Blood was collected from the IVC, and the livers were recovered. Serum alanine transaminase (ALT) was measured using analytical kits from Pointe Scientific (Uncoln Park, MI) [8 (link)]. Histology was performed using hematoxylin-eosin (H&E) staining. Necrotic areas were quantified in a blinded manner by image analysis of 10 randomly selected fields per liver in slides stained with H&E using a IPlab 3.7v software (BD Biosciences, Rockville, MD) [9 (link)]. Apoptosis was assessed by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) using an In Situ Cell Death Detection Kit [10 (link)]. RT-PCR was used to determine the expression of transforming growth factor-β (TGF-β) mRNA.
Iplab 3.7v software
Manufactured by BD
IPlab 3.7v software is a laboratory analysis application designed for data processing and visualization. It provides core functionality for managing and analyzing experimental data from various sources.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using iplab 3.7v software
1
Liver Injury Histological and Molecular Assessment
2
Liver Histological Analysis after Transplantation
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Liver tissue was collected at 6 hr after transplan-tation or sham operation. Hematoxylin-eosin (H&E) staining was used to observe the liver histology. Necrotic areas were quantified by image analysis of 10 randomly selected fields in H&E-stained slides using IPlab 3.7v software (BD Biosciences, Rockville, MD) (24 (link)). Apoptosis was evaluated by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) with an in situ cell death detection kit as described elsewhere (25 (link)).
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