Four-week-old female nude mice were purchased from Vital River Company (Beijing, China) and maintained in Specific Pathogen Free (SPF) animal facility (68–71.6 °F temperature and 50–60% humidity). 8 × 105 MGC803 cells constitutively expressing luciferase cells in 0.2 ml PBS were injected into the tail vein of mice. Ten days later, pentobarbital-anesthetized mice were injected intraperitoneally with the d -luciferin substrate (PerkinElmer, USA; 15 mg/ml in PBS) and imaged under a Bruker In-Vivo Xtreme system to observe the metastasis. For all analyses, an additional ROI was employed to normalize for background luminescence on each image. After the metastasis model was established in most mice, they were randomly divided into three groups (6 mice per group). 14-5-18 was dissolved in a mixed solvent of DMSO (2%) and Tween 20 (5%) in water, and administrated by gavage at 0, 10, and 30 mg/kg for each group, once a day. After 18 days of administration, the mice were imaged with a Bruker In-Vivo Xtreme system. Mice were sacrificed, and their lungs were removed and stained by Hematoxylin and Eosin (H & E) staining.
In vivo xtreme system
Manufactured by Bruker
Sourced in United States
The In-Vivo Xtreme system is a preclinical imaging platform designed for small animal research. It provides high-resolution imaging capabilities to capture and analyze biological functions and anatomical details.
Automatically generated - may contain errors
10 protocols using in vivo xtreme system
1
Metastasis Inhibition by 14-5-18 in Nude Mice
2
Bioluminescent Tumor Imaging in Mice
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The d -luciferin potassium salt was purchased from Solarbio (D8930; Beijing Solarbio Science & Technology Limited Company, Beijing, China). The absorption time of the drug is 10–15 min, and radioactivity and cell survival cannot be detected. Before observation, 15 mg/mL luciferin potassium solution was injected into each mouse intraperitoneally at a concentration of 10 mg/g body mass. After 5 min, the mice were injected with 1% pentobarbital sodium (50 mg/kg) intraperitoneally according to body weight, and tumor growth was observed by in vivo imaging using the In-Vivo Xtreme system (Xtreme BUX00081; Bruker Corporation, Billerica, MA, USA).
3
Non-Invasive Bioluminescence Imaging
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The mice were again anesthetized with 2% isoflurane and oxygen. In experiments in which AAV-fLuc was used, the d-luciferin substrate (Biotium, Hayward, CA) was injected intraperitoneally at a dose of 150 μg/g of body weight. In contrast, when AAV-gLuc was used, either 100 μg of standard coelenterazine or 100 μg of coelenterazine-SOL (Nanolight, Pinetop, AZ) was injected intraperitoneally. The mice were then placed in a dark chamber inside of a Bruker In Vivo Xtreme system (Billerica, Massachusetts). Images were generated using a cryogenically cooled charge-coupling device camera IVIS 100 (Xenogen, Alameda, CA). For each mouse, bioluminescence images were taken at either 20 min (fLuc) or 1 min (gLuc) following substrate injection. Both light and dark images of mice were collected, and the dark images were then pseudocolored using the Bruker software (Molecular Imaging software v7.5). Visual output was set to represent the number of photons emitted/second/cm2 as a false color image, where maximum intensity is colored white and the minimum is colored violet. All animals were imaged on a schedule of 3, 7, 14, 21, 28, 35, 42, and 56 days following injection of AAV into the caudal IVD.
4
In Vivo Bioluminescence Imaging in Mice
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Mice were intraperitoneally administered 150 mg/kg D-Luciferin (Santa Cruz Biotechnology) 10 min before imaging. Animals were anesthetized with 2% isoflurane, and bioluminescent signal was captured using the In-vivo Xtreme system (Bruker). Mice were imaged weekly starting from 6 weeks after the transfection.
5
Angiogenesis Imaging Using AngioSense 750EX
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The AngioSense 750EX Fluorescent Imaging Agent (Perkin Elmer) was injected into the mouse model of GC cell xenografts by tail vein (2 nmol/100 μl per mouse). After 4 h, the probe had accumulated in tumors and enabled imaging of vascularity, perfusion, and vascular permeability at a wavelength of 750 nm. Angiogenesis fluorescence imaging in vivo was detected with an In-Vivo Xtreme system (Bruker, Baden-Wurttemberg, Germany) and quantified by measuring the tumor uptake of the probe AngioSense 750EX.
