Ey flp

Manufactured by BD

The Ey-FLP is a piece of laboratory equipment designed for specific biological applications. It functions as a tool for researchers, though its core purpose and features are not readily available in this context. A more detailed and unbiased description cannot be provided without access to additional information about the product.

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Lab products found in correlation

Uas dcr2, by BD (1 mentions) Uas gfpnls, by BD (1 mentions)

2 protocols using ey flp

Drosophila Genetic Tools for Optix Studies

Cited 2 times

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Drosophila lines were obtained from the Bloomington Drosophila Stock Center (BDSC) or the Vienna Drosophila RNAi Center (VDRC): UAS-Optix-RNAi (VDRC-KK110813), UAS-Dcr-2 (VDRC-60008), tsh[md621]-GAL4 (BDSC-3040), dpp[blk1]-GAL4 (BDSC-1553), UAS-Dcr-2; ey-FLP (BDSC-5580), UAS-GFP-nls (BDSC-4776), ato^5’EYE-GAL4 (Yu et al., 2015 (link)), the G-TRACE line UAS-FLP Ubi-p63E(FRT.STOP)Stinger (BDSC-28282; Evans et al., 2009 (link)) and Actin5C>y+>GAL4 (BDSC-3953); the latter two are referred to as UAS-FLP Ubi>IC>GFP and Act>IC>GAL4, respectively, in the text and figures (IC=interruption cassette). For reporter constructs, genomic DNA fragments were cloned into the vectors pCasper-β-gal (encoding cytoplasmic β-Galactosidase) or pStinger (encoding nuclear eGFP) (Pirrotta, 1988 (link); Barolo et al., 2000 ). Both vectors were first modified to contain a 1.1 kb fragment spanning the ato promoter region and 5’UTR (Sun et al., 1998 (link)). All constructs were confirmed by sequencing (data are available upon request) and injected into w CantonS for P-element transformation (Spradling and Rubin, 1982 (link)). All crosses for Fig. 4 were carried out at 30°C to enhance Optix gene silencing.

Zhou Q., DeSantis D.F., Friedrich M, & Pignoni F. (2016). Shared and distinct mechanisms of atonal regulation in Drosophila ocelli and compound eyes. Developmental biology, 418(1), 10-16.

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Fly Maintenance and Genetic Strains

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Flies were maintained on standard fly food at constant temperature 25°C under 12 h: 12 h light-dark cycles (12L: 12D). The following strains were obtained from the Bloomington stock center: y, w; hs-FLP, hs-I-SceI/CyO (BDSC #6934), y, w, ey-FLP (BDSC #5580) and y, w, Cre; +; D*/TM3, sb (BDSC #851). The isogenic iso31 wild type strain used for outcrossing and dysc s168 mutants were kind gifts from Kyunghee Koh (Thomas Jefferson University).
PDF::RFP was a kind gift from Dr Justin Blau (New York University), See Supplemental Information for further details of Materials and Methods.

Krätschmer P., Buhl E., Chen K., Lowe S.A., Kullmann D.M., Hodge J.J.L., & Cordivari C. (2020). A knock-in Drosophila model supports a conserved link between potassium channelopathy and involuntary movement.

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