Myricetin

Manufactured by Solarbio

Sourced in China

Myricetin is a flavonoid compound found in various plant sources. It functions as an antioxidant by scavenging free radicals and inhibiting oxidative stress.

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Lab products found in correlation

Myricetin, by Merck Group (1 mentions) Pyaps127, by Cell Signaling Technology (1 mentions) Anti caspase 3, by Proteintech (1 mentions) Pcdna4 hismaxb yap1, by Addgene (1 mentions) Lats1, by Proteintech (1 mentions) Lats2, by Proteintech (1 mentions) Cyr61, by Proteintech (1 mentions) Survivin, by Proteintech (1 mentions) Mg132, by Merck Group (1 mentions) C myc, by Proteintech (1 mentions) Cisplatin, by Solarbio (1 mentions) Resazurin, by Solarbio (1 mentions) 3 ma s2767, by Selleck Chemicals (1 mentions) Catechin, by Solarbio (1 mentions) Epicatechin, by Solarbio (1 mentions) Cyanidin 3 o glucoside, by Solarbio (1 mentions) Trolox, by Solarbio (1 mentions) 2 4 6 tri 2 pyridyl 1 3 5 triazine tptz, by Solarbio (1 mentions) 1 1 diphenyl 2 picrylhydrazyl dpph, by Solarbio (1 mentions) 2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid abts, by Solarbio (1 mentions)

4 protocols using myricetin

Screening Assays for Myricetin Derivatives

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M10 (purity ≥ 98%) was provided by Dr. Li in Marine Biomedical Research Institute of Qingdao. M10 was fully dissolved in water. The design, synthesizing and analysing the properties of derivatives of Myricetin were published in the reference (Zhu et al., 2019 (link)). The following screening assays for M10 were carried out by Dr. Wang as shown in the reference (Wang et al., 2018 (link)). Myricetin (purity > 99%) was purchased from Sigma Chemical Co. (St. Louis, MO). Mesalazine was purchased from Anhui Dongsheng Pharmaceutical Co., Ltd. Myricetin and Mesalazine were prepared in 0.5% sodium carboxymethyl cellulose solvents (Solarbio, Beijing, China) before use.

Zhou X.L., Yang J., Qu X.J., Meng J., Miao R.R, & Cui S.X. (2020). M10, a Myricetin-3-O-b-D-Lactose Sodium Salt, Prevents Ulcerative Colitis Through Inhibiting Necroptosis in Mice. Frontiers in Pharmacology, 11, 557312.

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Comprehensive Molecular Analysis of YAP/LATS Signaling

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The biochemical reagents myricetin (#SM8390) and cisplatin (#D8810) were purchased from Solarbio (Beijing, China). MG132 (#M8699) was purchased from Sigma (St. Louis, MO, USA). The plasmids pcDNA4/HisMaxB-YAP1 (#18978), pCIneoMyc-LATS1 (#66851), and pCIneoMyc-LATS2 (#66852) were purchased from Addgene (Watertown, MA, USA). The following antibodies were used: anti-Caspase 3 (66470-1-Ig), YAP (13584-1-AP), c-Myc (#10828-1-AP), survivin (#10508-1-AP), CYR61 (#26689-1-AP), LATS1 (#17049-1-AP), LATS2 (#20276-1-AP), CTGF (#23936-1-AP) and Actin (#60008-1-Ig) (Proteintech Group, Chicago, IL, USA); p-YAP-S127 (#13008) (Cell Signaling Technology, Beverly, MA, USA); and p-LATS1/2-S909/872 (#AF8163) (Affinity Biosciences, Cincinnati, OH, USA).

Li M., Chen J., Yu X., Xu S., Li D., Zheng Q, & Yin Y. (2019). Myricetin Suppresses the Propagation of Hepatocellular Carcinoma via Down-Regulating Expression of YAP. Cells, 8(4), 358.

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Myricetin Modulates Autophagy and Apoptosis Pathways

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Myricetin, Hoechst Staining Kit (G3680) and resazurin were purchased from Solarbio Life Sciences (Beijing, China). 3-MA (S2767) was purchased from Selleck Chemicals (Houston, TX, USA). The primary antibodies included Bax (5023S), Bcl-2 (15071S), Microtubule-associated protein1 light chain 3 (LC-3) I/II (12741S), Beclin-1 (3495S), Phosphatidyl inositide 3-kinase (PI3K) (13666S), phospho-PI3K (4228S), phospho-Akt (4060S), Akt (2920S), Mammalian target of rapamycin (mTOR) (2983S), phospho-mTOR (5536S) were purchased from Cell Signaling (MA, USA). β-actin (60008–1) was purchased from ProteinTech Group (Wuhan, China). Horseradish peroxidase-conjugated secondary antibodies (ZB-2301, ZB-2305) were purchased from ZSGB-Bio (Beijing, China).

Zhu M.L., Zhang P.M., Jiang M., Yu S.W, & Wang L. (2020). Myricetin induces apoptosis and autophagy by inhibiting PI3K/Akt/mTOR signalling in human colon cancer cells. BMC Complementary Medicine and Therapies, 20, 209.

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Ultrahigh Performance Liquid Chromatography for Flavonoid Analysis

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Methanol and acetonitrile used in ultrahigh performance liquid chromatography (UHPLC) were purchased from OmniGene LLC (Morrisville, NC, USA). Analytical flavonoid standards, i.e., catechin, myricetin, epicatechin, rutin, quercetin, liquiritigenin, cyanidin-3-O-glucoside, gallic acid, Trolox, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), quercetin-7-O-β-D-glucopyranoside, and 2,4,6-tri-(2-pyridyl)-1,3,5-triazine (TPTZ) were purchased from Solarbio Technology Co. Ltd. (Beijing, China).

Fu H., Qiao Y., Wang P., Mu X., Zhang J., Fu B, & Du J. (2021). Changes of bioactive components and antioxidant potential during fruit development of Prunus humilis Bunge. PLoS ONE, 16(5), e0251300.

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