The largest database of trusted experimental protocols

Jem 1010

Manufactured by Olympus
Sourced in Japan, Germany

The JEM-1010 is a compact and versatile transmission electron microscope (TEM) designed for a wide range of applications. It offers high-resolution imaging capabilities and supports various sample preparation techniques.

Automatically generated - may contain errors

2 protocols using jem 1010

1

Histological Analysis of Kidney Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
After the kidneys were removed, the tissues were decapsulated and fixed in 4% paraformaldehyde and glutaraldehyde. After 48 h, fixed tissues were embedded in paraffin for observation of the pathological changes under light microscopy (Olympus, Japan) or embedded in 1% lanthanum nitrate for ultramicrostructural observation of the podocytes and glomerular basement membrane (GBM) under electron microscopy (JEM-1010, Japan). The tissues were cut into 2-μm-thick slices and then stained with periodic acid-Schiff (PAS) solution and wheat germ agglutinin (WGA) after removing the paraffin. Immunofluorescent staining of the tissue slices was performed using primary antibodies against Wilms’ tumor 1 (WT-1) and nephrin (Santa Cruz, USA) and examined by laser confocal microscopy (Leica, Germany).
+ Open protocol
+ Expand
2

Characterization of Extracellular Vesicles by TEM

Check if the same lab product or an alternative is used in the 5 most similar protocols
EVs were phenotypically and structurally characterized by Transmission Electron Microscopy (TEM) with immuno-gold labelling against CD9, as previously described by Nielsen et al [44 (link)]. Briefly, 5 μl of EV isolate was mounted on a grid (SPI Supplies, PA, USA) and stained with one drop of 1% (w/v) phosphotungstic acid (pH 7.0, Ted Pella, Caspilor AB, Sweden), and subsequently blotted dry on filter paper. To visualize the presence of EV-specific marker CD9 on the surface of vesicles, IEM was performed on the isolated vesicles. The pelleted vesicles were positioned on a grid as described above and then blocked in ovalbumin. Subsequently, the grid was incubated with primary anti-CD9 antibody (1:50, BD Pharmingen, CA, USA), followed by incubation with secondary goat anti-mouse antibody conjugated with 10 nm colloidal gold (1:25, British BioCell, UK). The grids were stained with 1% (w/v) phosphotungstic acid at pH 7.0 and blotted dry. Images were obtained with a transmission electron microscopy (JEM 1010, Germany) operated at 60 keV coupled to an electron-sensitive CCD camera (KeenView, Olympus, PA, USA). Lastly, a grid-size replica (2,160 lines/mm) was imported to ImageJ 1.50i software, which enables a correct determination of the size of EVs.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!