Tissue culture plasticware

Tissue culture plastic ware was purchased from BD Bio-sciences (CA, USA). Dulbecco's Modified Eagles Medium (DMEM), penicillin and streptomycin were obtained from Gibco BRL (CA, USA). Fetal bovine serum (FBS) was purchased from SAF (USA). Bovine serum albumin (BSA) fatty acid free, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), Epidermal Growth Factor(EGF), Insulin, Hydrocortisone and the other reagents were obtained from Sigma-Aldrich (St. Louis, MO, USA). EPA was purchased from MERCK whereas DHA and AA were purchased from Cayman. α-Tubulin, p21^WAF1/CIP1 and Cux/CDP antibodies were obtained from Santa Cruz Biotechnology Inc.(CA, USA). SMAR1 antibody was purchased from Bethyl laboratories, Montgomery, USA. All other common reagents were procured from Qualigens Fine chemicals and Himedia (Mumbai, India).

Tissue culture plasticware was purchased from BD Biosciences (CA, USA). MDAMB231, MCF7, MCF10A and HEK 293 were obtained from National Centre for Cell Science (NCCS), Pune. The cells were grown in DMEM supplemented with 2 mM L-glutamine, 100 units/ml of penicillin/streptomycin, and 10% fetal bovine serum (FBS). They were incubated in a humidified 5% CO₂ incubator at 37°C.

Tissue culture plasticware was purchased from BD Biosciences, (San Jose, CA, USA). Dulbecco’s Modified Eagle’s Medium (DMEM) and Fetal Bovine Serum (FBS) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Penicillin/streptomycin and L-glutamine were obtained from Thermo Fisher Scientific, Gibco BRL (Waltham, MA, USA).