Cell viability and caspase activity was determined using the CellTitre-Glo luminescent viability kit and caspase Glo assays (Promega, Madison, USA) as described in the manufacturer’s instructions.
Celltitre glo luminescent viability kit
Manufactured by Promega
Sourced in United States
The CellTiter-Glo luminescent viability kit is a cell-based assay that quantifies ATP, which indicates the presence of metabolically active cells. The kit uses a luminescent substrate that produces light in the presence of ATP, enabling the measurement of cell viability and proliferation.
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Lab products found in correlation
2 protocols using celltitre glo luminescent viability kit
1
Cell Viability and Caspase Assay
2
Determining Synergistic Drug Combinations
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Cell viability and caspase activity was determined using the CellTitre-Glo® luminescent viability kit and caspase Glo® assays (Promega, Madison, USA) as described in the manufacturer's instructions. Calcusyn Software Version 2.1 (Cambridge Computing Imaging Ltd, Cambridge, UK) was used to calculate the combination index (CI) using the percentage of cells affected. The Chou-Talalay method [62 (link)] is employed by Calcusyn for calculating the CI. The software uses a method based on the median effect equation (MEE) derived from the mass action law and correlates dose and effect to produce the CI value; anything less than one showing a level of synergy (closer the number to 0 shows a greater degree of synergy) or a number greater than 1 showing antagonism between the agents. In this case we used the percentage of non-viable cells after treatment (as determined by the CellTitre-Glo® assay described above) as the fractional affect. This data is input into the software along with the dose of all agents used in the combination treatment.
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