Maceration was used to extract active components from dried and ground Arthrocnemum macrostachyum samples. Three grams of biomass were added to 100 ml solvent. Two solvents were used for extraction: water and aqueous 50% ethanol (v/v). The biomass and solvent were macerated for 24 h with a speed of 230 rpm using STIK rotary shaker at room temperature. After extraction, the solvent was separated from the solid biomass via vacuum filtration using Supertek 47 mm filtering glass apparatus, Grade 4. Three batches of filtrates were prepared from each crude extract. The filtrates were either dried in the oven at 40 °C (72 h for water – 60 h for aq. EtOH) or 60 °C (48 h for water – 40 h for aq. EtOH) or lyophilized using LABCONCO FreeZone 4.5 Liter (− 100 °C) Benchtop Freeze Dryer for (36 h for water and aq. EtOH). All extraction and drying steps were performed in triplicate.
Freezone 4.5 liter
Manufactured by Labconco
Sourced in United States
The FreeZone 4.5 Liter (-) is a laboratory freeze dryer designed to remove water from samples through the process of lyophilization. It features a 4.5-liter capacity and can operate at temperatures down to -50°C.
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Lab products found in correlation
2 protocols using freezone 4.5 liter
1
Extracting Active Components from Arthrocnemum macrostachyum
2
ANT(6)FMS-007 Enzymatic Characterization
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Each reaction included 5 mM streptomycin in 50 mM NH4HCO3 reaction buffer mixed with 20 μg of ANT(6)FMS-007, 5 mM ATP, and 5 mM Mg2+ in a total volume of 100 μL for 12 h at 37 °C. The products were collected by ultrafiltration using a filter with an MWCO of 3 kDa (Millipore) at 12,000 rpm for 30 min at 4 °C. Ultrafiltrate was vacuum lyophilized (Labconco FreeZone 4.5 Liter, USA) overnight, and samples were resuspended in 15 µL of ultrapure water for MS analysis. The samples were profiled in positive ion reflector mode using an AXIMA MALDI-quadrupole ion trap TOF mass spectrometer (Shimadzu Corp., Japan) with a nitrogen pulsed laser (337 nm) and an acceleration voltage of 20 kV.
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