HeLa cells (Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University) were cultured in Dulbecco's Modified Eagle's Medium (DMEM, Fujifilm Wako Pure Chemical Corporation), supplemented with 10% FBS (fetal bovine serum, Biosera) and 1% PS solution (penicillin-streptomycin, Fujifilm Wako Pure Chemical Corporation). The day before the cell penetration experiments, cells were harvested from a Petri dish with 0.05% trypsin/EDTA for 2 min at 37 °C and centrifuged at 1400 rpm for 3 min. Then, cells were seeded onto a 35 mm plastic cell culture dish (TPP, Techno Plastic Products AG, Trasadingen, Switzerland), and cultured with DMEM for 24 h. Prior to the measurements, the cell culture medium was replaced with CO2-independent Leibovitz L-15 medium (Fujifilm Wako Pure Chemical Corporation), supplemented with 5% penicillin-streptomycin solution.
Leibovitz l 15 medium
Manufactured by Fujifilm
Sourced in Switzerland
The Leibovitz L-15 medium is a cell culture medium designed for the growth and maintenance of a variety of cell types. It provides the necessary nutrients and growth factors required for cell proliferation and survival. The medium is formulated to maintain a stable pH and osmolarity, supporting optimal cell health and performance.
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2 protocols using leibovitz l 15 medium
1
Culturing HeLa Cells for Penetration Experiments
2
Cell Penetration Experimental Protocol
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HeLa cells (Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University) and BALB/3T3 mouse fibroblasts (JCRB Cell Bank, Japan) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Fujifilm Wako Pure Chemical Corporation), supplemented with 10% fetal bovine serum (FBS, Biosera) and 1% penicillin-streptomycin solution (Fujifilm Wako Pure Chemical Corporation). The day before the cell penetration experiments, cells were harvested from a petri dish with 0.05% trypsin/EDTA for 2 min at 37°C and centrifuged at 1400 rpm for 3 min. Then, cells were seeded onto a 35-mm plastic cell culture dish (TPP Techno Plastic Products AG, Trasadingen, Switzerland) and cultured in DMEM for 24 hours. Before the measurements, the cell culture medium was replaced with CO2-independent Leibovitz L-15 medium (Fujifilm Wako Pure Chemical Corporation), supplemented with 5% penicillin-streptomycin solution. The plastic petri dish with the cultured cells was placed inside the petri dish heater (JPK) preheated to 37°C.
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