Fisherbrand traceable digital caliper

Manufactured by Thermo Fisher Scientific

Sourced in United States

Fisherbrand Traceable digital calipers are precision measuring tools used to accurately measure dimensions, such as length, depth, and thickness. They feature a large, easy-to-read digital display and provide measurements in both standard and metric units.

Automatically generated - may contain errors

Lab products found in correlation

Ethyl carbamate, by Merck Group (3 mentions) Bouin s fixative solution, by Merck Group (2 mentions) Pa84 precision balance, by Ohaus (2 mentions) Uric acid, by Merck Group (1 mentions) Lal endotoxin assay, by GenScript (1 mentions)

Spelling variants of this product

Digital calipers (54 citations) Traceable Digital Calipers (12 citations) Fisherbrand Traceable (2 citations)

10 protocols using fisherbrand traceable digital caliper

Urethane-Induced Lung Tumor Model

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tumors were induced in 8-month-old mice by a single intraperitoneal injection of 1 mg/g urethane (ethyl carbamate; Sigma-Aldrich, St. Louis, MO, USA) once a week for 10 weeks. Ten mice per group were euthanized at time points of up to 6 months after the injection of the carcinogen. After fixation, the lungs were used for surface tumor number and diameter measurements. Tumors on the lung surface were enumerated by at least two experienced readers, who were blinded to sample identifiers under a dissecting microscope; tumor counts were averaged and statistically analyzed. Tumor diameters were measured using Fisherbrand Traceable Digital Calipers (Fisher Scientific, Asheville, NC, USA).

Lee D.H., Kim K.C., Hwang C.J., Park K.R., Jung Y.S., Kim S.Y., Kim J.Y., Song J.K., Song M.J., Choi M.K., Hwang D.Y., Han S.B, & Hong J.T. (2019). Decreased Lung Tumor Development in SwAPP Mice through the Downregulation of CHI3L1 and STAT 3 Activity via the Upregulation of miRNA342-3p. Molecular Therapy. Nucleic Acids, 16, 63-72.

+ Open protocol

+ Expand

Skin Carcinogenesis Model in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Eight-week old WT and IL-32γ mice were used. Skin carcinogenesis was performed as previously described [52 (link)]. The dorsal skin of the mice was shaved, and the exposed areas were treated with 25 μg DMBA (Sigma, St. Louis, MO, USA) in 200 μL of acetone per mouse. After 1 week, 5 μg TPA (Sigma, St. Louis, MO, USA) in 200 μL of acetone was applied per mouse thrice a week. Mice were evaluated weekly for papilloma development. Mice were euthanized after a 25-week TPA treatment. At the time of sacrifice, the skin was fixed in 4% formalin solution. After fixation, the skin was used for surface tumor number and diameter measurements and then embedded in paraffin. Tumor counts were averaged and statistically analyzed. Tumor diameters were measured using Fisherbrand Traceable digital calipers (Fisher Scientific, Asheville, NC, USA). Then, skin tissues were embedded in paraffin. To induce skin inflammation, single TPA was applied on the shaved mice. The shaved mice were treated with or without 0.1 μmol Bay 11–7082 in 200 μL of acetone per mouse. After 1 h, mice were treated with 10 μg TPA in 200 μL of acetone per mouse. Mice were sacrificed after 24 h.

Lee Y.S., Lee C.H., Bae J.T., Nam K.T., Moon D.B., Hwang O.K., Choi J.S., Kim T.H., Jun H.O., Jung Y.S., Hwang D.Y., Han S.B., Yoon D.Y, & Hong J.T. (2018). Inhibition of skin carcinogenesis by suppression of NF-κB dependent ITGAV and TIMP-1 expression in IL-32γ overexpressed condition. Journal of Experimental & Clinical Cancer Research : CR, 37, 293.

+ Open protocol

+ Expand

Urethane-Induced Lung Tumor Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Eight-week-old IL-32γ mice were used. Tumors were induced by a single i.p. injection of 1 mg/g urethane (ethyl carbamate; Sigma-Aldrich, St. Louis, MO) once a week for 10 weeks. Mice were euthanized at time points up to 6 months after injection of carcinogen. At the time of sacrifice, lungs were lavaged, perfused, and fixed in ice-cold Bouin’s fixative solution (Sigma-Aldrich) for 24 h. After fixation, lungs were used for surface tumor number and diameter measurements, and embedded in paraffin. Tumors on the lung surface were enumerated by at least two experienced readers, blinded to sample identifiers under a dissecting microscope; tumor counts were averaged and statistically analyzed. Tumor diameters were measured using Fisherbrand Traceable digital calipers (Fisher Scientific, Asheville, NC).

