Au5431 automatic analyzer

In repeated oral dose toxicity study for 28 days, total protein, albumin, albumin/globulin (A/G), aspartate aminotransferase (AST), alanine aminotransferase (ALT), serum urea nitrogen (SUN), total lipid, phospholipid (PL), triglyceride (TG), total cholesterol, high-density lipoprotein cholesterol (HDL-C), and non-HDL-C were measured using an Olympus AU5431 automatic analyzer (Olympus Co., Tokyo, Japan) by a commercial service (Japan Medical Laboratory, Osaka, Japan). On the other hand, in repeated oral dose toxicity study for 13 weeks, in addition to the above-mentioned parameters, the hematological parameters including red blood cell (RBC) count, white blood cell (WBC) count, platelet (PLT) count, hemoglobin (Hb), hematocrit (Ht), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were also measured.

The serum TAG, PL, total cholesterol, high‐density lipoprotein cholesterol (HDL‐C), and non‐high‐density lipoprotein cholesterol (non‐HDL‐C) contents were measured with an Olympus AU5431 automatic analyzer (Olympus Co.) at Japan Medical Laboratory.
The liver total lipid was extracted using the method of Bligh and Dyer (1959). The liver TAG and PL contents were measured by Triglyceride E Test (Fujifilm Wako Pure Chemical Co.) and a phosphorus assay (Rouser et al., 1970). The cholesterol contents in the liver and epididymal WAT were analyzed by GC as described above. The main FA contents of the total lipid were also determined by GC as described above, and utilizing tridecanoic acid (C13:0‐FA), as an internal standard.
The fecal neutral sterol, including the cholesterol and coprostanol, contents were analyzed by GC, as described above (Kaneda et al., 1980). The fecal total bile acid (BA) content was analyzed with the total bile acid test kit (Fujifilm Wako Pure Chemical Co.), following the manufacturer's instructions. The fecal total sterol contents were calculated by adding the neutral sterols and the total BA contents.