MDCK-MDR1 cells were seeded on a Merck Transwell polycarbonate membrane (New Jersey, USA) at a density of 2×105 cells/mL. The cells were cultured until tight junctions were formed (trans-epithelial electrical resistance value > 500 Ω·cm2). Before the experiment, the medium on both sides of the chamber was replaced with a warmed HBSS solution. The cells were then equilibrated for 20 min in an incubator. For the transportation test from the apical (AP) to the basolateral (BL) sides, 0.2 mL of HBSS solution containing BBR or the Nnps (both have 10 μg/mL the final concentration of BBR) was added to the AP side, and 0.7 mL blank HBSS solution was added to the BL side. For the transportation test from the BL to AP side, 0.2 mL of blank HBSS solution was added to the AP side and 0.7 mL of HBSS solution containing BBR or the Nnps (both have 10 μg/mL the final concentration of BBR) was added to the BL side. After incubation for 2 or 4 h, the solutions on both sides were aspirated, and the concentration of BBR was determined using the LC-MS/MS method.
Transwell polycarbonate membrane
Manufactured by Merck Group
Sourced in United States
The Transwell polycarbonate membrane is a permeable cell culture insert used for a variety of in vitro assays. It consists of a polycarbonate membrane with precisely controlled pore size and density, allowing for the selective passage of materials between the upper and lower compartments.
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Lab products found in correlation
2 protocols using transwell polycarbonate membrane
1
Transepithelial Transport Study of Berberine
2
BBR Transport Across MDCK-MDR1 Monolayers
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MDCK-MDR1 cells were seeded on a Merck Transwell polycarbonate membrane (NJ, United States) at a density of 2 × 105 cells/mL. The cells were cultured until the tight junctions were formed (transepithelial electrical resistance value >500 Ω cm2). Before the experiment, the media on both sides of the chamber were replaced with a warmed HBSS solution. The cells were then incubated for 20 min at 37°C. For the transportation test from the apical (AP) to the basolateral (BL) sides, 0.2 ml of HBSS solution containing BBR (10 μg/ml) in the absence or presence of 0.3% or 1% of NADES was added to the AP side, and 0.7 ml blank HBSS solution was added to the BL side. For the transportation test from the BL side to the AP side, 0.2 ml blank HBSS solution was added to the AP side and 0.7 ml of HBSS solution containing BBR in the absence or presence of 0.3% or 1% of NADES was added to the BL side. After incubation for 2 or 4 h, the solutions on both sides were aspirated, and the concentration of BBR was determined using the LC-MS/MS method (Quantification of several Coptidis Rhizomaalkaloids).
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