Anti cyclin d1 60186 1 ig

Manufactured by Proteintech

Sourced in United States

Anti-Cyclin-D1 (60186-1-Ig) is a primary antibody used for the detection of Cyclin-D1 protein expression in various applications, such as Western blotting, immunohistochemistry, and immunofluorescence.

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Lab products found in correlation

Deferoxamine mesylate, by Selleck Chemicals (1 mentions) 3 ma hy 19312, by MedChemExpress (1 mentions) Z vad fmk s7023, by Selleck Chemicals (1 mentions) Anti bcl 2, by Cell Signaling Technology (1 mentions) Anti e cadherin, by Cell Signaling Technology (1 mentions) Anti phospho iκbα, by Cell Signaling Technology (1 mentions) Dimethyl sulfoxide (dmso), by Wuhan Servicebio Technology (1 mentions) Anti bad, by Cell Signaling Technology (1 mentions) Erastin hy 15763, by MedChemExpress (1 mentions) Anti p65 sc 8008, by Santa Cruz Biotechnology (1 mentions) Anti zeb 1 21544 1 ap, by Proteintech (1 mentions) Anti nrf2 m200 3, by Medical & Biological Laboratories (1 mentions) Anti gpx4 67763 1 ig, by Proteintech (1 mentions) Anti gapdh 60004 1 ig, by Proteintech (1 mentions) Bm0627, by Boster Bio (1 mentions) Anti n cadherin 22018 1 ap, by Proteintech (1 mentions) Bm0135, by Boster Bio (1 mentions) Ab109268, by Abcam (1 mentions) Anti mmp2 10 373 2 ap, by Proteintech (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions)

4 protocols using anti cyclin d1 60186 1 ig

Ferroptosis Regulation by Molecular Inhibitors

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Myrislignan (S3261), Z-VAD-FMK (S7023), necrosulfonamide (S8251), ferrostatin-1 (S7243), deferoxamine mesylate (S5742), and RSL3 (S8155) were purchased from Selleck (USA). 3-MA (HY-19312) and erastin (HY-15763) were purchased from MedChemExpress (USA). Dimethyl sulfoxide (DMSO) was obtained from Servicebio (G5051, China). The following antibodies were used: anti-GAPDH (60004-1-Ig, Proteintech), anti-E-cadherin (14472, Cell Signaling Technology), anti-Snail-1 (13099-1-AP, Proteintech), anti-Slug (GTX128796, GeneTex), anti-p65 (sc-8008, Santa Cruz), anti-p-p65 (sc-136548, Santa Cruz), anti-phospho-IκB-α (2859, Cell Signaling Technology), anti-IκB-α (CSB-RA015761A0HU, Cusabio Technology), anti-SLC7A11 (NB300-318, Novus), anti-β-catenin (phospho Y142, ab27798, Abcam), anti-β-catenin (ab32572, Abcam), anti-Cyclin-D1 (60186-1-Ig, Proteintech), anti-Bad (9292, Cell Signaling Technology), anti-Bcl2 (15017, Cell Signaling Technology), anti-Vimentin (GB111308, Servicebio), anti-Twist (25465-1-AP, Proteintech), anti-ZEB1 (21544-1-AP, Proteintech), anti-Nrf2 (M200-3, Medical and Biological Laboratories), anti-TFR1 (ab214039, Abcam), and anti-GPX4 (67763-1-Ig, Proteintech).

Zhou Y., Qian W., Li X, & Wei W. (2023). NF-κB Inhibitor Myrislignan Induces Ferroptosis of Glioblastoma Cells via Regulating Epithelial-Mesenchymal Transformation in a Slug-Dependent Manner. Oxidative Medicine and Cellular Longevity, 2023, 7098313.

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Protein Expression Analysis in Renal Cancer Cells

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Proteins of 786-O and OS-RC-2 cells were extracted using RIPA buffer, and they were separated on 10% SDS/PAGE gels. Next, the proteins were transferred onto polyvinylidene fluoride (PVDF) membranes. After using 5% nonfat milk to block membranes for 1 h at room temperature, they were incubated by proper concentration of primary antibodies overnight at 4°C. The following primary antibodies were used in our research: anti–β-actin (BM0627, Boster), anti-PCNA (10205-2-AP, Proteintech), anti–Cyclin D1 (60186-1-Ig, Proteintech), anti–N-Cadherin (22018-1-AP, Proteintech), anti-Vimentin (BM0135, Boster), anti-ZEB1 (BA2871–2, Boster), and anti-Snail (13099-1-AP, Proteintech). Anti-mouse (31,430, Thermo Scientific) or anti-rabbit (31,460, Thermo Scientific) IgG secondary antibody were applied at the concentration of 1:10,000.