Yun J., Park M.H., Son D.J., Nam K.T., Moon D.B., Ju J.H., Hwang O.K., Choi J.S., Kim T.H., Jung Y.S., Hwang D.Y., Han S.B., Yoon D.Y, & Hong J.T. (2018). IL-32 gamma reduces lung tumor development through upregulation of TIMP-3 overexpression and hypomethylation. Cell Death & Disease, 9(3), 306.

+ Open protocol

+ Expand

Urethane-Induced Lung Tumor Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Eighteen to twenty-week-old mice were used. Tumors were induced by a single intraperitoneal injection of 1 mg/g urethane (ethyl carbamate; Sigma-Aldrich, St Louis, MO, USA) once a week for 10 weeks. Mice were euthanized at time points up to 6 months after injection of carcinogen. At the time of sacrifice, lungs were lavaged, perfused and fixed in ice-cold Bouin's fixative solution (Sigma-Aldrich) for 24 h. After fixation, lungs were used for surface tumor number and diameter measurements, and embedded in paraffin. Tumors on the lung surface were enumerated by at least two experienced readers, blinded to sample identifiers under a dissecting microscope; tumor counts were averaged and statistically analyzed. Tumor diameters were measured using Fisherbrand Traceable digital calipers (Fisher Scientific, Ashville, NC, USA).

Yun H.M., Park M.H., Kim D.H., Ahn Y.J., Park K.R., Kim T.M., Yun N.Y., Jung Y.S., Hwang D.Y., Yoon D.Y., Han S.B, & Hong J.T. (2014). Loss of presenilin 2 is associated with increased iPLA2 activity and lung tumor development. Oncogene, 33(44), 5193-5200.

+ Open protocol

+ Expand

Silicone Tube Characterization after Infusion

Check if the same lab product or an alternative is used in the 5 most similar protocols

The length, inner diameter, and outer diameter of silicone tubes were measured before silicone oil infusion and following complete infusion (after incubating with silicone oil for >5 days). All parameters were measured using a digital caliper (06-664-16, Fisherbrand Traceable Digital Calipers, Fisher Scientific, USA). For mouse catheters, length was measured using a digital caliper. For inner and outer diameter measurement, photos of the tube openings of the catheters and a scale of known length were taken and estimated using ImageJ. Percentage weight change of Tygon tube, silicone tube, and mouse catheters were calculated based on the formula below:

\frac{W e i g h t o f t h e t u b e - I n i t i a l w e i g h t o f t h e t u b e}{I n i t i a l w e i g h t o f t h e t u b e} * 100 %

Andersen M.J., Fong C., La Bella A.A., Molina J.J., Molesan A., Champion M.M., Howell C, & Flores-Mireles A.L. (2022). Inhibiting host-protein deposition on urinary catheters reduces associated urinary tract infections. eLife, 11, e75798.

+ Open protocol

+ Expand

Feline Linea Alba Width Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cadavers were placed into a freezer within 30 min of euthanasia and stored at −80° C until testing. Twenty four hours prior to testing, cadavers were removed from the freezer and allowed to thaw at room temperature. Once thawed, the body weight (BW), sex, and breed were recorded. All cats were positioned in dorsal recumbency, and after clipping the hair, the ventral body wall was visually inspected to identify any criteria warranting exclusion from the study.
The entire length of the linea alba and ventral body wall cranial and caudal to it were exposed by incising the skin of the ventral abdomen, and reflecting the subcutaneous soft tissues (Figure 1A). Once exposed, the width of the linea alba was measured with digital calipers (Fisherbrand™ Traceable™ Digital Calipers, Thermo Fisher Scientific) at the cranial abdominal (CrA), umbilical (U), and caudal abdominal (CdA) regions. At each location, the width of the linea alba was measured three times and the average of these measurements was recorded.

Reina Rodriguez F.S., Milgram J, & Kirby B.M. (2020). Small vs. Large Suture Bite-to-Stitch Interval for Closure of Midline Celiotomy in Cats: A Biomechanical Study. Frontiers in Veterinary Science, 7, 206.

+ Open protocol

+ Expand

Preparation and Use of MSU Crystals

Check if the same lab product or an alternative is used in the 5 most similar protocols

MSU crystals were prepared as described previously (2 (link)). 1 g of uric acid (Sigma) in 180 ml 0.01 M NaOH was heated to 70° C. NaOH was added as required to maintain pH between 7.1 and 7.2 and the solution was filtered and incubated at RT with slow and continuous stirring for 24 h. MSU crystals were kept sterile, washed with ethanol, dried, autoclaved and re-suspended in PBS by sonication. MSU crystals contained < 0.005 EU/mL endotoxin (LAL endotoxin assay, GenScript).
In most experiments (and unless stated otherwise), we injected 0.5 mg MSU i.a. in 10 μl PBS in one ankle and PBS only in the contra-lateral ankle. We used Microliter Syringes #705 (Hamilton) with 27G needles for all i.a. injections. Injections were performed under isoflurane anesthesia, and the quality of i.a. injection was controlled by assessing location of MSU crystals deposition by histology on ankle tissue collected 24 h after the injection. In some experiments, we used MC-deficient mice engrafted with WT BMCMCs in one ankle and IL-1β^−/− BMCMCs in the contra-lateral ankle and injected these mice with MSU crystals in both ankles (Figure 3, A and B). We also injected DT-treated Cpa3-Cre; iDTR mice with MSU crystals in both ankles (Figure 4, F and G). Ankle swelling was measured at different time points using a precision caliper (Fisherbrand Traceable Digital Calipers; Fischer Scientific).

