Objective n a 0

Manufactured by Nikon

Sourced in United States

The 10x objective (N.A. 0.45) is a standard optical lens component used in various laboratory equipment. It provides a 10x magnification with a numerical aperture of 0.45, which determines the light-gathering capability of the lens. This objective is designed to be part of a larger optical system and serves to magnify and focus the image of a specimen or sample.

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Lab products found in correlation

Motorizedxy stage, by Nikon (1 mentions) Mathematica software, by Wolfram (1 mentions) Ds qi2, by Nikon (1 mentions) Eclipse ti, by Nikon (1 mentions) Eclipse ti e, by Nikon (1 mentions)

Spelling variants of this product

CFI plan fluor 10 × /0.30 N.A. objective lens (4 citations)

2 protocols using objective n a 0

Microfluidic Droplet Imaging and miRNA Analysis

Cited 1 time

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Following incubation,
emulsions were imaged by microscopy. A monolayer of droplets was sandwiched
between two glass slides (1 mm thick bottom slide, Paul Marienfeld
GmbH & Co, 0.17 mm thick top slide, VWR) spaced using 10 μm
polystyrene particles (Polysciences, Inc.) to avoid droplet compression.
The chamber was sealed with epoxy glue (Sader). Images were acquired
on an epifluorescence microscope Eclipse Ti equipped with a motorized
XY stage (Nikon), a camera Nikon DS-Qi2, an apochromatic 10×
objective (N.A. 0.45, Nikon) and a CoolLed pE-400 illumination source.
Composite images were generated with the open source software ImageJ.
Droplets were analyzed using the Mathematica software (Wolfram) by
following a previously reported procedure.^{28 (link)} The concentration of microRNA is computed using the formula and where F_g and F_r are the
fraction of green- and red-positive
droplets, respectively; N_A is the Avogadro
number, and V is the volume of the droplets.

Rondelez Y, & Gines G. (2020). Multiplex Digital MicroRNA Detection Using Cross-Inhibitory DNA Circuits. ACS Sensors, 5(8), 2430-2437.

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Fluorescence Microscopy Quantification

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Images were taken with an inverted Eclipse Ti-E (Nikon Instruments Inc., Melville, NY, USA) microscope equipped with a 10× objective (N.A. 0.45, Nikon Instruments Inc., Melville, NY, USA) and an EMCCD camera (iXon Ultra, Andor-an Oxford Instruments company, Belfast, Ireland). ANDOR iQ3 Imaging software (Andor-an Oxford Instruments company, Belfast, Ireland) was used for stage movement control and image capture. Quantification of fluorescence intensity and number of cells was done with NIH ImageJ.

Nemcovsky Amar D., Epshtein M, & Korin N. (2019). Endothelial Cell Activation in an Embolic Ischemia-Reperfusion Injury Microfluidic Model. Micromachines, 10(12), 857.

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