Cellsens standard imaging software package

Deparaffinization and rehydration of 5 μm FFPE meningioma xenograft sections and hematoxylin and eosin staining were performed using standard procedures. Immunostaining was also performed on 5 μm FFPE meningioma xenograft sections using an automated Ventana Discovery Ultra staining system. Immunohistochemistry was performed using rabbit monoclonal Ki-67 (Ventana, clone 30-9, Cat# 790-4286, 1:6) with incubation for 16 min. Histologic and immunohistochemical features were evaluated using light microscopy on an BX43 microscope with standard objectives (Olympus). Images were obtained and analyzed using the Olympus cellSens Standard Imaging Software package.

For iPSC-derived NES^MYCN Hh-associated medulloblastomas, deparaffinization and rehydration of 5μm formalin-fixed, paraffin-embedded (FFPE) tissue sections and H&E staining were performed using standard procedures. All histological experiments were imaged on a BX43 light microscope (Olympus) and analyzed using the Olympus cellSens Standard Imaging Software package.