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5 protocols using tetrahydrofuran thf

1

Fabrication of Hybrid Protein-Polymer Materials

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Styrene (STY, >99.5%), divinylbenzene (DVB,
80%), 3-(trimethoxysilyl)propyl methacrylate (γ-MAPS, 98%),
2,2′-azobis(isobutyronitrile) (AIBN, 98%), n-decanol (99%), sodium hydroxide (NaOH), aluminum oxide, lysozyme
from chicken egg white (Lys), carbonic anhydrase from bovine heart
(CA, >90%), cytochrome c from equine heart (CC, >95%), bovine
casein
(Cas), and potassium iodide (KI, >99%) were purchased from Sigma-Aldrich
(St. Louis, Missouri, United States). Ethanol (EtOH), methanol (MeOH),
acetonitrile (ACN), and tetrahydrofuran (THF, >99.8) were purchased
from Biosolve (Valkenswaard, The Netherlands). Hydrochloric acid 37%
(HCl) and glacial acetic acid were obtained from Acros (Geel, Belgium).
Milli-Q water (18.2 MΩcm) was produced by a Sartorius Arium
611UV Ultrapure Water System (Göttingen, Germany). The titanium
devices were purchased from Materialise (Leuven, Belgium), while the
capillary (0.53 mm ID, 0.70 mm OD) was purchased from CMScientific
(Silsden, UK).
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2

Photocurable Liquid Crystal Coatings

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LC monomers 4-[[[4-[(1-Oxo-2-propenyl)oxy]butoxy]carbonyl]oxy]benzoic
acid 2-methyl-1,4-phenylene ester (LC242) and 4-Methoxybenzoic acid
4-(6-acryloyloxy-hexyloxy)phenyl ester (C6BP) were acquired from BASF
and Synthon, respectively. Photoinitiator camphorquinone (CQ) was
purchased from ABCR. Co-initiator ethyl 4-(Dimethylamino)benzoate
(EDAB) and photoinhibitor 2,2′-Bis(2-chlorophenyl)-4,4′,5,5′-tetraphenyl-1,2′-biimidazole
(o-Cl-HABI) were obtained from TCI. Chiral dopant
benzoic acid, 4-hexyl-,4-[[(1-methylheptyl)oxy]carbonyl]phenyl ester
(ZLI-811), was acquired from Merck.
Coatings used for cell preparation
included OPTMER AL 1254 from JSR (planar alignment), Sunever 5661
from Nissan Chemical (homeotropic alignment), 3-(trimethoxysilyl)propyl
methacrylate (adhesive coating), and trimethoxy(octadecyl)silane (antiadhesive
coating) from Sigma-Aldrich.
The developer solution comprised
isopropyl alcohol (IPA) from Biosolve,
propylene glycol monomethyl ether acetate (PGMEA) from Aldrich, and
methyl isobutyl ketone (MIBK) from Acros Organics. Dichloromethane
(DCM) and tetrahydrofuran (THF) were purchased from Biosolve.
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3

Synthesis of Ferrocene-Containing Polymer

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Poly(ferrocenyl(3-iodopropyl)methylsilane)
(PFS-I, Mw = 3.12 × 105 g/mol, Mn = 1.56 × 105 g/mol, Mw/Mn = 2.0) was prepared according to established procedures.31 (link) Dimethyl sulfoxide (DMSO) and tetrahydrofuran
(THF) were obtained from Biosolve. Tri-n-butylphosphine
(PBu3), dimethylaminopropyl methacrylamide (DMAPMA), 1-hexylamine,
and multi-walled carbon nanotubes (MWCNTs, 6–13 nm × 2.5–20
μm) were obtained from Aldrich and used without further purification.
Polyethylene glycol-tetrathiol (4-arm PEG-thiol, pentaerythritol core, Mn = 5000 g mol–1) was purchased
from JenKem Technology, USA. Sodium 1-pentanesulfonate (NaC5S) and sodium 1-hexanesulfonate (NaC6S) were supplied
by TCI, Belgium. Milli-Q water was used in all experiments.
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4

Sunitinib-Loaded Polymeric Nanoparticles

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Sunitinib malate was obtained from LC laboratories, USA. Poly(vinyl alcohol) (PVA; M w 30,000-70,000; 88% hydrolyzed), Tween 20, stannous octoate and dimethyl sulfoxide (DMSO) were obtained from Sigma Aldrich, Germany. Dichloromethane (DCM), acetonitrile and tetrahydrofuran (THF) were purchased from Biosolve (Valkenswaard, The Netherlands). PEG1000, 1,4-butanediol and 1,4 dioxane were purchased from Acros (Geel, Belgium), 1,4butanediisocyanate was purchased from Bayer (Leverkusen, Germany) and DL-lactide and L-lactide were purchased from Corbion Purac (Gorinchem, The Netherlands). Polystyrene standards were purchased from Agilent technologies (Santa Clara, USA). Fertilized white Leghorn eggs were obtained from Het Anker company (Ochten, The Netherlands).
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5

Formulation and Characterization of PLGA Microparticles

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PLGA (5004A: acid terminated with D,L-lactide/glycolide molar ratio 50:50, IV = 0.4 dl/g) was purchased from Purac, The Netherlands. Goserelin acetate was obtained from BCN (Barcelona, Spain). Polyvinyl alcohol (PVA; molecular weight 30,000-70,000; 88% hydrolyzed) was from Sigma-Aldrich, Inc., USA. Disodium hydrogen phosphate dihydrate (Na 2 HPO 4 Á2H 2 O) and sodium dihydrogen phosphate monohydrate (NaH 2 PO 4 ÁH 2 O) were obtained from Merck. Sodium azide (NaN 3 , Bio Ultra, P99.5%) was purchased from Sigma (Germany). HPLC and MS grade acetonitrile (ACN), peptide grade dichloromethane (DCM) and tetrahydrofuran (THF) were purchased from Biosolve (The Netherlands). Formic acid was purchased from Sigma-Aldrich Co (Zwijndrecht, the Netherlands).
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