M. oleifera gum (MOG) was collected from Srinagar, Uttarakhand, India. Doxorubicin hydrochloride (DOX, Samarth Life Sciences Pvt. Ltd., Mumbai, Maharashtra, India), disodium hydrogen orthophosphate anhydrous (Na2HPO4), sodium dihydrogen orthophosphate dihydrate (NaH2PO4.2H2O), N,N’-bismethyleneacrylamide (N,N’-MBA), acrylamide (AAm), methanol, hydrochloric acid (HCl), sodium hydroxide (NaOH), dimethyl sulfoxide (DMSO) (S.D Fine–Chem Ltd., Mumbai, Maharashtra, India), Dulbecco’s Modified Eagle’s Medium (DMEM), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), pyridoxal 5’-phosphate (PLP), fetal bovine serum (FBS), penicillin and streptomycin (HiMedia Lab. Pvt. Ltd., Mumbai, Maharashtra, India) and Rhabdomyosarcoma cells (RD cells, National Culture for Cell Science (NCCS), Pune, Maharashtra, India), were of analytical grade. These were used as received. Double distilled water was used in all the experiments.
Penicillin and streptomycin
Manufactured by HiMedia
Sourced in India
Penicillin and streptomycin are antibiotics that are commonly used in laboratory settings. Penicillin is a beta-lactam antibiotic that inhibits the synthesis of bacterial cell walls, while streptomycin is an aminoglycoside antibiotic that interferes with bacterial protein synthesis. These antibiotics are often used in cell culture media to prevent bacterial contamination.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by HiMedia (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Tgf β, by R&D Systems (1 mentions)
Hoechst, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Trypsin, by Merck Group (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Colchicine, by Merck Group (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
17β estradiol e2, by Merck Group (1 mentions)
Dual luciferase assay kit, by Promega (1 mentions)
Clarity western ecl substrate, by Bio-Rad (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by HiMedia (1 mentions)
Fbs fetal bovine serum, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Zeocin, by InvivoGen (1 mentions)
Blasticidin, by InvivoGen (1 mentions)
7 protocols using penicillin and streptomycin
1
Synthesis and Characterization of M. oleifera Gum-based Hydrogel
2
Estrogen Receptor Knockdown in Breast Cancer Cells
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ER‐positive cell lines, MCF‐7 and T47D cells were used in this study. The cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) with 10% fetal bovine serum (FBS) (Invitrogen) and 1% penicillin and streptomycin (HiMedia) at 37°C with 5% CO2. The cells were transfected at around 70%–80% confluency with lipofectamine 2000 (Invitrogen) according to manufacturer's instruction using ERαsiRNA sc‐29305 with OptiMeM medium followed by medium change after 6 h of transfection. The cells were either treated with 27‐HC (1 μM) or DMSO (0.1%) for various time points. The cells were maintained in phenol‐red free DMEM (Sigma Cat#D1152) supplemented with 5% charcoal‐stripped serum 72 h prior to treatment. The treatments were done in phenol‐red free DMEM supplemented with 5% charcoal‐stripped serum.
3
Liver Transcriptome Analysis of Cirrhosis Patients
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All the human liver samples were obtained from the liver clinic patients came to Institute of Liver and Biliary Sciences, New Delhi. The samples were obtained after the Institutional human ethical committee approval and after obtaining written consent from the participants. The control samples (n = 5) were obtained from the healthy liver donors who came for liver transplantation and the cirrhotic liver samples were obtained from the liver transplant recipient patients (n = 5). These samples were homogenized and used for total RNA isolation and real-time PCR. Human stellate cell line, LX2, were cultured in Dulbecco’s Minimal Essential Media (DMEM) (HiMedia Laboratories, Mumbai, India) supplemented with 10% fetal bovine serum (Invitrogen, Carlsbad, CA, USA) and 1 mg/ml penicillin and streptomycin (HiMedia Laboratories) at 37°C and 5% CO2. After 24 h, the media was washed and TGF-β (20 ng/ml) (R & D Systems, Minneapolis, MN, USA) was added to the cells in serum free DMEM. After 24 h of the incubation, the cells were washed, collected either for protein isolation or for RNA purification.
4
Cell Culture Maintenance for MCF-7 and MDA-MB 231
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MCF-7 and MDA-MB 231 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin and streptomycin (HiMedia, Cat # A002A) at 37°C with 5% CO2. The cells were always treated with phenol red less medium containing 5% charcoal-treated serum before proceeding with 27-HC and Dimethylsulfoxide (DMSO) treatment.
5
Cell Viability and Apoptosis Assay
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Plastic wares (Tarson), MTT (Sigma), sterile dimethyl sulfoxide (DMSO) (Merck), RPMI 1640 medium (Sigma), fetal bovine serum (FBS) (Sigma), trypsin (Sigma), penicillin and streptomycin (Himedia), acridine orange and ethidium bromide (Sigma), Hoechst (Sigma), JC-1 (Sigma) and caspase-3 assay kit (Raybiotech) were purchased for this study.
6
Estrogen Receptor Signaling in MCF-7 Cells
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MCF-7 cells were a kind gift from Dr. Dipak Datta, Central Drug Research Institute, Lucknow, India. Cell culture plasticware were purchased from Eppendorf AG (Hamburg, Germany). Fetal bovine serum (FBS) were from Invitrogen Corporation (USA). Cell culture media and charcoal-stripped FBS (csFBS), trypsin, penicillin and streptomycin, nitrocellulose membrane, and MTT were purchased from HiMedia (Mumbai, India). Lipofectamine 3000 was from Invitrogen Corporation (USA). Progesterone receptor (PR) antibody (Catalog No.-3176S) and anti-rabbit secondary antibody (Catalog No.-7074S) were purchased from Cell Signaling (Beverly, MA). β-Actin antibody (Catalog No.- AM4302) was purchased from Invitrogen Corporation (USA). Histone antibody (Catalog No.- BB-AB0055) was from BioBharati Life sciences (India). 3xERRE/ERE-luciferase was a kind gift from Rebecca Riggins (Addgene plasmid # 37852). pRL-SV40P was a gift from Ron Prywes (Addgene plasmid # 27163). 17β-estradiol (E2) and colchicine were from Sigma Aldrich (USA). Clarity Western ECL Substrate was purchased from Bio-Rad (USA). Dual luciferase assay kit was procured from Promega Corp (USA). Routine laboratory buffers, solvents, and salts were either from Merck (Mumbai, India) or SRL (Mumbai, India).
7
Cell Culture Maintenance and Characterization
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The THP1 Dual™ and A549 Dual™ cells were procured from InvivoGen having two inducible reporter constructs, for nuclear factor kappa B (NF-κ B) and interferon-stimulated response element (ISRE). THP1 Dual™ cells were maintained in RPMI-1640 (cell clone), 10% FBS (fetal bovine serum) (Gibco), penicillin and streptomycin (100 U/mL) (HiMedia), and the addition of selective antibiotics (Zeocin 100 µg/mL and Blasticidin 10 µg/mL) (InvivoGen). PBMCs were procured from HiMedia (#CL027). MDA-MB-231 (breast cancer) and A549 Dual™ (lung carcinoma) cell lines were cultured in high-glucose (cell clone) DMEM (Dulbecco’s Modified Eagle Medium) with 10% FBS and penicillin and streptomycin (100 U/mL). All cells were maintained at 37 °C and 5% CO2.
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