Intracellular Ca2+ measurement was performed as described47 (link). Briefly, cells were incubated in HBSS containing 4 mM Fluo-4 AM for 1 h, washed with HBSS three times and incubated at room temperature for another 10 min. Then, 200 nM ionomycin (Biogem) was applied extracellularly and MFI of Fluo-4 was recorded within 100 s by flow cytometry (NovoCyte 1050 system, Agilent).
Novocyte 1050 system
Manufactured by Agilent Technologies
The NovoCyte 1050 system is a flow cytometer designed for cell analysis. It offers multicolor detection, high-throughput sample processing, and user-friendly software. The NovoCyte 1050 system can be used to analyze a variety of cell types and samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using novocyte 1050 system
2
Intracellular pH Measurement by pHrodo
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Intracellular acidity was determined with pHrodo Red AM (Thermo Fisher). Cells were washed with PBS and labeled with pHrodo for 30 min. Cells were analyzed at 0–90 min after addition of 10 mM LA by flow cytometry (NovoCyte 1050 system, Agilent).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!