Female BALB/c-nu/nu mice were purchased from Charles River Laboratories Japan, Inc. (Kanagawa, Japan). Jcl:ICR mice were purchased from Clea Japan, Inc. (Tokyo, Japan). All animal procedures were performed according to protocols approved by the Japanese Foundation for Cancer Research Animal Care and Use Committee.
Jcl icr mice
Manufactured by CLEA Japan
Sourced in Japan
The Jcl:ICR mice are a commonly used mouse strain in biomedical research. They are outbred mice, meaning they are genetically diverse. The Jcl:ICR mice are known for their high reproductive performance and are widely used as general-purpose experimental animals.
Automatically generated - may contain errors
Lab products found in correlation
Ni nta superflow, by Qiagen (2 mentions)
Calf serum, by Thermo Fisher Scientific (2 mentions)
Minimal essential medium without phenol red mem, by Thermo Fisher Scientific (2 mentions)
Basal medium eagle bme, by Merck Group (2 mentions)
Flexiperm, by Sarstedt (2 mentions)
Balb c nu nu female mice, by Charles River Laboratories (1 mentions)
Procise 491ht, by Thermo Fisher Scientific (1 mentions)
Escherichia coli bl21 star de3, by Thermo Fisher Scientific (1 mentions)
Clea rodent diet ce 2, by CLEA Japan (1 mentions)
Jcl icr, by CLEA Japan (1 mentions)
Bl21 star de3, by Thermo Fisher Scientific (1 mentions)
Midazolam, by Fujifilm (1 mentions)
Medetomidine hydrochloride, by Fujifilm (1 mentions)
Butorphanol tartrate, by Fujifilm (1 mentions)
Luria bertani broth, by BD (1 mentions)
G8540, by Merck Group (1 mentions)
Cytosine β d arabinofuranoside, by Merck Group (1 mentions)
13 protocols using jcl icr mice
1
Mouse Xenograft Protocol for Cancer Research
2
Murine Neutropenic Lung Infection Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Briefly, for the neutropenic murine lung infection model, male Jcl:ICR mice (weighing 17 to 20 g [CLEA Japan, Inc., Tokyo, Japan]) were rendered neutropenic by two doses of intraperitoneal cyclophosphamide prior to the experiment (150 mg/kg on day −4 and 100 mg/kg on day −1). Anesthetized mice were inoculated with 3 to 5 × 106 CFU of S. maltophilia . Antibiotics (in a 0.9% saline vehicle) were administered subcutaneously at 2, 5, and 8 h postinfection (5 animals/dosing group). Control mice were not treated with antibiotics and received either no intervention (untreated) or vehicle (vehicle-treated). Following the initial infection, mice were euthanized at 2 h (untreated controls) or 24 h (antibiotic-treated animals or vehicle-treated controls), lungs were excised, and the numbers of viable cells in lung tissue were counted. Dunnett’s multiple-comparison test was used to test differences between active treatment and untreated baseline control groups in the number of viable cells following treatment. Welch’s t test was used to compare differences between comparator antibiotics cefiderocol at the same doses. The P value for significance in both tests was <0.05.
3
Murine Model of Experimental Infection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Five-week-old, specific-pathogen-free, male Jcl:ICR mice (weight, 17 to 20 g) were obtained from CLEA Japan, Inc. (Tokyo, Japan). Three to five mice per group were used in all experimental infection models. All studies with animals were approved by the Institutional Animal Care and Use Committee of Shionogi & Co., Ltd.
4
Polyclonal Antibody Generation against VtgA and VtgB
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Polyclonal antibodies against VtgA and B were generated in this study. The antigen sequences are aa 901–1,018 (VtgA, GenBank: ACI30217.1) and aa 902–993 (VtgB, GenBank: ACI30218.1). Inclusion bodies of the antigen peptides were harvested from the transformant Escherichia coli BL21 Star (DE3) (Thermo Fisher Scientific, Waltham, MA) (39 (link)). For determination of N-terminal sequences of the antigen protein, the inclusion bodies loaded onto Ni-NTA Superflow (Qiagen, Valencia, CA) were subjected to a protein sequencer (Procise 491HT; Applied Biosystems, Foster City, CA). Milligrams (0.2 mg) of each inclusion body diluted in urea/PBS were injected into Jcl:ICR mice (CLEA Japan, Inc., Meguro, Japan) 4 times every 2 wk. Serum samples were harvested from the antigen-injected mice. The sera, diluted to 50% in glycerol with 0.1% sodium azide, were used for immunohistochemistry. Information for the commercial antibodies used is listed in SI Appendix, Table S1 .
