd.3, U87MG, HEK293T, A549, SH‐SY5Y and HepG2 cell lines were purchased from Procell (Wuhan, China) and cultured in DMEM supplemented with 10% foetal bovine serum and 1% penicillin and streptomycin (Gibco, USA) under a 5% CO₂ atmosphere at 37 °C. BALB/c nude mice, aged 4–6 weeks, were provided by Cavens (Changzhou, China). All experiments related to animals were performed according to the guidelines estimated and approved by the Ethics Committee of the Suzhou Institute of Nano‐Tech and Nano‐Bionics, Chinese Academy of Sciences (Assigned approval number: SINANO/EC/2021‐065).