Mafia

Manufactured by Jackson ImmunoResearch

Sourced in Montenegro

MAFIA is a protein detection and quantification platform developed by Jackson ImmunoResearch. It utilizes a magnetic-bead-based immunoassay technology to identify and measure target proteins in complex biological samples. The core function of MAFIA is to provide a sensitive and reliable method for protein analysis.

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MaFIA mice (3 citations) MAFIA (CSF1R-EGFP-NGFR/FKBP1A/TNFRSF6) transgenic mice (2 citations)

7 protocols using mafia

Genetically Modified Mice for Immunological Research

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C57BL/6, Scgb1a1-CreERT (stock# 016225), Irf4^fl/fl (stock# 009380) and MAFIA (stock #005070) mice were purchased from Jackson Laboratory (Bar Harbor, ME). Scgb1a1-CreERT;Csf1^fl/fland Csf1r-creERT;Irf4^fl/fl were generated as described previously^{11 (link)}. Mice were bred in a specific pathogen-free facility maintained by the University of Illinois at Chicago. All mouse experiments were approved by the Institutional Animal Care and Use Committee of University of Illinois at Chicago. Age & sex-matched 7 to 10-week-old mice were used for experiments.

Moon H.G., Kim S.J., Lee M.K., Kang H., Choi H.S., Harijith A., Ren J., Natarajan V., Christman J.W., Ackerman S.J, & Park G.Y. (2019). Colony stimulating factor 1 and its receptor are new potential therapeutic targets for allergic asthma. Allergy, 75(2), 357-369.

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Mammary Epithelial Cell Transplantation

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Animal procedures were conducted in compliance with Institutional Animal Care and Use Committee (IACUC) of Princeton University, University of Pennsylvania and Memorial Sloan Kettering Cancer Center. Dll1 floxed mice (33 (link)), Dll1-GFP-IRES-Cre-ERT2 mice (21 (link)) and CD11c-Cre; Rbpjk^cKO mice (41 (link)) mice have been described previously. The Dll1-mCherry transgenic mice, which were generated using a genomic BAC clone with mCherry cDNA inserted after the start codon of Dll1, will be described elsewhere (Gao & Aifantis, manuscript in preparation). tdTomato mice (B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J), Actin-GFP, Actin-dsRED mice and MaFIA mice were obtained from Jackson Laboratory. For all animal experiments, control littermate animals were utilized. For cleared fat pad injection experiment, C57/B6, athymic nude and NSG mice at 3 weeks old were anaesthetized and a small incision was made to reveal the mammary gland. Mammary epithelial cells (MECs) as specified in each experiment were injected into cleared inguinal (#4) mammary fat pads according to the standard procedures (48 (link)).

Chakrabarti R., Celià-Terrassa T., Kumar S., Hang X., Wei Y., Choudhury A., Hwang J., Peng J., Nixon B., Grady J.J., DeCoste C., Gao J., Van Es J., Li M.O., Aifantis I., Clevers H, & Kang Y. (2018). Dll1-mediated macrophageal niche for mammary gland stem cells. Science (New York, N.Y.), 360(6396), eaan4153.

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Characterization of Myeloid Cells in Mice

Cited 3 times

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Adult mice (4–12 weeks old) were used in all experiments. WT C57BL/6 mice, MAFIA (CSF1R-EGFP-NGFR/FKBP1A/TNFRSF6) transgenic mice^{22 (link)} (Stock #005070), CCL2 knockout mice^{53 (link)} (Stock #004434), and CCR2-RFP^+/+ knock-in mice^{54 (link)} (Stock #017586) were obtained from the Jackson Laboratory. We also studied CX3CR1^CreER-EYFP mice^{18 (link)} originally generated by Wen-Biao Gan at New York University. We crossed homozygous MAFIA mice with homozygous CCR2-RFP^+/+ mice to generate CSF1R-GFP^+/−CCR2-RFP^+/− mice. All animal experiments were approved by the Institutional Animal Care and Use Committee at University California San Francisco and were conducted in accordance with the NIH Guide for the Care and Use of Laboratory animals.

Yu X., Liu H., Hamel K.A., Morvan M.G., Yu S., Leff J., Guan Z., Braz J.M, & Basbaum A.I. (2020). Dorsal root ganglion macrophages contribute to both the initiation and persistence of neuropathic pain. Nature Communications, 11, 264.

