Chloroform (CHCl3) and ethyl acetate (EA) were purchased from Macron Fine Chemicals (Center Valley, PA, U.S.A.). Formic acid and methanol were obtained from Sigma–Aldrich(St. Louis, MO, U.S.A.) and Merck (Darmstadt, Germany), respectively. Ethanol and acetic acid were purchased from J.T. Baker (Phillipsburg, NJ, U.S.A.). Distilled deionized water was produced by a PURELAB purifier (ELGA, Marlow, U.K.). Standards for illegal drugs including 5-MeO-AMT, amphetamine (AM), methamphetamine (MA), mephedrone, para-methoxymethamphetamine (PMMA), 3,4-methylenedioxymethamphetamine (MDMA), methylone, 2-(4-chloro-2,5-dimethoxyphenyl)ethan-1-amine (2C-C), ketamine, nitrazepam, flunitrazepam (FM2), and lysergic acid diethylamide (LSD) were purchased from Cerilliant Corporation (Round Rock, TX, U.S.A.). Stock solutions of illicit drugs were prepared in MeOH. Drinks and powders for whole fat milk (Uni-President Enterprises Corporation), grape juice (Uni-President Enterprises Corporation), fruit and vegetable juice (Chou-Chin Industrial Co.), sugar-free green tea bags (Vitalon Foods Company), 3-in-1 instant coffee packs (Nestlé Company), matcha tea packs (Starbucks), and gelatin powder (Tehmag Foods Corporation) were obtained from local supermarkets. Postage stamps were purchased from Chunghwa Post Co., Taiwan.
Mephedrone
Manufactured by Cerilliant
Sourced in United States
Mephedrone is a chemical compound used in laboratory research. It has a molecular formula of C11H15NO and is classified as a stimulant. The core function of Mephedrone is to serve as an analytical reference standard for the purpose of identification and quantification in chemical analysis.
Automatically generated - may contain errors
Lab products found in correlation
Methylone, by Cerilliant (3 mentions)
Ketamine, by Cerilliant (3 mentions)
3 4 methylenedioxymethamphetamine, by Cerilliant (3 mentions)
Amphetamine, by Cerilliant (3 mentions)
Methamphetamine, by Cerilliant (3 mentions)
Norketamine, by Cerilliant (2 mentions)
7 aminonimetazepam, by Cerilliant (2 mentions)
Codeine, by Cerilliant (2 mentions)
Morphine, by Cerilliant (2 mentions)
Methylone d3, by Cerilliant (2 mentions)
Methcathinone, by Cerilliant (2 mentions)
Mephedrone d3, by Cerilliant (2 mentions)
Methanol, by Merck Group (1 mentions)
Nitrazepam, by Cerilliant (1 mentions)
Formic acid, by Merck Group (1 mentions)
Para methoxymethamphetamine, by Cerilliant (1 mentions)
Milli q ultrapure water system, by Merck Group (1 mentions)
Benzoylecgonine, by Cerilliant (1 mentions)
Codeine d6, by Cerilliant (1 mentions)
Morphine d6, by Cerilliant (1 mentions)
6 protocols using mephedrone
1
Detection and Quantification of Illicit Drugs
2
Quantification of Illicit Drugs in Cough Syrups
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Carbinoxamine, chlorpheniramine, and chlorpheniramine-d6 were purchased from TRC (North York, Canada). Individual solutions of 6-AM, 7-aminonitrazepam, 7-aminonimetazepam, amphetamine, benzoylecgonine, C6G, codeine, codeine-d6, ketamine, methamphetamine, mephedrone, 4-methylephedrine, 3,4-methylenedioxymethamphetamine hydrochloride, M3G, M6G, morphine, morphine-d6, and norketamine (each with a concentration of 1 mg mL−1) were purchased from Cerilliant (Round Rock, TX, USA). Three brands of cold syrups (Consrine, ssBuron, and Pabron) manufactured in Taiwan were purchased from local pharmacies. Consrine contains codeine phosphate (0.48 mg mL−1), chlorpheniramine (0.25 mg mL−1), dl-methyl ephedrine HCl (1.6 mg mL−1), and glycyrrhizic acid (0.25 mg mL−1), ssBuron contains codeine phosphate (0.5 mg mL−1), guaifenesin (3.33 mg mL−1), chlorpheniramine maleate (0.14 mg mL−1), and caffeine (1.33 mg mL−1), Pabron contains codeine phosphate (0.48 mg mL−1), dl-methyl ephedrine HCl (2.5 mg mL−1), and carbinoxamine maleate (0.4 mg mL−1). Formic acid and methanol (high-performance LC grade) (≥ 98%) were purchased from J.T. Baker (Pittsburgh, PA, USA). Ultrapure water (18.2 mΩ·cm) obtained from a Milli-Q ultrapure water system (Millipore, Billerica, MA, USA) was used in this study.
