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Ascorbic acid vc

Manufactured by Sinopharm
Sourced in China

Ascorbic acid, also known as vitamin C, is a water-soluble essential nutrient commonly used in laboratory settings. It is a reducing agent and acts as an antioxidant, playing a crucial role in various enzymatic reactions. Ascorbic acid is an important compound for researchers and scientists working in fields such as biochemistry, cell biology, and analytical chemistry.

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8 protocols using ascorbic acid vc

1

Antioxidant Compounds Characterization

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Chlorogenic acid, isoorientin, and apigenin were purchased from Shanghai Natural Biological Technology Co., Ltd. (Shanghai, China). Iso-vitexin, cynaroside, and luteolin were obtained from Shanghai Yuanye Biological Technology Co., Ltd. (Shanghai, China). Orientin was bought from Chengdu Push Biological Technology Co., Ltd. (Chengdu, China). The purity of all standards was more than 98%, and their chemical structures are listed in Figure 5. DPPH was gained from Sigma-Aldrich (St. Louis, MO, USA). Trolox was from Beyotime Institute of Biotechnology Co., Ltd. (Shanghai, China). Ascorbic acid (VC) was ordered from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Diamonsil Plus C18 column (4.6 × 250 mm, 5 µm) was used. All liquid chromatography solvents (methanol, acetonitrile) were of HPLC grade, obtained from Anhui Fulltime Specialized Solvent and Reagent Co., Ltd. (Anqing, China), and used after filtration by a 0.45 µm organic membrane. All other solvents were of analytical grade and used without further purification.
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2

Synthesis and Characterization of AgNWs

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The
following chemicals,
solvents, and materials were used to prepare AgNWs, AgNW ink, and
AgNW-based TCFs, which all were analytical grade reagents and used
as received without any further purification. Silver nitrate (AgNO3, ≥99.8%, Strem Chemicals), ascorbic acid (Vc, ≥99.7%,
Sinopharm), PVP (Mw ≈ 1,300,000,
Aldrich), sodium chloride (NaCl, ≥99.5%, Sinopharm), EG (≥99.0%,
Sinopharm), hydroxyethyl cellulose (HEC, Dow-QP-100MH), ethanol (C2H5OH, ≥99.0%, Xilong Chemicals), and polyethylene
glycol terephthalate (PET, Yingshang Electronic Material) were all
purchased from commercial sources. Deionized water was obtained through
a purification system with a resistivity of 18.2 MΩ·cm.
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3

Antioxidant Compounds Characterization

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Sodium dodecyl benzene sulfonate (SDBS, analytical purity) was acquired from Wuxi Ya Tai United Chemical Co., Ltd. (Jiangsu, PR China). 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) and 2,2′-azinobis-(3-ethyl benzthiazoline-6-sulfonic acid) (ABTS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ascorbic acid (Vc) and butylated hydroxy toluene (BHT) were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
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4

Comprehensive Phytochemical Analysis Protocol

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Sodium acetate, sodium citrate buffer and ascorbic acid (Vc) were purchased from Sinopharm Chemical Reagent Co (Shanghai, China). Methanol and acetonitrile (MS and HPLC grade) were purchased from Fisher Scientific (England). 2,2-Diphenylpicrylhydrazyl (DPPH), nicotinamide adenine dinucleotide disodium salt (NADH-2Na), ammonium acetate (NH4AC), ammonium hydroxide (NH4OH), undecanoic acid, amino acid standards (19 L-amino acids, including Ala, Gly, Val, Leu, Ile, Phe, Met, Pro, Ser, Thr, Cys, Tyr, Asn, Gln, His, Lys, Arg, Asp., Glu), and minerals (including calcium (Ca), copper (Cu), iron (Fe), potassium (K), sodium (Na), magnesium (Mg), manganese (Mn), phosphorus (P), selenium (Se), and zinc (Zn)), and Folin–Ciocalteu reagent were obtained from Sigma–Aldrich (Shanghai, China). Hyperoside (98%), emodin (98%), rhein (98%), aloe-emodin (98%), myricitrin (98%), kaempferol (98%), isoorientin (98%), and gallic acid (98%) were purchased from Yuanye Co. (Shang, China) and Macklin Co. (Shang, China), respectively.
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5

Extraction and Characterization of Amaranthus Shoots

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Amaranthus hybridus was obtained from Sichuan Agricultural University and identified by Professor Hui Chen. The materials were placed in the shade to dry and stored at room temperature. In the study, the shoots of A. hybridus was used as material. The mature stage of A. hybridus was used for experimentation. The plants were cultivated on the farm of Sichuan Agricultural University. They were sown in April and harvested in September. Nitro blue tetrazolium (NBT), KBr (spectroscopically pure) and a dextran standard were purchased from Sigma (St. Louis, MO, USA). 2,2-diphenyl-1-picrylhydrazyl (DPPH) was purchased from Yuanye Co. (Shanghai, China), Ascorbic acid (Vc) was purchased from Sinopharm Chemical Reagent Co. (Shanghai, China), diethylaminoethyl cellulose-32 (DEAE) was purchased from Whatman Co. (Maidstone, Kent, UK), Sephadex G-100 was purchased from Pharmacia Co. (New York, NY, USA). All other chemicals and reagents were analytical grade.
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6

Antioxidant and Cholinesterase Inhibition Assays

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Folin–Ciocalteu reagent, salicylic acid, Na2CO3, ascorbic acid (Vc), ethanol, H2O2 solution (30%, w/w), and other reagents (analytical grade) were acquired from Sinopharm Chemical Reagent Co., Ltd. (Chengdu, China). Gallic acid, DPPH, ABTS, 2,4,6-Tris(2-pyridyl)-S-triazine (TPTZ), ACHE from electric eel, BuChE from equine serum, and donepezil hydrochloride were obtained from Yuan Ye Biological Technology Company Ltd. In Shanghai, China.
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7

Antioxidant Properties of Black Raspberry

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Black raspberry was purchased from Blackberry Seedlings Cooperatives, Jilin, China. The fresh berries were washed, freeze dried under vacuum, and crushed into powder. 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), and Folin–Ciocalteu reagent were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ascorbic acid (VC) and polyamide resin were purchased from Sinopharm Chemical Reagent Co., Ltd. Cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside (>90% by HPLC) were isolated in our previous work. Fetal bovine serum, Dulbecco’s Modified Eagle’s medium (DMEM), and penicillin-streptomycin were purchased from Thermo Fisher Scientific Co., Ltd. (St. Wyman, MA, USA). MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) reagent, Mitomycin C and Silymarin were acquired from Sigma-Aldrich (St. Louis, MO, USA). Acetonitrile, methanol and formic acid of HPLC grade were purchased from Fisher Scientific (Pittsburgh, PA, USA).
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8

Bioactive Compounds from D. huoshanense

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Wild plants of D. huoshanense growing in Huoshan County, Anhui Province, China, were collected. Dimethyl sulfoxide (DMSO), acetonitrile, sodium hydride (NaH), and iodomethane (CH3I) lipopolysaccharide (LPS) were purchased from Sigma Chemical Co., USA. DEAE-cellulose and Sephadex G-100 were purchased from Solarbo Co., Ltd. (Beijing, China). The Cell counting kit-8 (CCk-8) was purchased from Han Heng Biotechnology Co., Ltd. (Shanghai, China). The ascorbic acid (VC) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All the other reagents or chemicals were of analytical grade.
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