McCoy’s 5A medium and calf serum were obtained from Biological Industries, Israel; CCK-8 reagents were obtained from APExBIO, USA; qRT-PCR kits were obtained from Tiangen Biotech (Beijing, China) Co., Ltd.; PCR primers were synthesized in Sangon Biotech (Shanghai, China) Co., Ltd.; Western Blot reagents were obtained from Beyotime Biotechnology, China; rabbit anti-human monoclonal antibodies (first antibodies) were obtained from Affinity Biosciences, USA; rabbit anti-human GAPDH monoclonal antibodies (first antibodies) were obtained from Hangzhou Huabio, China; horseradish peroxidase (HRP)-labeled sheep anti-rabbit polyclonal antibodies were obtained from Sera Care Life Sciences, USA; cell apoptosis assay kits were obtained from Nanjing Signalway Antibody, China; Matrigel gel were obtained from Beijing Solarbao Science and Technology Co., Ltd., China; transfection reagents, si-EZH2 and si-NC were synthesized by Ribobio, China; colon carcinoma cell strains HCT-116 were obtained from Shanghai Biomedical, China; HT29 cell strains were obtained from the Shanghai Cell Bank of the Chinese Academy of Sciences.
Si ezh2
Manufactured by RiboBio
Sourced in China
Si-EZH2 is a product designed for the detection and analysis of the EZH2 protein, which is a histone methyltransferase involved in epigenetic regulation. The product provides a solution for researchers to study the expression and function of EZH2 in various biological systems.
Automatically generated - may contain errors
Lab products found in correlation
Si nc, by RiboBio (3 mentions)
Calf serum, by Sartorius (1 mentions)
Cck 8 reagent, by Apexbio (1 mentions)
Mccoy s 5a medium, by Sartorius (1 mentions)
Western blot reagents, by Beyotime (1 mentions)
Si agap2 as1, by RiboBio (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Pcdna3.1 vector, by Thermo Fisher Scientific (1 mentions)
Mir control, by RiboBio (1 mentions)
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Mir 506 3p mimic, by GenePharma (1 mentions)
Liposome 3000 reagent, by Thermo Fisher Scientific (1 mentions)
4 protocols using si ezh2
1
Investigating Colon Cancer Cell Apoptosis
2
Overexpression and silencing of AGAP2-AS1 and TFPI2 in GBM cells
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To overexpress AGAP2-AS1 or TFPI2, the full length cDNA sequences of AGAP2-AS1 or TFPI2 were amplified and subcloned into pcDNA3.1 vector (Invitrogen) according to the manufacture’s guidelines. To suppress gene expression, small interfering RNAs (siRNAs) including si-AGAP2-AS1, si-EZH2, si-LSD1 and si-TFPI2 were purchased from RiboBio (Guangzhou, China). GBM cell were seeded into six-well plates and transfected with plasmids, siRNAs or corresponding negative control (Vector or si-NC) using Lipofectamine 2000 (Invitrogen).
3
Overexpression and Knockdown of LINC00662 and miR-144-3p
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LINC00662 overexpression plasmids (LINC00662; 50 ng), empty vector (vector; 50 ng), miR-144-3p mimics (miR-144-3p, 5′-UUCUCGAACGUGUCACGUUUU-3′; 50 nM), mimics negative control (miR-control, 5′-UCAUGUAGUAGAUAUGACAU-3′; 50 nM), siRNAs targeting EZH2 (si-EZH2, 5′-GAGGGAAAGUGUAUGAUAATT-3′; 100 nM), and siRNAs negative control (si-NC, 5′-GGGAAAGAGUAUAUAGUGATT-3′; 100 nM) were purchased from RiboBio Co., Ltd. (Guangzhou, China). SCC-9 and ISG15 cells were transfected with the oligonucleotide or plasmid employing Lipofectamine 2000 transfection reagent (Invitrogen) according to the protocols provided by the manufacturer.
4
Transfection of miR-506-3p in Colon Cancer Cells
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Cell transfection was performed as previously described [28 (link)]. GenePharma has synthesized miR-506-3p-mimic (5ʹUAAGGCACCCUUCUGAGUAGA-3ʹ), miR-506-3p-inhibitor (5ʹ-UCUACUCAGAAGGGUGCCUUA-3ʹ) and corresponding miR-NC (China), si-NC corresponding to small interfering RNA that targets EZH2 (si-EZH2) purchased from RiboBio Company (China). Based on the kit instructions, these reagents were transfected into HT-29 and HCT-8 cells with Liposome 3000 reagent (Invitrogen), the collected cells for subsequent experiments 72 h after transfection.
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