The paraffin sections (4 µm thickness) were cut transversely via the lesion area. After deparaffinizing and antigen retrieving, the section was exposed to primary antibody against CD31 (1:200, Abcam, Cambridge, UK) overnight at 4 °C. Next, the section was exposed to secondary antibodies (anti-rabbit IgG antibodies) for 1 h. Lastly, the section was stained with diaminobenzidine (DAB) for visualization and was recorded with optical microscopy (Olympus). The Image-Pro Plus 6.0 was employed to count the positive cells.
Primary antibody against cd31
Manufactured by Abcam
Sourced in United Kingdom
The primary antibody against CD31 is a laboratory reagent used to detect and identify the CD31 protein, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1). CD31 is a cell surface glycoprotein expressed by endothelial cells and is involved in cell-cell adhesion and signaling.
Automatically generated - may contain errors
2 protocols using primary antibody against cd31
1
Immunohistochemical Analysis of CD31
2
Quantifying Angiogenesis in Cryo-sectioned Tumors
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cryo-sectioned tumor tissues (5 µm) were incubated overnight with a primary antibody against CD31 (1:200, Abcam). The sections were then incubated with an anti-rabbit secondary biotinylated antibody (1:1000, Vector Laboratories) and visualized with streptavidin conjugated to fluorescein (Vector Laboratories). Fluorescence was measured with a confocal laser scanning microscope (Leica).
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