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Knockout Mouse Strains for Immunology

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C57BL/6 mice were purchased from Charles River Laboratories. CCR2^−/− (catalog no. 004999), MaFIA (catalog no. 005070), Batf3^-/- (catalog no. 013755), CX3CR1^gfp/gfp (catalog no. 005582), LysM-cre (catalog no. 004781), CD11c-cre (catalog no. 008068) and iDTR (catalog no. 007900) mice were purchased from Jackson Laboratory and subsequently bred at the Hershey Medical Center. LysMcre:iDTR and CD11ccre:iDTR mice were heterozygotes derived from crossing purebred LysM-cre or heterozygous CD11ccre with purebred iDTR mice. All knockout mouse strains were on the C57BL/6 background after a minimum of 12 backcrosses.

Davies M.L., Parekh N.J., Kaminsky L.W., Soni C., Reider I.E., Krouse T.E., Fischer M.A., van Rooijen N., Rahman Z.S, & Norbury C.C. (2017). A systemic macrophage response is required to contain a peripheral poxvirus infection. PLoS Pathogens, 13(6), e1006435.

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Diet-Induced Obesity and T2DM in Mice

Cited 1 time

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Male C57Bl/6J mice (Jackson Laboratories, Bar Harbor, ME) or MaFIA (Macrophage associated Fas-Induced Apoptosis, #5070Jackson Laboratories, Bar Harbor, ME) were started on either a low fat diet (LFD; 10% Kcal, Research Diets #12450D) or high fat diet (HFD; 60% Kcal, Research Diets #D12492) beginning at 4-weeks of age, and were maintained on their respective diets for the duration of the experiment. Male C57Bl/6J mice were used as this strain and sex are very susceptible to diet-induced obesity and T2DM [8 (link), 9 (link)]. MaFIA mice express Green Fluorescent Protein (GFP) under the control of the Csf1r-promoter resulting in GFP expression in macrophages and dendritic cells. Mice were maintained in pathogen free housing on 12hr/12hr light/ dark cycle with ad libitum access to food and water (except during fasting when food was removed).

Ackerman J.E., Geary M.B., Orner C.A., Bawany F, & Loiselle A.E. (2017). Obesity/Type II diabetes alters macrophage polarization resulting in a fibrotic tendon healing response. PLoS ONE, 12(7), e0181127.

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Genetic Manipulation of Murine Macrophages

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IL-1R1 deficient mice and macrophage Fas-Induced Apoptosis (Mafia) transgenic mice were obtained from Jackson Laboratory (Bar Harbor, Maine) and bred in a biosafety level 1 (BL-1) facility at Harvard Medical School (HMS). Mafia mice were crossed with IL-1R1−/− mice to produce Mafia/IL-1R1−/− mice. C57BL/6J mice were purchased from Jackson Laboratory and used as WT controls. All mice were handled in accordance with guidelines set out by the Center for Animal Resources and Comparative Medicine at HMS.

Tian T., Jin M.Q., Dubin K., King S.L., Hoetzenecker W., Murphy G.F., Chen C.A., Kupper T.S, & Fuhlbrigge R.C. (2017). IL-1 receptor type 1 deficient mice demonstrate an impaired host immune response against cutaneous vaccinia virus infection. Journal of immunology (Baltimore, Md. : 1950), 198(11), 4341-4351.

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Mice Handling and Housing for Experiments

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All experiments involving mice and the procedures used therein were approved by the University of Iowa Institutional Animal Care and Use Committee and were carried out in strict accordance with the NIH Guide for the Care and Use of Laboratory Animals. Every effort was made to minimize the number of mice used and their suffering. Mice (6–10 weeks of age) were housed with food and water ad libitum under a 12-hr light/dark cycle. C57BL/6J, TRPV1 KO (Jackson Labs #003770; C57BL/6J), TRPA1 KO (Jackson Labs #006401; mixed B6;129) and MAFIA (Jackson Labs #005070; C57BL/6J) mice were obtained from The Jackson Laboratory (Farmington, CT).

Warwick C.A., Shutov L.P., Shepherd A.J., Mohapatra D.P, & Usachev Y.M. (2019). Mechanisms underlying mechanical sensitization induced by complement C5a: the roles of macrophages, TRPV1 and CGRP receptors. Pain, 160(3), 702-711.

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