6
Evaluating Therapeutic Strategies for Chemoresistant Cancers
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Briefly, SCC‐25, SCC‐25/sh‐con, SCC‐25/sh‐XBP1 and SCC‐25/sh‐KAT6B cells were treated with 8 µg/ml cDDP for 24 h. Then, SCC‐25/Luc cells alone or mixed with cDDP‐treated SCC‐25 cells, SCC‐25/Luc cells mixed with cDDP‐treated SCC‐25/sh‐con or SCC25/sh‐XBP1 cells, SCC‐25/Luc/Con or SCC‐25/Luc/IκBα mixed with cDDP‐treated SCC‐25 cells and SCC‐25/Luc cells mixed with lethally cDDP‐treated SCC‐25/sh‐con or SCC25/sh‐KAT6B cells were subcutaneously injected into BALB/C athymic nude mice (4 weeks, Guangdong Medical Laboratory Animal Center, China) to generate xenograft tumours. The mice were pretreated with MK2206 (oral gavage, 120 mg/kg) or phosphate‐buffered saline (PBS) for 2 days, SCC‐25/Luc cells mixed with cDDP‐treated SCC‐25 cells were subcutaneously injected, and then, the mice were treated with MK2206 (oral gavage, 120 mg/kg) or PBS three times within 1 week for 4 weeks. The tumour growth was measured every 5 days. At the experimental end points, bioluminescent imaging was performed using an In‐Vivo Xtreme system (Bruker). Ten minutes before imaging, the mice were intraperitoneally injected with D‐luciferin (150 mg/kg, GoldBio, China). The animal studies were approved by the Institutional Animal Care and Use Committee (IACUC) of Guangzhou Medical University (GY2014‐057). Laboratory guidelines and animal care were in accordance with the IACUC protocol.
7
Radiographic Analysis of Aged Murine Bones
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Control and treated male mice at 8 months of age were anesthetized using isoflurane (induction: 3 l/min in pure O2; maintenance: 1.5 l/min in pure O2) and radiographed (45 keV) using the Bruker In‐Vivo Xtreme system. Due to cost constraints and obvious skeletal changes, only 5–11 mice per group were x‐rayed. X‐ray images were analyzed using ImageJ software for individual bones widths.
8
In Vivo Tracking of Labeled BMDCs
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Cell-tracker CM-DIL (Invitrogen-C7000)-labeled BMDCs (5 × 106 BMDCs per mouse) were transferred into normal C57BL/6 mice by tail vein injection and were imaged in vivo with an In Vivo Xtreme system (Bruker, Billerica, MA, USA). The mice were euthanized at day 7 and day 14 after cell transfer, and the organs were collected and imaged by an In Vivo Xtreme system. On days 7 and 14, the spleens were harvested, fixed in 100% acetone and 1% paraformaldehyde, and stained with TUNEL (green) and BMDC-DIL (red); TUNEL staining represents apoptosis and was observed with a fluorescence microscope (ZEISS, Germany).
9
In vivo multimodal imaging of mice
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Optical in vivo imaging of mice was performed using the In-Vivo Xtreme system (Bruker, Billerica, MA, USA) equipped with a 400 watts xenon illuminator and a back-illuminated 4 megapixel charge-coupled device detector. For detection of mCherry, fluorescence imaging (FLI) was performed for 5 s at λEx/Em = 600/700 nm. Reference images were captured at λEx/Em = 480/535 nm. Photostimulated luminescence imaging (PLI) was performed for 2 minutes at 4 × 4 binning. X-ray imaging, was performed for 1.2 s. Images were analyzed using the MI 7.2 software (Bruker). For demarcation of tumors, regions of interest were determined in mCherry/reference-divided fluorescence images by thresholding (signal/background = 2). For demarcation of muscles, regions of interest were determined manually in X-ray images at the position of the masseters. Accumulation of radioligands was determined in PLI images as the maximum uptake value (max UVPLI = [photons/s/mm2] / [MBq injected activity/g body weight]).
10
In vivo Bioluminescence Imaging Procedure
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BLI was performed using the In vivo Xtreme system (Bruker, Billerica, MA, USA). d- Luciferin was prepared and administered according to the manufacturer’s instructions (Caliper Life Sciences, Hopkinton, MA, USA). Images were analyzed using the MI 7.2 software (Bruker). In vitro, net luminescence intensity (photons/s/mm2) of serially diluted tumor cells was determined in a 96-well microplate (n = 2). In vivo, net luminescence intensities were determined from dynamic image series. Signals from successfully induced subcutaneous tumors in NMRI-nude mice (n = 10) were quantified from sagittal view images. Signals from successfully induced metastases in NMRI-nude (n = 7), NK cell-depleted NMRI-nude (n = 5), SHO (n = 6), SCID/beige (n = 9) and SKH1 mice (n = 12) were quantified by summating the intensities of ventral and dorsal view images. X-ray images were merged with bioluminescence images using the linear dodge blending mode of Photoshop CS5 (Adobe).
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