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Rabbit polyclonal antibodies specific for β actin

Manufactured by Santa Cruz Biotechnology
Sourced in United States

Rabbit polyclonal antibodies specific for β-actin. These antibodies are designed to recognize and bind to the β-actin protein, a widely expressed cytoskeletal protein found in eukaryotic cells.

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2 protocols using rabbit polyclonal antibodies specific for β actin

1

Investigating Cellular Signaling Pathways

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Rabbit polyclonal antibodies specific for β-actin, IL-18, p-FAK, FAK, p-p85, p85, p-Akt, and Akt were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Fetal bovine serum (FBS), DMEM, and other cell culture reagents were obtained from Gibco-BRL Life Technologies (Grand Island, NY, USA). Recombinant human YKL-40 was obtained from PeproTech (Rocky Hill, NJ, USA). miR-590-3p mimic and control miRNA were obtained from GeneDireX (Las Vegas, NV, USA). All pharmacologic inhibitors were obtained from Sigma-Aldrich (St. Louis, MO, USA).
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2

Signaling Pathway Analysis in Cells

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Rabbit polyclonal antibodies specific for β-actin, CCL2, p-ERK, ERK, p-p38, p38, p-JNK, JNK, and CD68 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Mouse monoclonal antibody specific for CCN1 was purchased from R&D Systems (Minneapolis, MN). Recombinant human CCN1 was purchased from PeproTech (Rocky Hill, NJ). Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), and all other cell culture reagents were purchased from Gibco-BRL Life Technologies (Grand Island, NY). The dual-luciferase reporter assay kit was purchased from Promega (Madison, WI, USA). Negative control miRNA and human miR-518a-5p mimic were purchased from GeneDireX (Las Vegas, NV). All chemicals were purchased from Sigma-Aldrich (St. Louis, MO).
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