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Anti nrf2 sc 13032

Manufactured by Santa Cruz Biotechnology
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Anti-NRF2 (sc-13032) is a primary antibody product offered by Santa Cruz Biotechnology. It is designed to detect NRF2 (Nuclear factor erythroid 2-related factor 2), a transcription factor that regulates the expression of antioxidant proteins.

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Lab products found in correlation

Mab1864, by Merck Group (1 mentions) Ne per nuclear and cytoplasmic extraction regents, by Thermo Fisher Scientific (1 mentions) Anti tubulin t9026, by Merck Group (1 mentions) Anti nrf1 d5b10, by Cell Signaling Technology (1 mentions) Anti o glcnac rl2 ab2739, by Abcam (1 mentions) Anti ogt sc 32921, by Santa Cruz Biotechnology (1 mentions) Anti hcf 1 a301 400a, by Fortis Life Sciences (1 mentions) Pvdf transfer membrane, by Merck Group (1 mentions) Collagenase type 4, by Worthington (1 mentions) Dual luciferase assay kit, by Promega (1 mentions)

Spelling variants of this product

Anti-Nrf2 (155 citations) Sc-13032 (17 citations) Nrf2 (sc-13032; (7 citations) Anti‐NRF2 antibody (sc‐13032) (2 citations)

3 protocols using anti nrf2 sc 13032

1

Quantifying Hepatic NRF2 Activation

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Mice were treated with CDDO-Me (30 μmol/kg BW) or vehicle (10% DMSO, 10% Cremophor-EL, and PBS) alone. Three hours after treatment, livers were collected to examine the nuclear translocation of NRF2. Nuclear extracts were prepared using NE-PER Nuclear and Cytoplasmic Extraction Regents (Thermo Scientific) according to the manufacturer’s directions. The protein samples were subjected to 8% SDS-PAGE and transferred to a polyvinylidene difluoride membrane. Specific protein signals were detected by anti-NRF2 (sc-13032, Santa Cruz Biotechnology Dallas, TX) and anti–α-tubulin (MAB1864, Millipore, Burlington, MA) antibodies. Image J (National Institutes of Health) was used for quantification analyses.
Gatbonton-Schwager T., Yagishita Y., Joshi T., Wakabayashi N., Srinivasan H., Suzuki T., Yamamoto M, & Kensler T.W. (2023). A Point Mutation at C151 of Keap1 of Mice Abrogates NRF2 Signaling, Cytoprotection in Vitro, and Hepatoprotection in Vivo by Bardoxolone Methyl (CDDO-Me). Molecular Pharmacology, 104(2), 51-61.
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2

Immunoblot Analysis of Protein Interactions

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Immunoblot analyses were performed as described previously (59 (link)). The antibodies that were used were anti-FLAG (F7425; Sigma), anti-OGT (sc-32921; Santa Cruz), anti-HCF-1 (A301-400A; Bethyl Lab), anti-NRF1 (D5B10; Cell Signaling), anti-O-GlcNAc/RL2 (ab2739; Abcam), anti-6×His (9C11; Wako), anti-GST (5A7; Wako), anti-β-TrCP (D13F10; Cell Signaling), anti-NRF2 (sc-13032; Santa Cruz), and antitubulin (T9026; Sigma).
Sekine H., Okazaki K., Kato K., Alam M.M., Shima H., Katsuoka F., Tsujita T., Suzuki N., Kobayashi A., Igarashi K., Yamamoto M, & Motohashi H. (2018). O-GlcNAcylation Signal Mediates Proteasome Inhibitor Resistance in Cancer Cells by Stabilizing NRF1. Molecular and Cellular Biology, 38(17), e00252-18.
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3

Nrf2-Keap1 Pathway Modulation

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All reagents were purchased from Sigma-Aldrich (St. Louis, MO) unless otherwise noted. Collagenase type IV was purchased from Worthington Biochemical Corporation (Lakewood, NJ). Anti-Nrf2 (sc-13032) and anti-Keap1 (sc-15246) were ordered from Santa Cruz Biotechnology (Santa Cruz, CA). Bortezomib was obtained from Millennium Pharmaceuticals (Cambridge, MA). PVDF transfer membrane was purchased from Millipore (Billerica, MA). Dual Luciferase Assay Kit was purchased from Promega (Madison, WI).
Smith E.J., Shay K.P., Thomas N.O., Butler J.A., Finlay L.F, & Hagen T.M. (2015). Age-related loss of hepatic Nrf2 protein homeostasis: potential role for heightened expression of miR-146a. Free radical biology & medicine, 89, 1184-1191.
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