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Hspa8

Manufactured by Cell Signaling Technology
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HSPA8 is a protein that functions as a molecular chaperone, facilitating the folding and transport of other proteins within the cell. It is involved in a variety of cellular processes, including protein trafficking, protein degradation, and the regulation of protein activity.

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Lab products found in correlation

Gapdh, by Cell Signaling Technology (2 mentions) Tsg101, by Abcam (1 mentions) Docetaxel, by Cayman Chemical (1 mentions) Anxa2, by Cell Signaling Technology (1 mentions) Succinate assay kit, by Abcam (1 mentions) Hyou1, by Cell Signaling Technology (1 mentions) Hsp90ab1, by Cell Signaling Technology (1 mentions) Ki67 antibody, by Abcam (1 mentions) β actin antibody, by Merck Group (1 mentions) Enhanced chemiluminescent autoradiography, by Merck Group (1 mentions) Polyvinyl difluoride membrane, by Merck Group (1 mentions) Proteinase inhibitor cocktail, by MedChemExpress (1 mentions) Ai600 system, by GE Healthcare (1 mentions) Gapdh 60004 1 ig, by Proteintech (1 mentions) Irdye 800cw donkey anti rabbit, by LI COR (1 mentions) Mini protean tgx stain free gel, by Bio-Rad (1 mentions) Mjff2, by Abcam (1 mentions) Donkey anti mouse irdye 680lt, by LI COR (1 mentions) Mjf r21, by Abcam (1 mentions) Odyssey blocking buffer, by LI COR (1 mentions)

Close spelling variants of this product

Monoclonal rabbit HSPA8 Rabbit anti-HSPA8 HSPA8 antibody

4 protocols using hspa8

1

Exosome Proteomic Analysis Protocol

Cited 2 times
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Exosome pellets were lysed in lysis buffer (8 M Urea/2.5% SDS containing 5 μg.ml−1 leupeptin, 1 μg.ml−1 pepstatin, and 1 mM phenylmethylsulphonyl fluoride) as previously described [13 (link)]. The Bradford method was used for protein quantification and Western blot was conducted as previously reported [12 (link)]. The following primary antibodies were used: Alix, HSPA8, MET, and GAPDH (Cell Signaling Technology, Inc., MA, USA), Tsg101, CD63 (Abcam, CA, UK), and PTGR1 (Sigma-Aldrich, St. Louis). Blots were visualized with enhanced chemiluminescent agents (Forevergen biosciences, GZ, China).
Wang G., Liu W., Zou Y., Wang G., Deng Y., Luo J., Zhang Y., Li H., Zhang Q., Yang Y, & Chen G. (2019). Three isoforms of exosomal circPTGR1 promote hepatocellular carcinoma metastasis via the miR449a–MET pathway. EBioMedicine, 40, 432-445.
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2

Characterization of Prostate Cancer Cell Lines

Cited 3 times
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Prostate cancer PC-3 and 22RV1 cell lines, and benign prostate BPH1 line were obtained from ATCC (Manassas, VA) as described.16 (link),17 (link) C4–2 cell line was obtained from UroCor Inc (Oklahoma City, OK).18 (link) All cell lines were tested for authentication at Genetica DNA Lab (Burlington, NC) and were used within passages of 15–25. Cells were cultured in RPMI 1640 media containing 10% fetal bovine serum and 1% penicillin/streptomycin in a 5% CO2-humidified atmosphere at 37°C. Biotin labeling of Alternol was carried out in house. Succinate assay kit and enzymatic function assay kits for PDHC, KGDHC, FH, and MDH2 were obtained from BioVision (Milpitas, CA). Docetaxel was obtained from Cayman Chemical (Ann Arbor, MI). Alternol was obtained from Sungen Bioscience as reported14 (link) (Shantou, China).
Antibodies for Caspase-3, DLAT, DLST, FH, MDH2, GAPDH, HSPD1, HYOU1, HSP90AB1, HSPA8, ILF2, NPM1, PDIA6, ATP5A1, ANXA2, TUBA1B, and LAMR1 were obtained from Cell Signaling Technology (Danvers, MA). TUNEL immunostaining kit, ETC complex proteins antibodies and Ki-67 antibodies were obtained from Abcam (Cambridge, MA). Beta-actin antibody was obtained from Sigma (St. Louis, MO). Streptavidin-agarose beads and secondary antibodies were obtained from Santa Cruz Biotech (Santa Cruz, CA).
Li C., He C., Xu Y., Xu H., Tang Y., Chavan H., Duan S., Artigues A., Laird Forrest M., Krishnamurthy P., Han S., Holzbeierlein J.M, & Li B. (2019). Alternol eliminates excessive ATP production by disturbing Krebs cycle in prostate cancer. The Prostate, 79(6), 628-639.
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3

Western Blot Protocol for Protein Analysis

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Cells were lysed in radioimmunoprecipitation lysis buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 1% NP-40, 0.1% sodium dodecyl sulfate (SDS), 0.1% sodium deoxycholate) containing proteinase inhibitor cocktail (MedChemExpress, Monmouth Junction, NJ, USA). The protein samples were electrophoresed through 10% SDS polyacrylamide gels and transferred onto polyvinyl difluoride membranes (Millipore, Billerica, MA, USA). After blocking with 5% bovine serum albumin at room temperature for 1 h, the membranes were incubated with primary antibodies at 4 °C overnight, and then incubated with secondary antibodies at room temperature for 1 h. Immunoreactivity was detected with enhanced chemiluminescent autoradiography (Millipore). Chemiluminescence was determined using the AI600 System (GE Healthcare, Little Chalfont, Buckinghamshire, UK). The antibody against GAPDH (60004-1-Ig, dilution 1:1000) was purchased from Proteintech. Antibodies against HSPA8 (8444, dilution 1:1000) and DEK (29812, dilution 1:1000) were purchased from Cell Signaling Technology (Danvers, MA, USA).
Yang C., Shao Y., Wang X., Wang J., Wang P., Huang C., Wang W, & Wang J. (2023). The Effect of the Histone Chaperones HSPA8 and DEK on Tumor Immunity in Hepatocellular Carcinoma. International Journal of Molecular Sciences, 24(3), 2653.
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4

LRRK2 and Rab10 Phosphorylation Assay

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Protein lysates were electrophoresed on a 7.5% Mini-Protean TGX stain- free gel (BioRad) and then transferred onto Immobilon-FL membranes (Millipore) and blocked with Odyssey Blocking Buffer (LiCOR). Membranes were cut in half below the high-molecular weight markers. Primary antibodies included mouse anti-LRRK2 clone N241A/34 (1: 2000, Antibodies, Inc.), rabbit anti-LRRK2 antibody MJFF2 (1:2000, Abcam), Ser(P)-935 (1: 2000, Abcam), Ser(P)-1292 (1:2000, Abcam) HSPA8 (1:5000, Cell Signaling), phospho-T73-Rab10 antibody (1:1000, MJF-R21, Abcam), and total Rab10 (1:1000, clone D36C4, Cell Signaling). Secondary antibodies were IRdye 680LT donkey anti-mouse and IRdye 800CW donkey anti-rabbit (both 1:10000, LiCOR). Membranes were scanned using a LiCOR CLx with 685-nm and 785-nm lasers and band intensities were quantified using the LiCOR-Odyssey system.
Kelly K., Wang S., Boddu R., Liu Z., Moukha-Chafiq O., Augelli-Szafran C, & West A.B. (2018). The G2019S Mutation in LRRK2 Imparts Resiliency to Kinase Inhibition. Experimental neurology, 309, 1-13.
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