Ampicillin sodium salt, kanamycin sulphate, chloramphenicol, carbenicillin disodium salt, oxacillin sodium salt, and gentamicin solution were purchased from Sigma-Aldrich(Rehovot, Israel). Polymixin B was purchased from Fluka BioChemika (Seelze, Germany). and tetracycline hydrochloride was purchased from Sigma-Aldrich (Rehovot, Israel).
Oxacillin sodium salt
Manufactured by Merck Group
Sourced in United States
Oxacillin sodium salt is a semi-synthetic penicillin antibiotic used in the laboratory setting. It functions as an inhibitor of bacterial cell wall synthesis, disrupting the formation of the peptidoglycan layer.
Automatically generated - may contain errors
Lab products found in correlation
Chloramphenicol, by Merck Group (2 mentions)
Vancomycin hydrochloride, by Merck Group (2 mentions)
Dopamine hydrochloride, by Merck Group (2 mentions)
Ampicillin sodium salt, by Merck Group (1 mentions)
Kanamycin sulphate, by Merck Group (1 mentions)
Carbenicillin disodium salt, by Merck Group (1 mentions)
Tetracycline hydrochloride, by Merck Group (1 mentions)
Gentamicin solution, by Merck Group (1 mentions)
Ceftazidime hydrate, by Merck Group (1 mentions)
Cefotaxime, by Merck Group (1 mentions)
Piperacillin, by Merck Group (1 mentions)
Cefotaxime sodium salt, by Merck Group (1 mentions)
Difco lb broth, by BD (1 mentions)
Sob media, by Merck Group (1 mentions)
Potassium clavulanate, by Merck Group (1 mentions)
Oxacillin, by Merck Group (1 mentions)
Allantoin, by Merck Group (1 mentions)
Ferric chloride hexahydrate, by Merck Group (1 mentions)
Glutaraldehyde solution, by Merck Group (1 mentions)
Ammonia solution, by Merck Group (1 mentions)
7 protocols using oxacillin sodium salt
1
Antibiotic Compounds Acquisition
2
Antibiotic Susceptibility Testing Protocol
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We used Difco LB broth (BD) for all experimental steps involving growth and selection. We used SOB media (Sigma) for preparing competent cells and SOC media (SOB media with 20 mM glucose) for recovery of electroporated cells. For antibiotic selection, we used chloramphenicol (Sigma) at 25 and 34 μg ml−1, cefotaxime sodium salt (Sigma) at concentrations 0.0078–2,048 μg ml−1, cefoxitin sodium salt (Apollo Scientific) at concentrations 0.5–32 μg ml−1, oxacillin sodium salt (Sigma) at concentrations 32–2,048 μg ml−1, piperacillin (Sigma) at concentrations 64–4,096 μg ml−1, potassium clavulanate (Sigma) at concentrations 0.1 μg ml−1 (with cefotaxime) and 0.5 μg ml−1 (with oxacillin) and ceftazidime hydrate (Sigma) at 0.25–256 μg ml−1. We used saline (9 g l−1 NaCl) to prepare serial dilutions for library size estimations.
3
Synthesis of Multifunctional Nanoparticles
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Iron (II, III) oxide, dopamine hydrochloride, allantoin, vancomycin hydrochloride, and oxacillin sodium salt were procured from Sigma-Aldrich (St. Louis, MO, USA). Phosphate-buffered saline (PBS, pH 7.4) was obtained from Gibco (Grand Island, NY, USA).
4
Synthesis of Functionalized Silica Nanoparticles
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Ferric chloride hexahydrate, sodium acetate, ethylene glycol, 25% ammonia solution, 99% tetraethoxysilane (TEOS), (3-aminopropyl)triethoxysilane (APTES), 25% glutaraldehyde solution, sodium tetraborate, dopamine hydrochloride, vancomycin hydrochloride, oxacillin sodium salt and 99% osmium tetroxide (OsO4) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate-buffered saline (PBS, pH 7.4) was purchased from Gibco (Grand Island, NY, USA).
5
Antimicrobial and Cytotoxic Evaluation of Natural Compounds
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Chloroform was obtained from Thai Oil Co. Ltd. (Thailand). Sodium alginate and pectin were purchased from Sigma-Aldrich Company. Dimethyl sulfoxide (DMSO) was purchased from Sigma-Aldrich (Germany). Mueller Hinton Agar (MHA) and trypticase soy agar (TSA) were obtained from Difco, Bacto Dickinson, Spark (USA). Oxacillin paper discs were purchased from Oxoid Limited (UK). Oxacillin sodium salt was purchased from Fluka, Sigma-Aldrich (Denmark). Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), 0.25% trypsin EDTA, trypan blue, phosphate-buffered saline (PBS), penicillin, streptomycin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), glycerin, Span 60, and cholesterol were purchased from Gibco® (Life Technologies, Paisley, Scotland). RCI Labscan Ltd. (Bangkok, Thailand) provided all of the solvents.
6
Antibiotic Stock Preparation and Storage
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The following antibiotics cefepime hydrochloride (#PHR1763-1G:LRAB8503, sigma), ceftriaxone disodium salt hemi-heptahydrate (#C5793-1G: 027M4799, sigma), ceftazidime (#CDS020667-50MG:B02487097), oxacillin sodium salt (Cat#28221-5G:097M4853V), imipenem (#PHR1796-200MG:LRAB7177, sigma), doripenem hydrate (#SML1220-50MG:025M4717V, sigma), meropenem trihydrate (#USP-1392454: 50K434), ertapenem (#CDS022172-25MG:B02467020) were procured in dry powder and stored in 4oC under desiccated conditions. Master stocks of 1024 μg/mL were made in sterile distilled water and stored in -80oC for future use. Every time fresh stock of antibiotics was used for experiments. All the other chemicals are of ACS analytical grade.
7
MRSA Antibiotic Susceptibility Testing
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This study was conducted with the 2019/1089 numbered permission of the ethics committee. It was carried out in accordance with the rules of scientific research and publication ethics. Ten MRSA clinical strains used in the study were obtained from the Microbiology Department of the Central Laboratory of Akdeniz University Hospital. Stock solutions of S. aureus strains which were isolated from clinical samples were cultured on Blood Agar (Becton Dickinson, USA). Following incubation at 35±2°C for 18-24 hours, the colonies were identified by MALDITOF MS (Bruker Biotyper Daltonik, Germany) method. Antibiotic susceptibilities of colonies which identified as S. aureus were analyzed by BD Phoenix automated system (Becton Dickinson, USA). Ten strains identified as MRSA were included in the study. S. aureus ATCC 43300 (methicillin-resistant strain) and S. aureus ATCC 29213 (methicillin-sensitive strain) were used as quality control strains. 512 µg/ml oxacillin sodium salt (28221, Sigma-Aldrich) stock solution was prepared in distilled water. Carvacrol (W224511, Sigma-Aldrich) was dissolved in pure ethanol to prepare 10 mg/ml stock solution.
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