Xcelligence instrument

Manufactured by Roche

Sourced in Switzerland, Germany

The XCELLigence instrument is a label-free and real-time cell analysis system that measures cellular events, including cell attachment, spreading, proliferation, and death. The instrument utilizes electrical impedance to provide quantitative data on these cellular processes, enabling researchers to gain insights into cell behavior and dynamics.

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Lab products found in correlation

E plate, by Agilent Technologies (2 mentions) Cim plate, by Agilent Technologies (2 mentions) Resazurin, by Merck Group (1 mentions) Vi cell xr cell viability analyzer, by Beckman Coulter (1 mentions)

Spelling variants of this product

XCELLigence RTCA DP instrument (49 citations) XCELLigence Real-Time Cell Analyzer (RTCA) DP instrument (12 citations) XCELLigence RTCA instrument (7 citations) Real-Time Cell Analyzer (RTCA) Dual-Plate (DP) Instrument of the xCELLigence system (4 citations) XCELLigence RTCA MP Instrument (4 citations) XCELLigence Real-Time Cell Analysis (RTCA) DP instrument (4 citations) XCELLigence DP instrument (3 citations) XCELLigence System RTCA DP Instrument (3 citations) Roche xCELLigence Real-Time Cell Analyzer (RTCA) DP instrument (3 citations) XCELLigence® RTCA SP instrument (2 citations)

5 protocols using xcelligence instrument

Real-Time Cell Analysis of Tumor Cell Behaviors

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MC38-CT or MC38-CC1-L or MC38-CC1-FF cells were plated in a 16-well E-Plate or CIM-Plate (ACEA Biosciences; San Diego, California, USA) and proliferation, migration and invasion were measured using a real-time xCELLigence instrument (Roche; Basel, Switzerland). Fetal bovine serum (10%) was used as a chemoattractant in migration and invasion assays, with serum-free medium (SFM) as a negative control. Invasion assays were performed using Matrigel-coated CIM-Plates.

Arabzadeh A., Dupaul-Chicoine J., Breton V., Haftchenary S., Yumeen S., Turbide C., Saleh M., McGregor K., Greenwood C.M., Akavia U.D., Blumberg R.S., Gunning P.T, & Beauchemin N. (2015). Carcinoembryonic Antigen Cell Adhesion Molecule 1 long isoform modulates malignancy of poorly differentiated colon cancer cells. Gut, 65(5), 821-829.

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Cell Viability Assays: Impedance, Coulter, and Resazurin

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Cells were counted in real time with an xCELLigence instrument (Roche/ACEA Biosciences) monitoring impedance across gold microelectrodes. 8.5 × 10³ cells per well of a 96-well plate were seeded in 200 μl medium containing transfection reagents (hexaplicates per group). Medium and transfection reagents were refreshed after 48 h. For Coulter counting, cells were plated in six-well plates and transfected immediately after seeding with siRNA. After 96 h, cells (including supernatant) were harvested and counted in a Vi-CELL XR Cell Viability Analyzer (Beckman Coulter) (duplicates per group). For Resazurin assay, 3 × 10³ to 5 × 10³ cells per well of a 96-well plate were seeded in 100 μl medium containing the desired growth factor. After 72–96 h, Resazurin (Sigma-Aldrich) was added (20 μg/ml) and cells were incubated for another 2–6 h, depending on the cell line. Fluorescence signals proportional to the number of cells were recorded in a FLUOstar Omega plate reader (BMG labtech SARL).

Grünewald T.G., Bernard V., Gilardi-Hebenstreit P., Raynal V., Surdez D., Aynaud M.M., Mirabeau O., Cidre-Aranaz F., Tirode F., Zaidi S., Perot G., Jonker A.H., Lucchesi C., Le Deley M.C., Oberlin O., Marec-Bérard P., Véron A.S., Reynaud S., Lapouble E., Boeva V., Frio T.R., Alonso J., Bhatia S., Pierron G., Cancel-Tassin G., Cussenot O., Cox D.G., Morton L.M., Machiela M.J., Chanock S.J., Charnay P, & Delattre O. (2015). Chimeric EWSR1-FLI1 regulates the Ewing sarcoma susceptibility gene EGR2 via a GGAA microsatellite. Nature genetics, 47(9), 1073-1078.

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Real-time Proliferation, Migration, and Invasion Assays

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MC38-CT and -CC1-L cells were plated in 16-well E-Plates or CIM-Plates (ACEA Biosciences; San Diego, CA) and proliferation, migration and invasion were measured in real-time using an xCELLigence instrument (Roche; Basel, Switzerland) with assays performed in the presence of 1μM ALW-II-41-27 or NG-25 or DMSO. Fetal bovine serum (FBS; 10%) was used as chemoattractant in migration and invasion assays, with serum-free medium (SFM) as a negative control. Invasion assays were also performed using Matrigel-coated BD Falcon Cell Culture Inserts (BD Biosciences; Durham, NC) as described [71 (link)].

Arabzadeh A., McGregor K., Breton V., Van Der Kraak L., Akavia U.D., Greenwood C.M, & Beauchemin N. (2017). EphA2 signaling is impacted by carcinoembryonic antigen cell adhesion molecule 1-L expression in colorectal cancer liver metastasis in a cell context-dependent manner. Oncotarget, 8(61), 104330-104346.

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Real-time Proliferation Assay of ECs and VSMCs

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Real-time proliferation assay was performed using the xCELLigence instrument (Roche). For each 16-well E-plate (Agilent #5469830001), 3000 ECs and VSMCs from control and HGPS were seeded into each well, triplicated for each group, and incubated at 37°C. The impedance value of each well was monitored every 15 min for a total of 160 h and expressed as a Cell Index value. For counting cells within 10 days, 20 000 control-ECs and HGPS-ECs were seeded in 24-well plates and 20 000 control-VSMCs and HGPS-VSMCs were seeded in 12-well plates on Day 0. Cell number was counted every other day: Days 2, 4, 6, 8, and 10. Cell number and time curve were generated within 10 days.

Xu Q., Mojiri A., Boulahouache L., Morales E., Walther B.K, & Cooke J.P. (2022). Vascular senescence in progeria: role of endothelial dysfunction. European Heart Journal Open, 2(4), oeac047.

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Nanodiamonds Impact on Cell Growth

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Cells were seeded at a density of 2,000 cells/250 μL per well. Cell attachment was monitored using impedance measurement on an xCELLigence instrument (Roche, Germany). A stabilized impedance value indicated cell attachment (approximately 20 h post seeding). ND preconditioned media (2.5, 5, 10, 15 and 25 μg•mL -1 ) was added to wells and impedance measurements were performed every 15 min for 126 h. Impedance measurements were also conducted for 168 h. However, significant differences in cell membrane integrity were observed within 126 h of exposure. At day 7, cells were over confluent and started detaching from the electrodes. Thus, the results obtained only up to 126 h were more representative of true proliferation of the cells and were reported below.
Changes in electrical impedance were expressed as a dimensionless cell index value, obtained from the relative impedance changes corresponding to cellular coverage of the electrode sensors present in the wells. Before the cells were treated with nanodiamond, all impedance values were normalised to the values obtained from control cells (cells cultured in media only) at the time of exposure.

Khanal D., Zhang F., Song Y., Hau H., Gautam A., Yamaguchi S., Uertz J., Mills S., Kondyurin A., Knowles J.C., Knowles J.C., Georgiou G., Ramzan I., Cai W., Ng K.W, & Chrzanowski W. (2019). Biological impact of nanodiamond particles - label free, high-resolution methods for nanotoxicity assessment. Nanotoxicology, 13(9).

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