Mouse monoclonal anti-IFI16, rabbit polyclonal anti-p300, mouse monoclonal anti-IL-33 and rat polyclonal anti-BrdU antibodies were from Santa Cruz Biotechnology Inc., Santa Cruz, CA. Rabbit anti-BrdU antibody was from Rockland Inc., Gilbertsville, PA. Mouse monoclonal anti-β-actin and tubulin antibodies plus rabbit anti-human IFI16 antibodies were from Sigma-Aldrich. Mouse monoclonal anti-human IL-1β and caspase-1 antibodies were from R&D Systems, Minneapolis, MN, and Invitrogen, Carlsbad, CA, respectively. Goat polyclonal antibody against human ASC was from RayBiotech. Mouse monoclonal antibody against ASC was from MBL International, Woburn, MA. Mouse anti-human TATA binding protein (TBP), rabbit anti-human Ran and mouse anti-IRF-3 antibodies were from Abcam Inc., Cambridge, MA. Rabbit anti-cyclin B1, rabbit monoclonal anti-STING,-p-IRF-3,-histone H2B and H3 antibodies were from CST, Danvers, MA. Anti-rabbit, goat and mouse antibodies linked to horseradish peroxidase, Alexa Fluor-488, -594 and -647 were from KPL Inc., Gaithersburg, MD, or Molecular Probes, Eugene, OR. Anti-Mouse IgG (heavy-chain spcificity)-HRP conjugate goat antibody was from Alpha Diagnostics Intl. Inc. San Antonio, TX. Anti-mouse tagged with IR Dye 680RD secondary antibodies were from LI COR Biotechnology, Lincoln, NE.
Rabbit polyclonal anti p300
Manufactured by Santa Cruz Biotechnology
Rabbit polyclonal anti-p300 is a laboratory reagent used for the detection and study of the p300 protein in various biological samples. p300 is a transcriptional coactivator involved in the regulation of gene expression. This antibody can be used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to help researchers investigate the role and function of p300 in their areas of study.
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Lab products found in correlation
2 protocols using rabbit polyclonal anti p300
1
Immunoprobing of Cellular Signaling Pathways
2
Immunohistochemical Staining Protocol
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The slides were deparaffinized in xylene, rehydrated through graded alcohol and antigen retrieved by pressure cooking for 30 minutes in ethylenediamine tetraacetic acid (EDTA) buffer for MEF2D; in citrate buffer for p300, phospho-Ser1834-p300 and YY1 and in Trilogy for ZMYM3. The slides were processed following the EnVision + Dual Link Kit (Dako) and incubated overnight at 4°C with rabbit polyclonal anti-p300 (Santa Cruz, 1:50), rabbit polyclonal anti-p-p300 (1:30), rabbit polyclonal anti-YY1 (1:50), rabbit polyclonal anti-ZMYM3 (1:800), mouse monoclonal anti-MEF2D (1:100) diluted in Dako antibody diluent. The slides were stained with DAB (3,3-diaminobenzidine) as described in the protocol and counterstained with Mayer’s hematoxylin, dehydrated, and mounted.
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