80%), 3-(trimethoxysilyl)propyl methacrylate (γ-MAPS, 98%),
2,2′-azobis(isobutyronitrile) (AIBN, 98%), n-decanol (99%), sodium hydroxide (NaOH), aluminum oxide, lysozyme
from chicken egg white (Lys), carbonic anhydrase from bovine heart
(CA, >90%), cytochrome c from equine heart (CC, >95%), bovine
casein
(Cas), and potassium iodide (KI, >99%) were purchased from Sigma-Aldrich
(St. Louis, Missouri, United States). Ethanol (EtOH), methanol (MeOH),
acetonitrile (ACN), and tetrahydrofuran (THF, >99.8) were purchased
from Biosolve (Valkenswaard, The Netherlands). Hydrochloric acid 37%
(HCl) and glacial acetic acid were obtained from Acros (Geel, Belgium).
Milli-Q water (18.2 MΩcm) was produced by a Sartorius Arium
611UV Ultrapure Water System (Göttingen, Germany). The titanium
devices were purchased from Materialise (Leuven, Belgium), while the
capillary (0.53 mm ID, 0.70 mm OD) was purchased from CMScientific
(Silsden, UK).