Xad 16 adsorber resin

All myxobacterial cultures were grown in 300 mL shake flasks containing 50 mL of VY/2, VY, PYGS, or CyHv3 medium (Tables S1–S4) for both Corallococcus sp. MCy9049 and Myxococcaceae sp. MCy9003. Following inoculation with 1 mL pre-culture the medium was supplemented with 2% of sterile XAD-16 adsorber resin (Sigma Aldrich, Taufkirchen, Germany) suspension in water to bind metabolites from the culture medium. Small scale cultures were grown for 10–12 days until the fermentation medium had cleared up except for the myxobacterial biofilm clumps and XAD-16 resin particles. After fermentation the cultures were pelleted in a 50 mL tubes at 6000 rcf for 10 min using a table centrifuge (Eppendorf) and stored at −20 °C until further use. The myxobacterial strains were kept in agar culture for storage for timespans of a few days. The agar media used in this case were VY/2 agar, prepared by adding 14 g/L agarose (BD) to VY/2 medium before autoclaving.

Cultures for analytical purposes were grown in 300 mL shake flasks containing 50 or 100 mL of the respective fermentation medium inoculated with 2% (vol/vol) pre-culture. The medium was supplemented with 4% (vol/vol) of a sterile aqueous solution of XAD-16 adsorber resin (Sigma Aldrich) to bind secondary metabolites in the culture medium. After 7–10 days of cultivation, the culture is pelleted in 50 mL falcon tubes in an Eppendorf centrifuge 5804R at 8,288 × g and 4°C for 10 min. The pellet is then stored at −20°C until further use.

P. pacifica DSM 14875^T was cultivated at 30 °C in 13.2L RG224 medium in Erlenmeyer flasks (six 5 L flasks each with a volume of 2 L medium and 200 mL pre-culture) for the isolation procedure of 1–8. Each Erlenmeyer flask was inoculated with pre-culture inoculum (200 mL) in the same medium. After inoculation, the medium was supplemented with 2% (v/v) of sterile XAD-16 adsorber resin (Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany) suspension in water to bind secondary metabolites in the culture medium. The cultures were shaken on a rotary shaker at 180 rpm for 14 days at 30 °C. Myxobacterial cells and adsorber resin were harvested together by centrifugation after fermentation (4 °C, 8000 rpm, 30 min), whereas the supernatant was discarded.