5
Timed-pregnant Jcl:ICR Mice Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Timed-pregnant Jcl:ICR mice (Catalog ID: Jcl:ICR, CLEA Japan, Inc., Tokyo, Japan) were purchased at gestational day 15 from the Kyudo Company (Tosu, Japan). Fifteen to seventeen-week-old pregnant Jcl:ICR mice were used. The pregnant mice were housed in plastic cages in an environment with a room temperature of 23 ± 2°C, a humidity of 60 ± 2%, and a 12 h light-dark cycle (lights on at 7:00 AM, lights off at 7:00 PM). Food (CLEA Rodent Diet, CE-2, CLEA Japan, Inc., Tokyo, Japan) and water were provided ad libitum. The body weights of pregnant mice were not recorded.
Experimental animals were handled in accordance with the animal ethics regulations of the Fukuoka University Animal Care and Use Committee (Approval Nos. 2112094 and 2311081).
Experimental animals were handled in accordance with the animal ethics regulations of the Fukuoka University Animal Care and Use Committee (Approval Nos. 2112094 and 2311081).
6
Generation and Maintenance of Pkr2-/- Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The generation of Pkr2−/− was originally described in our previous report [18 (link)]. The background of the mice was originally C57BL/6 J, but, with the low survival ratio of neonates, the genetic background was changed to the ICR strain. The colony used in the present study was established by 11 backcrosses with Jcl:ICR mice (CLEA Japan, Tokyo). The resulting strain was subsequently maintained by interbreeding for at least 10 generations. Genotypes were determined using an established PCR method. Wild type (WT) and mutant adult mice (n = 3) at 12–15 weeks old were used for comparison. This study was performed in compliance with the Rules and Regulations of the Animal Care and Use Committee of Kindai University School of Medicine, and adhered to the Guide for the Care and Use of Laboratory Animals, Kindai University School of Medicine.
7
Superovulation in Aged and Young C57BL/6J Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C57BL/6J Jcl and Jcl:ICR mice purchased from CLEA Japan, Inc. (Tokyo, Japan) and bred at
the Kasumi Animal Center of the Natural Science Center for Basic Research and Development
at Hiroshima University. To examine superovulation, 16- and 52-week-old C57BL/6J females
were used as young and aged mice, respectively. To prepare pseudopregnant females for the
embryo transfer procedure, mature females and vasectomized males of the ICR strain were
used. All experiments were carried out with the permission of the Committee of Animal
Experimentation at Hiroshima University (approval number: A14-7).
the Kasumi Animal Center of the Natural Science Center for Basic Research and Development
at Hiroshima University. To examine superovulation, 16- and 52-week-old C57BL/6J females
were used as young and aged mice, respectively. To prepare pseudopregnant females for the
embryo transfer procedure, mature females and vasectomized males of the ICR strain were
used. All experiments were carried out with the permission of the Committee of Animal
Experimentation at Hiroshima University (approval number: A14-7).
8
Polyclonal Antibody Production for Proteomic Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse polyclonal antibodies against ZPb, c5, AX1, and ChgL were generated. The antigen sequences are listed in Table S7 . Inclusion bodies of the antigen proteins were harvested from the transformant Escherichia coli BL21 Star (DE3) (Thermo Fisher Scientific). Inclusion bodies dissolved in 2M urea/PBS were loaded onto a Ni-NTA Superflow (Qiagen) and purified. Approximately 0.2 mg of each eluted inclusion body solution was injected into Jcl:ICR mice (CLEA Japan, Inc) four times every 2 weeks. Serum samples were harvested from the antigen-injected mice. The serum, diluted to 50% in glycerol with 0.1% sodium azide, was used for immunohistochemistry.