Reber L.L., Marichal T., Sokolove J., Starkl P., Gaudenzio N., Iwakura Y., Karasuyama H., Schwartz L.B., Robinson W.H., Tsai M, & Galli S.J. (2014). Mast cell-derived IL-1β contributes to uric acid crystal-induced acute arthritis in mice. Arthritis & rheumatology (Hoboken, N.J.), 66(10), 2881-2891.

+ Open protocol

+ Expand

Preclinical Modeling of Tumor-Immune Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols

B16-OVA melanoma cells (1 × 10⁶ per animal) or MC38 colon cancer cells (5 × 10⁵ per animal) were subcutaneously (s.c.) injected into experimental mice to form solid tumors. In some experiments, mice received an intraperitoneal (i.p.) injection of phenelzine (30 mg/kg/day) to block MAO-A activity. In some experiments, mice received i.p. injection of clodronate liposomes (200 μl/animal, twice per week) to deplete TAMs; mice received i.p. injection of vehicle liposomes (200 μl/animal, twice per week) were included as controls. In some experiments, mice received i.p. injection of anti-mouse PD-1 antibodies (300 μg/animal, twice per week) to block PD-1; mice received i.p. injection of isotype antibodies was included as controls. During an experiment, tumor growth was monitored twice per week by measuring tumor size using a Fisherbrand^TM Traceable^TM digital caliper (Thermo Fisher Scientific); tumor volumes were calculated by formula 1/2 × L × W². At the end of an experiment, solid tumors were collected and tumor-infiltrating immune cells were isolated for analysis using QPCR, flow cytometry, and/or scRNASeq.

Wang Y.C., Wang X., Yu J., Ma F., Li Z., Zhou Y., Zeng S., Ma X., Li Y.R., Neal A., Huang J., To A., Clarke N., Memarzadeh S., Pellegrini M, & Yang L. (2021). Targeting monoamine oxidase A-regulated tumor-associated macrophage polarization for cancer immunotherapy. Nature Communications, 12, 3530.

+ Open protocol

+ Expand

Melanoma Regression in NSG Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

NSG mice were subcutaneously inoculated with 1 × 10⁶ A375-CD1d-FG cells (day 0) and were allowed to develop melanoma over the course of about 3 weeks. Three days post-tumor inoculation (day 3), mice received the i.v. injection of vehicle (PBS) or 1 × 10⁷ PBMC-iNKT cells with or without the administration of tumor-localized αGC (10 μg/mL; 100 μL/mouse). Over time, tumor loads in experimental animals were monitored starting from day 2 by measuring total body luminescence using BLI (shown as TBL p/s) and by measuring tumor volume using a Fisherbrand^TM Traceable^TM digital caliper (Thermo Fisher Scientific). Tumor volume was calculated as width² × length × 0.52 (mm³). At approximately week 3, mice were terminated. Solid tumors were retrieved, weighted using a PA84 precision balance (Ohaus), and then compared.

Li Y.R., Zhou Y., Wilson M., Kramer A., Hon R., Zhu Y., Fang Y, & Yang L. (2022). Tumor-Localized Administration of α-GalCer to Recruit Invariant Natural Killer T Cells and Enhance Their Antitumor Activity against Solid Tumors. International Journal of Molecular Sciences, 23(14), 7547.

+ Open protocol

+ Expand

Therapeutic Efficacy of PBMC-iNKT Cells in Lung Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols

NSG mice were subcutaneously inoculated with 1 × 10⁶ H292-CD1d-FG cells (day 0) and were allowed to develop lung cancer over the course of about 5 weeks. Three days post-tumor inoculation (day 3), mice received the i.v. injection of vehicle (PBS) or 1 × 10⁷ PBMC-iNKT cells with or without the administration of tumor-localized αGC (10 μg/mL; 100 μL/mouse). Over time, tumor loads in experimental animals were monitored starting from day 2 by measuring total body luminescence using BLI (shown as TBL p/s) and by measuring tumor volume using a Fisherbrand^TM Traceable^TM digital caliper (Thermo Fisher Scientific). Tumor volume was calculated as width² × length × 0.52 (mm³). At around week 5, mice were terminated. Solid tumors were retrieved, weighted using a PA84 precision balance (Ohaus), and then compared.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!