5-Bromo-2′-deoxy-uridine (BrdU) labeling and detection kit III was purchased from Roche (Mannheim, Germany). VSMCs were seeded into 96-well culture plates at 1×103 cells/well. At 30% confluence, the cells were starved with serum-free media for 48 h. The cells were stimulated with 15% FBS and pretreated with vehicle or indicated dose of DMF for 24 h. Cells were incubated with BrdU 10 µmol/L for an additional 6 h. The incorporation of BrdU was measured according to the manufacturer׳s instruction.
5 bromo 2 deoxy uridine brdu labeling and detection kit 3
Manufactured by Roche
Sourced in Germany, United States
The 5-Bromo-2′-deoxy-uridine (BrdU) labeling and detection kit III is a laboratory equipment product designed for the detection and quantification of cell proliferation. It allows for the incorporation of BrdU, a synthetic thymidine analog, into newly synthesized DNA during the S-phase of the cell cycle. The kit provides the necessary reagents and protocols to label, detect, and quantify BrdU-positive cells.
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3 protocols using 5 bromo 2 deoxy uridine brdu labeling and detection kit 3
1
BrdU Incorporation Assay for VSMC Proliferation
2
Synthesis and Characterization of Psychoactive Drugs
3
G. procumbens Enhances Cell Proliferation
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Endothelial cells, fibroblasts and keratinocytes treated with 38 mM glucose for 24 h were trypsinized, and 1 × 104 cells/well were cultured in collagen I-coated 96-well plates and stimulated with 50 µg/mL G. procumbens for 48 h at 37 °C. Cell proliferation was assessed using the 5-bromo-2′-deoxyuridine (BrdU) Labeling and Detection Kit III (Roche Diagnostics, Indianapolis, IN, USA) according to the manufacturer’s instructions. In brief, the cells were incubated with 10 µM BrdU for 4 h, and the cells that incorporated BrdU into DNA were detected using monoclonal anti-BrdU-peroxidase Fab fragments. The bound conjugate was visualized with the soluble chromogenic substrate ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)) and measured using a microplate reader at a wavelength of 450 nm [60 (link)].
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