9
Immunosuppressed Mouse Model of EC-14 Infection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All experiments were conducted in accordance with the ethical standards of our university (Animal Care Committee of Kanazawa University, AP-183983) and with international standards for animal welfare and institutional guidelines. EC-14 was cultured in Luria–Bertani broth (Becton, Dickinson and Co., Franklin Lakes, NJ, USA) for 12 h and then seeded into new broth and incubated at 37 °C for 12–14 h with shaking. Subsequently, EC-14 was collected by centrifugation at 8000× g for 5 min at 4 °C and suspended for inoculation into mice. Male Jcl:ICR mice (n = 4) (4 weeks old; CLEA Japan, Tokyo, Japan) were purchased 7 days prior to the experiments and subjected to immunosuppression treatment with 150 mg/kg and 100 mg/kg of endoxan (Shionogi) at 4 days and 1 day prior to infection, respectively.
EC-14 (approximately 5 × 106 CFU/100 µL) was injected into the muscle of the hind leg of mice under anesthesia. Mice were anesthetized using a mixture of butorphanol tartrate, midazolam, and medetomidine hydrochloride (Fujifilm Wako Pure Chemical Corp., Osaka, Japan). Mice were euthanized at 2, 6, 8 and 24 h after infection. Subsequently, the infected muscle was collected and homogenized in PBS. The number of CFU in the homogenized samples was determined by dilution in PBS and plating on agar medium.
EC-14 (approximately 5 × 106 CFU/100 µL) was injected into the muscle of the hind leg of mice under anesthesia. Mice were anesthetized using a mixture of butorphanol tartrate, midazolam, and medetomidine hydrochloride (Fujifilm Wako Pure Chemical Corp., Osaka, Japan). Mice were euthanized at 2, 6, 8 and 24 h after infection. Subsequently, the infected muscle was collected and homogenized in PBS. The number of CFU in the homogenized samples was determined by dilution in PBS and plating on agar medium.
10
Culturing Cerebellar Granule Neurons
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CGNs were cultured at low density as described previously (Kubota et al., 2013) . Mice were treated according to the institutional ethical guidelines of the University of Fukui (number: R02901). Postnatal day (P) 5-6 mice were obtained by crossing Jcl:ICR mice (CLEA Japan, Tokyo, Japan) that had been maintained at 18-28°C under a controlled light cycle (10 h light/14 h dark).
Mice were decapitated, and isolated cerebella were digested with trypsin (TRL, Worthington, Lakewood, NJ). CGNs were suspended in Minimal Essential Medium (11090-081, Thermo Fisher Scientific, Waltham, MA) supplemented with 10% calf serum (SH30072.03, Thermo Fisher Scientific), penicillin (100 units/ml, P7794, Sigma-Aldrich, St. Louis, MO), streptomycin (0.1 mg/ml, P9137, Sigma-Aldrich), glutamine (2 mM, G8540, Sigma-Aldrich), and KCl (25 mM).
CGNs were plated onto round cover glass (13 mm) that has been attached with paraffin dots as spacers and pre-coated with 15 µg/ml poly-L-ornithine at 2-4 × 10 4 cells/well of a 24 well plate.
After several hours of incubation (37°C, 5% CO 2 ), CGNs on the cover slip were placed in another well containing a high density of CGNs (2.5 × 10 5 cells/well) so that cells faced each other across the spacers to support survival (Kubota et al., 2013) . Cytosine β-D-arabinofuranoside (10 µM, 034-11,954, Sigma-Aldrich) was added to the culture at 1 day in vitro.
Mice were decapitated, and isolated cerebella were digested with trypsin (TRL, Worthington, Lakewood, NJ). CGNs were suspended in Minimal Essential Medium (11090-081, Thermo Fisher Scientific, Waltham, MA) supplemented with 10% calf serum (SH30072.03, Thermo Fisher Scientific), penicillin (100 units/ml, P7794, Sigma-Aldrich, St. Louis, MO), streptomycin (0.1 mg/ml, P9137, Sigma-Aldrich), glutamine (2 mM, G8540, Sigma-Aldrich), and KCl (25 mM).
CGNs were plated onto round cover glass (13 mm) that has been attached with paraffin dots as spacers and pre-coated with 15 µg/ml poly-L-ornithine at 2-4 × 10 4 cells/well of a 24 well plate.
After several hours of incubation (37°C, 5% CO 2 ), CGNs on the cover slip were placed in another well containing a high density of CGNs (2.5 × 10 5 cells/well) so that cells faced each other across the spacers to support survival (Kubota et al., 2013) . Cytosine β-D-arabinofuranoside (10 µM, 034-11,954, Sigma-Aldrich) was added to the culture at 1 day in vitro.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!