Sun G., Ge Y., Zhang Y., Yan L., Wu X., Ouyang W., Wang Z., Ding B., Zhang Y., Long G., Liu M., Shi R., Zhou H., Chen Z, & Ye Z. (2021). Transcription Factors BARX1 and DLX4 Contribute to Progression of Clear Cell Renal Cell Carcinoma via Promoting Proliferation and Epithelial–Mesenchymal Transition. Frontiers in Molecular Biosciences, 8, 626328.

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Phosphorylation Pathway Modulation Protocol

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GNE-477 [S6516, purity (98.43%)] (Figure 1A) and the AKT phosphorylation activator SC79 (S7863, purity (>97%)) were both purchased from Selleckchem (Houston, TX, United States), and GNE-477 was dissolved and preserved in dimethyl sulfoxide (DMSO) obtained from Servicebio (G5051, Wuhan, China). The following antibodies were used: anti-AKT (product no.4691, Cell Signaling Technology (CST), United States), anti-phospho-AKT (product no. 4060; CST, United States), anti-CyclinD1 (60,186-1- Ig, Proteintech, Wuhan, China), anti-MMP2 (10,373-2- AP, Proteintech, Wuhan, China), anti-Bax (50599-2-Ig, Proteintech, Wuhan, China), anti-Bad (product no. 9239,CST,United States), anti-Bcl-2 (127891-AP,Proteintech, Wuhan, China), anti-mTOR (20657-1-AP, Proteintech, Wuhan, China), anti-phospho-mTOR (ab109268, Abcam, United Kingdom), and anti GAPDH (60004-1-Ig, Proteintech).

Wang Y., Shen H., Sun Q., Zhao L., Liu H., Ye L., Xu Y., Cai J., Li Y., Gao L., Tan Y., Liu B, & Chen Q. (2021). The New PI3K/mTOR Inhibitor GNE-477 Inhibits the Malignant Behavior of Human Glioblastoma Cells. Frontiers in Pharmacology, 12, 659511.

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Profiling β-catenin Signaling Pathway

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Immunoprecipitation and Western blotting were performed as described previously.^{35 (link)} GAPDH protein levels were used as loading controls. We used the following primary antibodies: anti-Non-phospho (Active) β-catenin (Ser33/37/Thr41) (8814, Cell Signaling Technology), anti-phospho-β-catenin (Ser33/37/Thr41) (9561, Cell Signaling Technology), anti-β-catenin (51067-2-AP, Proteintech), anti-β-TrCP (4394, Cell Signaling Technology), anti-FAM83A (A15201, ABclonal), anti-c-myc (10828-1-AP, Proteintech), anti-CyclinD1 (60186-1-Ig, Proteintech), anti-AXIN2 (20540-1-AP, Proteintech), anti-mouse HA (M180-3, EMD Millipore), anti-Rabbit HA (51064-2-AP, Proteintech), anti-mouse DYKDDDDK (M185, MBL), anti-Rabbit DYKDDDDK (80010-1-RR, Proteintech), anti-GAPDH (60004-1-Ig, Proteintech), anti-GFP (598, EMD Millipore), anti-phosphotyrosine (P4110, Sigma), anti-phosphoserine (05-1000x, EMD Millipore), anti-phospho-threonine (9386 S, Cell Signaling Technology), anti-GSK3β (22104-1-AP, Proteintech), anti-AXIN1 (16541-1-AP, Proteintech), anti-TCF4 (22337-1-AP, Proteintech), anti-BLK (10510-1-AP, Proteintech), anti-HDAC1 (10197-1-AP, Proteintech), anti-HDAC2 (12922-2-AP, Proteintech), anti- Acetyl-Histone H3 (8173, Cell Signaling Technology).

Zhou C., Zhu X., Liu N., Dong X., Zhang X., Huang H., Tang Y., Liu S., Hu M., Wang M., Deng X., Li S., Zhang R., Huang Y., Lyu H., Xiao S., Luo S., Ali D.W., Michalak M., Chen X.Z., Wang Z, & Tang J. (2023). B-lymphoid tyrosine kinase-mediated FAM83A phosphorylation elevates pancreatic tumorigenesis through interacting with β-catenin. Signal Transduction and Targeted Therapy, 8, 66.

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