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Pbs buffer

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1X PBS buffer is a commonly used aqueous solution that provides a physiologically compatible environment for various biological and biochemical applications. It is a balanced salt solution that maintains a neutral pH and helps maintain the stability and integrity of biological samples or reactions.

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Lab products found in correlation

Zetaview pmx 110, by Particle Metrix (17 mentions) Ultrasonic homogenizer, by Sonics (1 mentions) Zetaview, by Particle Metrix (1 mentions) Zetaview analyzer, by Particle Metrix (1 mentions) Zetasizer nano zs90, by Malvern Panalytical (1 mentions) Nanosight ns300 system, by Malvern Panalytical (1 mentions)

Spelling variants of this product

Phosphate buffer saline (3 citations) Phosphate buffer solution (2 citations)

24 protocols using pbs buffer

1

Characterizing OMVs of P. gingivalis

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The diameters and the numbers of OMVs produced by P. gingivalis (W83) in the pre-log, late-log, and stationary stages were measured by nanoparticle tracking analysis (NTA). The specific steps are as follows: Firstly, we took freshly extracted OMVs 50μl preparation for testing and cleaned the sample cell with deionized water; Then, the instrument was calibrated using polystyrene microspheres (110nm) and cleaned the sample pool with 1 × PBS buffer (Biological Industries, Israel); Finally, we used 1 × PBS buffer to dilute the sample for testing. Repeat testing three times for each sample.
The morphology of P. gingivalis OMVs were observed via transmission electron microscopy (TEM). Freshly extracted OMVs was diluted 500 times and transported on ice; Secondly, we dropped OMVs diluted to a suitable magnification onto the electron microscope copper mesh grid and waited for 10 minutes; Thirdly, we added 2% uranyl acetate dropwise to the copper net for 3 minutes, and cleaned twice with deionized water; Finally, take photos under a transmission microscope.
Mao H., Gong T., Sun Y., Yang S., Qiao X, & Yang D. (2023). Bacterial growth stage determines the yields, protein composition, and periodontal pathogenicity of Porphyromonas gingivalis outer membrane vesicles. Frontiers in Cellular and Infection Microbiology, 13, 1193198.
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2

Measuring uEV Size and Concentration

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The particle size and concentration of uEVs were measured using nanoparticle tracking analysis (NTA) at VivaCell Biosciences with ZetaView PMX 110 (Particle Metrix, Meerbusch, Germany) and the corresponding software ZetaView 8.04.02. Briefly, uEVs were appropriately diluted in 1× PBS buffer (Biological Industries, Israel) to measure particle size and concentration. After the sample cell was cleaned using 1× PBS buffer (Biological Industries, Israel), the ZetaView system was calibrated using 110 nm polystyrene particles.
Kong L., Tang X., Kang Y., Dong L., Tong J., Xu J., Gao P.J., Wang J.G., Shen W, & Zhu L. (2022). The Role of Urinary Extracellular Vesicles Sodium Chloride Cotransporter in Subtyping Primary Aldosteronism. Frontiers in Endocrinology, 13, 834409.
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3

Degradation of Aflatoxin B1 by M. proteolyticum

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The degradation of AFB1 by different components of M. proteolyticum B204 was investigated as described below, referring to Wang et al. with minor modifications [2 (link)]. Cell-free culture supernatant was collected after centrifugation at 8000 rpm for 20 min at 4 °C, followed by filtration through 0.22 μm filters and preserved on ice for a further AFB1 degradation assay. The precipitation of bacteria was thoroughly washed by sterilized MilliQ water and re-dissolved in PBS buffer (Biological Industries, Kibbutz Beit Haemek, Israel). The resuspending cells were then disrupted by ultrasonic homogenizer (Sonics, Wallingford, CT, USA) at 28% power with a 4 s pulse on and 6 s pulse off repetition cycle on ice. Cell-free extracts were obtained after centrifugation at 8000 rpm for 20 min at 4 °C and filtration through 0.22 μm filters. AFB1 standard solution was then introduced into cell-free culture supernatant, cell-free extracts and cells with a final concentration of 10 μg/μL, respectively. LB culture medium + PBS buffer with equivalent AFB1 was set as a negative control. After incubation at 30 °C for 24 h, the residual AFB1 was detected by HPLC as described in Section 5.3.
Yan Y., Zhang X., Chen H., Huang W., Jiang H., Wang C., Xiao Z., Zhang Y, & Xu J. (2022). Isolation and Aflatoxin B1-Degradation Characteristics of a Microbacterium proteolyticum B204 Strain from Bovine Faeces. Toxins, 14(8), 525.
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4

Nanoparticle Tracking Analysis of SPEV Size and Concentration

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We measured the SPEVs particle size and concentration using nanoparticle tracking analysis (NTA) at VivaCell Biosceinces with ZetaView PMX 110 (Particle Metrix, Meerbusch, Germany) and the corresponding software ZetaView 8.04.02. Isolated exosome samples were appropriately diluted using 1 × PBS buffer (Biological Industries, Kibbutz Beit Haemek, Israel) before the measurement. NTA measurement was recorded and analyzed at 11 positions. The ZetaView system was calibrated using 110 nm polystyrene particles. Temperature was maintained around 26°C.
Han X., Li Y., Zong Y., Li D., Yuan J., Yang H., Ma H., Ni A., Wang Y., Zhao J., Chen J., Ma T, & Sun Y. (2023). Extracellular vesicle-coupled miRNA profiles of chicken seminal plasma and their potential interaction with recipient cells. Poultry Science, 102(12), 103099.
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5