3
Quantitative Analysis of Illicit Drugs and Novel Psychoactive Substances
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LC–MS-grade methanol (purity, ≥99.9%) was purchased from Merk (Darmstadt, Germany). HPLC-grade acetonitrile (purity, ≥99.9%) was purchased from J.T. Baker (Phillipsburg, NJ, USA). Ten standards of illicit drugs, methamphetamine (1 mg/mL), amphetamine (1 mg/mL), ketamine (1 mg/mL), norketamine (1 mg/mL), morphine (0.1 mg/mL), codeine (1 mg/mL), Tetrahydrocannabinol (THC, 1 mg/mL), 11-nor-9-carboxy-THC (THC-COOH, 0.1 mg/mL), 3,4-methylenedioxymethamphetamine (MDMA, 1 mg/mL), mephedrone (1 mg/mL), flunitrazepam (1 mg/mL), 7-aminoflunitrazepam (1 mg/mL), nimetazepam (1 mg/mL), 7-aminonimetazepam (1 mg/mL), and zopiclone (1 mg/mL) were purchased from Cerilliant (Round Rock, TX, USA). Three standards of NPSs, 4′-chloro-alpha-pyrrolidinopropiophenone (4-Cl-α-PPP, 1 mg, purity, ≥98%), 4′-Fluoro-α-pyrrolidinopentiophenone (4-F-α-PVP, 1 mg, purity, ≥98%), and 1-pentyl-3-(2-bromophenylacetyl)indole (JWH-249, 1 mg, purity, ≥98%) were purchased from Cayman Chemicals (Ann Arbor, MI, USA). Sodium azide (500 mg, purity, ≥99%) was purchased from Sigma-Aldrich (St. Louis, MO, USA).
4
Drug Quantification with Mass Spectrometry
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Drug checking samples were provided by the harm reduction association “Nuit Blanche.” LSD, methamphetamine, MDEA, MDMA, amphetamine, mephedrone, and cocaine, as well as their deuterated analogs, were purchased as standards at 1 mg/ml either from Cerilliant or Lipomed. Acetonitrile (ULC-MS 99.99%), methanol (ULC-MS 99.99%), water (ULC-MS), formic acid 99% (ULC-MS), trifluoroacetic acid, as well as the ammonium formate salt were purchased from Biolsolve. Alpha-Cyano-4-hydroxycinnamic acid (CHCA) was purchased from Sigma-Aldrich.
5
Comprehensive Synthetic Cathinone Analysis
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The stock standard solutions of amphetamine (A), methamphetamine (MA), phenylpropanolamine (PHPR), ephedrine, pseudoephedrine (PSE), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxy-N-ethylamphetamine (MDEA), phentermine (PHRM), methylphenidate (MPHD), bupropion, hydroxybupropion, methcathinone (ephedrone), methedrone (PMMC), mephedrone (4-MMC), methylone (MDMC), ethylone (MDEC), 3,4-methylenedioxypyrovalerone (MDPV), and alpha-pyrrolidinopentiophenone (alpha-PVP) at a concentration of 1.0 mg mL -1 for each analyte in methanol were purchased from Cerilliant (Round Rock, Texas, USA). Stock standard solutions of the internal standards (ISTDs), namely amphetamine-d11, methamphetamine-d11, ephedrine-d3, pseudoephedrine-d3, norpseudoephedrine-d3, MDA-d5, MDMA-d5, MDEA-d5, phentermine-d5, methylphenidate-d9, bupropion-d9, hydroxybupropion-d6, mephedrone-d3, and methylone-d3, were also purchased from Cerilliant (Round Rock, TX, USA) at a concentration of 100 µg mL -1 in methanol.
6
Quantification of Novel Psychoactive Substances
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Cathinone, methcathinone, methylone, N-ethylcathinone, buphedrone, mephedrone, 4-methylethcathinone, α-pyrolidinovarophenone (PVP), MDPV, and naphyrone (1 mg/mL), and internal standards MDPV-d8, mephedrone-d3, methylone-d3, and naphyrone-d5 (100 µg/mL) were obtained from Cerilliant (Round Rock, TX, USA). Solid phase extraction (SPE) cation exchange cartridges Strata Drug-X B 60 mg/3 mL were from Phenomenex (Torrance, CA, USA). Glacial acetic acid, acetonitrile, ammonium hydroxide, and formic acid were acquired from Pharmco-Aaper (Shelbyville, KY, USA). Methanol, dichloromethane, and isopropanol were acquired from Fisher Scientific (Pittsburgh, PA, USA). All solvents used in the extraction were high performance liquid chromatography (HPLC) grade and in the chromatographic instrument were liquid chromatography-mass spectrometry (LC-MS) grade. Quantisal buffer was obtained from Immunalysis Corp. (Pomona, CA, USA) and Oral-Eze buffer from Capitol Vial, Inc. (Auburn, AL, USA). Neat drug-free OF was obtained from healthy volunteers by spitting into a Corning® polypropylene 50 mL tube (Fisher Scientific).
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