Ab126

Antibodies used in this paper are listed as following: anti‐FEN1 antibody (42 282, Genetex), anti‐γH2AX antibody (ab26350, Abcam), anti‐GAPDH antibody (264 140, Abmart), anti‐BAX antibody (AB026, Beyotime), anti‐BCL‐XL antibody (AB126, Beyotime), anti‐BCL‐2 antibody (AB112, Beyotime), Dy Light 594 Goat‐anti Rabbit (A23420, Abbkine), Dy Light 488 Goat‐anti mouse (A23210, Abbkine).

Protein expression in total lung tissues, HPMECs, and PASMCs were determined by Western blotting. After transferation, the PVDF membranes (Millipore, United States) were blocked with 5% skim milk to prevent non-specific binding, then incubated with specific primary antibodies against HIF1-α (1:500, ab216842, Abcam, United States), NOX4 (1:1,000, 14347-1-AP, Proteintech, China), SOD2 (1:1,000, 24127-1-AP, Proteintech, China), SOD1(1:1,000, 10269-1-AP, Proteintech, China), Cleaved Caspase-3 (1:1,000, 9664, Cell Signaling Technology, United States), Bcl-xL (1:1,000, AB126, Beyotime, China), β-actin (1:2,500, 60008-1-Ig, Proteintech, China). After washing with TBST buffer, the membranes were incubated with appropriate secondary antibody conjugated with horseradish peroxidase, and signals were detected using by enhanced chemiluminescent kit (Amersham Biosciences, United Kingdom). The relative OD of Western blotting was calculated with the Image lab software (Bio-Rad Laboratories, Hercules, CA, United States).