Xbp1000 system

Manufactured by Kent Scientific

The XBP1000 system is a laboratory equipment designed for analytical applications. It features high-precision measurements and reliable performance for various scientific and research purposes. The core function of the XBP1000 system is to provide accurate and consistent data acquisition and analysis capabilities to support laboratory workflows.

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Lab products found in correlation

Detectx serum creatinine kit, by Arbor Assays (3 mentions) Quantikine elisa kit, by R&D Systems (1 mentions) Polytetrafluoroethylene tube, by Braintree Scientific (1 mentions)

5 protocols using xbp1000 system

Comprehensive Biomarker Assessment in Animal Model

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Blood pressure (BP) was measured shortly before euthanasia by tail-cuff, using a XBP1000 system (Kent Scientific, Torrington, CT).^{12 (link)} Plasma cholesterol levels were determined at the Mayo Immunochemistry Laboratory using an automatic chemical analyzer with enzymatic assay kits. The level of plasma creatinine was assayed using the DetectX® Serum Creatinine kit (Arbor assays, Ann Arbor, MI).^{11 (link)} Standards or samples were pipetted into a microtiter plate with an assay diluent, and the color generating reaction was initiated with the DetectX® Creatinine Reagent. Urinary microalbumin was measured by the Mouse Microalbumin ELISA Kit (Kamiya Biomedical Company, Seattle, WA)^{13 (link)} according to the manufacturer’s instructions.

Kim S.R., Jiang K., Ogrodnik M., Chen X., Zhu X.Y., Lohmeier H., Ahmed L., Tang H., Tchkonia T., Hickson L.J., Kirkland J.L, & Lerman L.O. (2019). Increased Renal Cellular Senescence in Murine High-fat Diet: Effect of the Senolytic Drug Quercetin. Translational research : the journal of laboratory and clinical medicine, 213, 112-123.

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Systolic Blood Pressure and Kidney Biomarkers

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Systolic blood pressure (SBP) was measured biweekly by tail-cuff (XBP1000 system Kent Scientific, Torrington, CT) (Cheng et al., 2009 (link)). Plasma creatinine was assayed using the DetectX® Serum Creatinine kit (Arbor assays, Ann Arbor, MI)(Kim, Jiang, et al., 2019 (link)) and plasma activin-A by a Quantikine ELISA kit (R&D) (Kim, Puranik, et al., 2021 (link)).

Kim S.R., Jiang K., Chen X., Puranik A.S., Zhu X.Y., Lerman A., Tchkonia T., Kirkland J.L, & Lerman L.O. (2022). Selective kidney targeting increases the efficacy of mesenchymal stromal/stem cells for alleviation of murine stenotic-kidney senescence and damage. Journal of tissue engineering and regenerative medicine, 16(6), 550-558.

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Renal Artery Stenosis Induction in Mice

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To induce RAS, mice were anesthetized with 1.75% isoflurane supplemented with O₂ and placed prone on a heating pad at 37 °C. The right kidney was exposed by a flank incision and its renal artery bluntly dissected from the renal vein. A 0.15 mm (ID × 0.5 mm) long polytetrafluoroethylene tube (Braintree Scientific, Braintree, MA) cuff was placed around the right renal artery and tied with 10–0 nylon sutures. Kidneys were then returned to their original positions and the incisions sutured. Blood pressure was measured before RAS or Sham surgery (baseline) and at 2, 4, and 6 weeks after surgery by tail-cuff, using an XBP1000 system (Kent Scientific, Torrington, CT). Sham surgery consisted of isolation of the renal artery without placement of a cuff ^{61 (link)}. At day 3, 7, and 28 days post-RAS 4–6 mice were euthanized to assess expansion of macrophages.

Puranik A.S., Leaf I.A., Jensen M.A., Hedayat A.F., Saad A., Kim K.W., Saadalla A.M., Woollard J.R., Kashyap S., Textor S.C., Grande J.P., Lerman A., Simari R.D., Randolph G.J., Duffield J.S, & Lerman L.O. (2018). Kidney-resident macrophages promote a proangiogenic environment in the normal and chronically ischemic mouse kidney. Scientific Reports, 8, 13948.

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Telemetry Interference in MRI Experiments

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Telemetry transducers interfere with MRI. Therefore, using a XBP1000 system (Kent Scientific, Torrington, CT), systolic blood pressure (SBP) was measured by tail-cuff at baseline, 2, and 4 weeks. Plasma creatinine level and renin content were evaluated using the DetectX® Serum Creatinine kit (Arbor assays, Ann Arbor, MI) and the Angiotensin-I RIA kit (ALPCO, Salem, NH), respectively.

Chen X.J., Kim S.R., Jiang K., Ferguson C.M., Tang H., Zhu X., Lerman A., Eirin A, & Lerman L.O. (2021). Renovascular disease induces senescence in renal scattered tubular-like cells and impairs their reparative potency. Hypertension (Dallas, Tex. : 1979), 77(2), 507-518.

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Longitudinal Renal Fibrosis Progression

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This prospective study was approved by the Institutional Animal Care and Use Committee. Fifteen male 129S1 mice were used. At the age of 10 weeks all mice underwent a baseline MRI (K.J. and C.M.F., 6 and 3 years of experience in small-animal MRI, respectively), after which they were selected for RAS (n=10) or sham (n=5) surgeries (see Appendix) by an experienced technologist (H.T., 24 years of experience). To longitudinally monitor progression of fibrosis, follow-up MRI scans were performed (K.J. and C.M.F.) 2, 4 and 6 weeks post-surgery, intervals shown to induce progressive renal injury (25 (link)). Animals were weighed and blood pressure measured (H.T.) after MRI by tail-cuff (XBP1000 system, Kent Scientific, Torrington, CT).

Jiang K., Ferguson C.M., Ebrahimi B., Tang H., Kline T.L., Burningham T.A., Mishra P.K., Grande J.P., Macura S.I, & Lerman L.O. (2016). Noninvasive Assessment of Renal Fibrosis with Magnetization Transfer MR Imaging: Validation and Evaluation in Murine Renal Artery Stenosis. Radiology, 283(1), 77-86.

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