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Lsi egfr spectrum orange cep7 spectrum green probe

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The LSI EGFR Spectrum Orange/CEP7 Spectrum Green probe is a fluorescence in situ hybridization (FISH) probe designed to detect the presence and copy number of the epidermal growth factor receptor (EGFR) gene and the chromosome 7 centromere (CEP7) on human chromosome 7.

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Lab products found in correlation

Axioscop microscope, by Zeiss (2 mentions)

Close spelling variants of this product

Spectrum Green (41 citations) Spectrum Orange (40 citations) EGFR/CEP7 (2 citations)

2 protocols using lsi egfr spectrum orange cep7 spectrum green probe

1

Fluorescence In-Situ Hybridization Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Genes amplifications were investigated by FISH analysis using the LSI EGFR Spectrum Orange/CEP7 Spectrum Green probe (Vysis, Abbott Laboratories, Illinois, USA) and the BAC probe RP11-231c18 directed against PDGFRA (chr4:55,127,335-55,259,498; hg19, spectrum green) and the control probe dj963K6 (4qter, spectrum red).
The FISH signals for each locus-specific FISH probe were assessed under an Zeiss Axioscop microscope (Zeiss,Germany) equipped with specific filters (DAPI/Green/Red). DAPI II (4,6-diamino-2-phenyindole-2-hydrochloride) was used for chromatin counterstaining.
Nikolaev S., Santoni F., Garieri M., Makrythanasis P., Falconnet E., Guipponi M., Vannier A., Radovanovic I., Bena F., Forestier F., Schaller K., Dutoit V., Clement-Schatlo V., Dietrich P.Y, & Antonarakis S.E. (2014). Extrachromosomal driver mutations in glioblastoma and low grade glioma. Nature communications, 5, 5690.
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2

Fluorescence In-Situ Hybridization Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Genes amplifications were investigated by FISH analysis using the LSI EGFR Spectrum Orange/CEP7 Spectrum Green probe (Vysis, Abbott Laboratories, Illinois, USA) and the BAC probe RP11-231c18 directed against PDGFRA (chr4:55,127,335-55,259,498; hg19, spectrum green) and the control probe dj963K6 (4qter, spectrum red).
The FISH signals for each locus-specific FISH probe were assessed under an Zeiss Axioscop microscope (Zeiss,Germany) equipped with specific filters (DAPI/Green/Red). DAPI II (4,6-diamino-2-phenyindole-2-hydrochloride) was used for chromatin counterstaining.
Nikolaev S., Santoni F., Garieri M., Makrythanasis P., Falconnet E., Guipponi M., Vannier A., Radovanovic I., Bena F., Forestier F., Schaller K., Dutoit V., Clement-Schatlo V., Dietrich P.Y, & Antonarakis S.E. (2014). Extrachromosomal driver mutations in glioblastoma and low grade glioma. Nature communications, 5, 5690.
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