Ml 845 powerlab

Transthoracic echocardiography was performed using the Vivid I ultrasound cardiovascular system (GE Healthcare, Haifa, Israel) under isoflurane anesthesia (5% for induction and 2% for maintenance) at 10 and 13 weeks of age in the control and also in the DM rats with and without MPT0E014 administration. M-Mode tracing of the left ventricle (LV) was used to measure the following cardiac structures: the LV end-diastolic diameter (LVEDd), LV end-systolic diameter (LVESd), interventricular septal thickness in diastole (IVSd), end-diastolic volume (EDV), end-systolic volume (ESV), fractional shortening (FS), and ejection fraction (EF) [10 (link)].
Electrocardiograms (ECGs) were performed at 10 and 13 weeks of age and were recorded from standard lead II limb leads via a bioamplifier (ADInstruments, Castle Hill, Australia), connected to a polygraph recorder (ML 845 Powerlab, ADInstruments). Results were continuously displayed throughout the experiment in the control as well as the DM rats with and without MPT0E014 treatment.

Under isoflurane anesthesia (5% for induction and 2% for maintenance), transthoracic echocardiography was performed using a Vivid I ultrasound cardiovascular system (GE Healthcare, Haifa, Israel) at 16 weeks of age (6 weeks after receiving STZ) in the control and DM rats, with and without empagliflozin treatment. The following cardiac structures were measured using M-Mode tracing of the LV: LVEDd, LVESd, EDV, ESV, FS, and EF [46 (link)].
Electrocardiography was performed at 10 and 16 weeks of age, and electrocardiograms were recorded from standard lead II limb leads via a bio-amplifier (AD Instruments, Castle Hill, Australia), connected to a polygraph recorder (ML 845 Powerlab, AD Instruments) [47 (link)]. The results were continuously displayed throughout the experiments in the control and DM rats, with and without empagliflozin treatment.
The systolic and diastolic BPs of the control and DM rats with and without empagliflozin treatment, were measured at 10 and 16 weeks of age using a non-invasive BP tail-cuff method (MK-2000, Muromachi Kikai, Tokyo, Japan) as described previously [47 (link)].