Exosome Characterization via NTA

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We used ZetaView PMX 110 (Particle Metrix, Meerbusch, Germany) and the respective software ZetaView 8.04.02 to measure exosome particle size and concentration using nanoparticle tracking analysis (NTA). Isolated exosome samples were diluted to assess particle size and concentration using PBS buffer (Biological Industries, Kibbutz Beit Haemek, Israel). The measurement of NTA at 11 positions was registered and analyzed. The ZetaView device used 110 nm of polystyrene particles for calibration. Approximately 37°C preserved temperature.
Xu L., Liao W.L., Lu Q.J., Zhang P., Zhu J, & Jiang G.N. (2021). Hypoxic tumor-derived exosomal circular RNA SETDB1 promotes invasive growth and EMT via the miR-7/Sp1 axis in lung adenocarcinoma. Molecular Therapy. Nucleic Acids, 23, 1078-1092.
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6

Nanoparticle Tracking Analysis of EVs

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The size and concentration of EVs were measured using nanoparticle tracking analysis (NTA) at VivaCell Biosceinces with ZetaView PMX 110 (Particle Metrix, Meerbusch, Germany) and corresponding software ZetaView 8.04.02. Isolated EVs samples were appropriately diluted using PBS buffer (Biological Industries, Kibbutz, Israel) to measure the particle size and concentration. NTA measurement was recorded and analyzed at 11 positions. The ZetaView system was calibrated using 110 nm polystyrene particles. Temperature was maintained around 23°C and 30°C.
Wu Y., Yu Q., Zhang M., Zhou Y., Su X., Wu M., Lv J, & Xia Z. (2021). Hemin‐primed dendritic cells suppress allergic airway inflammation through releasing extracellular vesicles. Journal of Leukocyte Biology, 111(4), 837-848.
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7

Exosome Sample Preparation Protocol

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The exosomes sample pool was washed with deionized water. ZetaView Particle Metrix (Particle Metrix, Germany) is calibrated with polystyrene microspheres with a size of 110 nm. Then, the sample pool was washed with 1 × PBS. Finally, the exosomes sample was diluted by 1 × PBS buffer (Biological Industries, Israel) to for testing.
Yang S., Guo S., Tong S, & Sun X. (2020). Exosomal miR‐130a‐3p regulates osteogenic differentiation of Human Adipose‐Derived stem cells through mediating SIRT7/Wnt/β‐catenin axis. Cell Proliferation, 53(10), e12890.
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8

Characterization of Platelet-Derived Small Extracellular Vesicles

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The morphology of the obtained P-sEVs was determined by TEM (transmission electron microscope), and 15 ​μL of the P-sEV suspension was dropped onto copper grids covered with a carbon support film (Zhongjingkeyi Technology, China). The samples were air-dried for 1 ​min at room temperature, and then, excess fluid was removed with filter paper. The samples were negatively stained with 2% uranyl acetate for 1 ​min. After that, the stained samples were baked under a lamp for 10 ​min. Finally, TEM (FEI, USA) was performed at 200 ​kV to visualize and examine the morphology of the P-sEVs.
The particle size and concentration of P-sEVs were measured using nanoparticle tracking analysis (NTA) at VivaCell Shanghai with ZetaView PMX 110 (Particle Metrix, Meerbusch, Germany) and corresponding software ZetaView 8.04.02. Isolated P-sEV samples were appropriately diluted using 1X PBS buffer (Biological Industries, Israel) to measure the particle size and concentration. NTA measurements were recorded and analyzed at 11 positions. The ZetaView system was calibrated using 110 ​nm polystyrene particles. The temperature was maintained at approximately 23 ​°C.
Han Z., Dong L., Li A., Li Z., Fu L., Zhang Z., Li X, & Li X. (2022). Efficient angiogenesis-based wound healing through hydrogel dressing with extracellular vesicles release. Materials Today Bio, 16, 100427.
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9

Nanoparticle Tracking Analysis of Exosomes

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Exosome size and concentration were measured by using NTA with a ZetaView PMX 110 analyser (Particle Metrix, Meerbusch, Germany) and ZetaView 8.04.02 software. Briefly, isolated exosome samples were appropriately diluted using 1x PBS buffer (Biological Industries, Israel) to measure the particle size and concentration. NTA was performed at 11 positions. The ZetaView system was calibrated using 110 nm polystyrene particles. The temperature was maintained at approximately 25 °C to 26 °C.
Jiang T., Xu C., Gao S., Zhang J., Zheng J., Wu X., Lu Q., Cao L., Yang D., Xu J, & Chen X. (2022). Cathepsin L-containing exosomes from α-synuclein-activated microglia induce neurotoxicity through the P2X7 receptor. NPJ Parkinson's Disease, 8, 127.
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10

Exosome Characterization Using NTA

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NTA was applied to identify whether pellets were exosomes by detecting the particle size. Firstly, pellets were washed using deionized water. Then, polystyrene microspheres were applied to calibrate ZetaView analyzer (Particle Metrix, Meerbusch, Germany). Next, pellets were diluted with 1X PBS buffer (Biological Industries, Israel). After that, ZetaView analyzer was conducted to detect whether these pellets conformed to the structure and particle size of the exosomes according to the previous literature [40 (link),41 (link)].
Chen Y., Hong C., Qu J., Chen J, & Qin Z. (2022). Knockdown of lncRNA PCAT6 suppresses the growth of non-small cell lung cancer cells by inhibiting macrophages M2 polarization via miR-326/KLF1 axis. Bioengineered, 13(5), 12834-12846.
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