WB analysis was carried out by the method previously described (58 (link), 60 (link), 63 ). Briefly, protein samples were extracted and applied to electrophoresis using the Novex NuPAGE SDS-PAGE Gel System (Thermo Fisher Scientific), followed by membrane transfer, antibody incubation, and signal development. The house-keeping protein, β-actin, was used as an internal control. The Odyssey Fc with Image Studio imaging software (LI-COR) was used to develop the blots and quantify the proteins of interest according to protocols previously described (58 (link), 60 (link), 63 ).
Odyssey fc with image studio imaging software
Manufactured by LI COR
The Odyssey Fc is a compact, all-in-one imaging system that combines a high-performance, cooled CCD camera with advanced image analysis software, Image Studio. The system is designed for quantitative fluorescence and chemiluminescence detection and imaging. It features a high-resolution optical system, a versatile sample handling platform, and intuitive software for data acquisition and analysis.
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2 protocols using odyssey fc with image studio imaging software
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Western Blot Analysis Methodology
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Western Blot Analysis Protocol
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WB analysis was carried out by the method previously described (Ward et al., 2017 (link); Zhang et al., 2010a (link), b (link)). Briefly, proteins were extracted and applied to electrophoresis using the Novex NuPAGE SDS-PAGE Gel System (Thermo Fisher Scientific), followed by membrane transfer, antibody incubation, and signal development. The house-keeping protein, β-actin or GAPDH, was used as an internal control. The VersaDoc imaging system with Quantity One software (Bio-Rad) and the Odyssey® Fc with Image Studio imaging software (LI-COR) were used to develop the blots and quantify the proteins of interest following protocols previously described (Ward et al., 2017 (link); Zhang et al., 2010a (link), b (link)). ImageJ was also used for the analyzing of blots.
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