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4 protocols using ethyl vanillate

1

Analytical Standards for Flavor and Fragrance Research

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Octan-2-ol (97%), 1-hexanol (99%), cis-3-hexenol (98%), trans-3-hexenol (97%), vanillin (99%), 2,6-dimethoxyphenol (99%), linalool (97%), terpinen-4-ol (≥95%), α-terpineol (90%), nerol (≥97%), geraniol (98%), linalool oxide (≥97%), β-citronellol (95%), p-cymene (99%), terpinolene (≥85%), γ-terpinene (≥97%), limonene (97%), 1,8-cineole (99%), 1,4-cineole(≥98.5%), β-damascenone (≥98%), isoamyl alcohol (98%), benzyl alcohol (≥99%), 2-phenylethanol (≥99%), ethyl acetate (99%), ethyl butanoate (99%), ethyl 3-methyl butanoate (≥98%), isoamyl acetate (≥95%), ethyl hexanoate (≥95%), phenylethyl acetate (99%), n-hexyl acetate (≥98%), ethyl lactate (≥98%), ethyl octanoate (≥98%), ethyl decanoate (≥98%), hexanoic acid (≥99%), octanoic acid (≥98%), α-phellandrene (95%), p-menthane-1,8-diol (97%), 3-methylbutanoic acid (99%), α-ionone (90%), 1-pentanol (99%), 1-butanol (≥99%), 2-butanol (≥99%), ethyl guaiacol (≥99%), vinyl guaiacol (≥98%), methyl-vanillate (99%), ethyl vanillate (99%), were supplied by Sigma Aldrich (Milan, Italy). Dichloromethane (≥99.8%) and methanol (≥99.8%), were provided by Honeywell (Seelze, Germany). Sodium chloride (≥99.5%) was supplied by Sigma Aldrich (Milan, Italy).
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2

Quantification of Bioactive Compounds

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The following compounds of the purity ≥98% were purchased from Sigma-Aldrich (Saint Louis, MO, USA): ethyl 4-hydroxybenzoate (E-4-OH-BA), ethyl vanillate (EV), gallic acid (GA), gentisic acid (GNA), homogentisic acid (HGA), homovanillic acid (HVA), 3-hydroxybenzoic acid (3-OH-BA), 4-hydroxybenzoic acid (4-OH-BA), 4-hydroxyphenylacetic acid (4-OH-PA), 4-hydroxyphenylpyruvic acid (4-OH-PP), nordihydroguaiaretic acid (NDGA), protocatechuic acid (PCA), salicylic acid (SA), sinapic acid (SNA), syringic acid (SRA), vanillic acid (VA), acetylthiocholine iodide (ACh), and Acetylcholinesterase from Electrophorus electricus (electric eel) (AChE). Methyl syringate (MS) was purchased from Apin Chemicals Ltd., (Abingdon, UK).
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3

Comprehensive Aroma Compound Library Characterization

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Octan-2-ol (97%), 1-hexanol (99%), cis-3-hexenol (98%), trans-3-hexenol (97%), vanillin (99%), 2,6-dimethoxyphenol (99%), linalool (97%), terpinen-4-ol (≥95%), α-terpineol (90%), nerol (≥97%), geraniol (98%), linalool oxide (≥97%), β-citronellol (95%), p-cymene (99%), terpinolene (≥85%), γ-terpinene (≥97%), limonene (97%), 1,8-cineole (99%), 1,4-cineole(≥98.5%), β-damascenone (≥98%), isoamyl alcohol (98%), benzyl alcohol (≥99%), 2-phenylethanol (≥99%), ethyl acetate (99%), ethyl butanoate (99%), ethyl 3-methyl butanoate ((≥98%), isoamyl acetate (≥95%), ethyl hexanoate (≥95%), phenylethyl acetate (99%), n-hexyl acetate (≥98%), ethyl lactate (≥98%), ethyl octanoate (≥98%), ethyl decanoate (≥98%), hexanoic acid (≥99%), octanoic acid (≥98%), α-phellandrene (95%), p-menthane-1,8-diol (97%), 3-methylbutanoic acid (99%), α-ionone (90%), 1-pentanol (99%), 1-butanol (≥99%), 2-butanol (≥99%), ethyl guaiacol (≥99%), vinyl guaiacol (≥98%), methyl-vanillate (99%) and ethyl vanillate (99%), were supplied by Sigma Aldrich (Milan, Italy). Dichloromethane (≥99.8%) and methanol (≥99.8%), were provided by Honeywell (Seelze, Germany). Sodium chloride (≥99.5%) was supplied by Sigma Aldrich (Milan, Italy).
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4

Quantification of Phenolic Compounds

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Ethyl gallate, 4-hydroxybenzaldehyde, vanillin, tyrosol, protocatechuic aldehyde, syringaldehyde, pyrogallol, 4-hydroxy-3-methoxycinnamaldehyde, 4-methylcatechol, tryptophol, ethyl 3,4-dihydroxycinnamate, 2,6-dimethoxyphenol, ethyl vanillate, homovanillyl alcohol, eugenol, 4-hydroxy-3-methoxyphenylacetone, salicylaldehyde, coniferyl alcohol, epicatechin, catechin, scopoletin, p-coumaric acid, caffeic acid, resveratrol, syringic acid, rutin, gallic acid, salicylic acid, protocatechuic acid, 2-methoxy-4-vinylphenol, 4-vinylphenol, 4-ethylguaiacol were purchased from Sigma-Aldrich (Steinheim, Germany). All chemicals were used in the highest purity available. Acetonitrile and methanol (gradient grade for liquid chromatography) were also purchased from Sigma-Aldrich. Deionized water was produced by Mili-Q® Reference System (Merck KGaA, Darmstadt, Germany). Sodium chloride was purchased by Lach-Ner (Neratovice, Czech Republic). Standard solution of n-alkanes C8-C33 was purchased by CPAchem (Stara Zagora, Bulgaria). Technical gases, i.e., helium 4.6, hydrogen, and nitrogen, were purchased from Linde Gas (Prague, Czech Republic).
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