Ai14 cre reporter mice

The following mouse lines (25-30 g, 8-12 weeks old, male) were used for the experiments: C57Bl6 mice (Jackson Laboratory), DAT::IRES-Cre (Jackson Laboratory, stock number: 006660, strain code: B6.SJL-Slc6a3tm1.1(cre)Bkmn/J), VGLUT2::IRES-Cre (Jackson Laboratory, stock number: 016963, strain code: Slc17a6tm2(cre)Lowl/J), VGLUT3::IRES-Cre (Jackson Laboratory, stock number: 018147, strain code: Tg(Slc17a8-icre)1Edw/SealJ), GAD2::IRES-Cre (Jackson Laboratory, stock number: 010802, strain code: Gad2^tm2(cre)Zjh/J), Ai14 Cre reporter mice (Jackson Laboratory, stock number: 007908, strain code: B6;129S6 Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J). Ai14 Cre reporter mice were crossed to GAD2::IRES-Cre and VGLUT2::IRES-Cre mice. Mice were maintained on a 12:12 light cycle (lights on at 07:00). All procedures complied with the animal care standards set forth by the National Institutes of Health and were approved by University of California Berkeley’s Administrative Panel on Laboratory Animal Care.

The following mouse lines (25–30 g, 8–12 weeks old, male) were used for the experiments: C57BL/6J mice (Jackson Laboratory), DAT-IRES-Cre (Jackson Laboratory, stock number: 006660, strain code: B6.SJL-Slc6a3^{tm1.1(cre)Bkmn}/J), VGLUT2-IRES-Cre (Jackson Laboratory, stock number: 016963, strain code: Slc17a6^tm2(cre)Lowl/J), GAD2-IRES-Cre (Jackson Laboratory, stock number: 010802, strain code: Gad2^tm2(cre)Zjh/J), VGAT-IRES-Cre (Jackson Laboratory, stock number:028862, strain code: B6J.129S6(FVB)-Slc32a1^tm2(cre)Lowl/MwarJ), Ai14 Cre reporter mice (Jackson Laboratory, stock number: 007908, strain code: B6;129S6 Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J). GAD2-Cre-tdTomato: Ai14 Cre reporter mice were crossed to GAD2-IRES-Cre mice. For ex vivo patch-clamp recordings shown in Figure 1g–m and Figure 2a–d male and female mice were used, but no significant differences were observed between male and female mice. Mice were maintained on a 12:12 light cycle (lights on at 07:00) with water and food ad libitum and room temperature of 22–25°C and 55% humidity. All procedures complied with the animal care standards set forth by the National Institutes of Health and were approved by University of California Berkeley’s Administrative Panel on Laboratory Animal Care.

The following mouse lines (25–30 g, 8–12 weeks old, males and females were counterbalanced across conditions with no significant effects of sex observed) were used for the experiments: C57Bl6 mice (Jackson Laboratory), D1-Cre (GENSAT, stock number: 017264-UCD, strain code: Tg(Drd1-cre)EY262Gsat/Mmucd), A2a-Cre (GENSAT, stock number: 017263-UCD, strain code: Tg(Drd2-cre)ER44Gsat/Mmucd), GAD2-IRES-Cre (Jackson Laboratory, stock number: 010802, strain code: Gad2^tm2(cre)Zjh/J), VGLUT2::IRES-Cre (Jackson Laboratory, stock number: 016963, strain code: Slc17a6tm2(cre)Lowl/J), Ai14 Cre reporter mice (Jackson Laboratory, stock number: 007908, strain code: B6;129S6 Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J). Ai14 Cre reporter mice were crossed to GAD2-IRES-Cre mice. Mice were maintained on a 12:12 light cycle (lights on at 07:00). All procedures complied with the animal care standards set forth by the National Institutes of Health and were approved by University of California Berkeley’s Administrative Panel on Laboratory Animal Care.