The miR-23b-3p mimic, inhibitor, and corresponding controls were purchased from GenePharma (Shanghai, China). c-MET siRNA and negative control (NC) were purchased from Dharmacon (Lafayette, CO, USA). SNU-354 and SNU-368 cells were transiently transfected with Lipofectamine RNAiMAX (Invitrogen, Carlsbad, CA, USA) according to the instructions provided by the manufacturer. After transfection for 48 h, the cells were harvested, and the transfection efficiency was analyzed by qRT-PCR.
Mir 23b 3p mimic
Manufactured by GenePharma
Sourced in China
The MiR-23b-3p mimics are a type of lab equipment designed to simulate the function of the microRNA miR-23b-3p. MicroRNAs are small non-coding RNA molecules that play a regulatory role in gene expression. The MiR-23b-3p mimics can be used in research applications to study the biological functions and target genes of this specific microRNA.
Automatically generated - may contain errors
Lab products found in correlation
C met sirna, by Horizon Discovery (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
Mir 23b 3p inhibitor, by GenePharma (1 mentions)
Rnaimax, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Ly294002, by Merck Group (1 mentions)
Lentiviral vectors expressing, by GenePharma (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000 kit, by Thermo Fisher Scientific (1 mentions)
5 protocols using mir 23b 3p mimic
1
miR-23b-3p Mimics and Inhibitors
2
Modulating miR-23b-3p in Osteosarcoma
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MiR-23b-3p inhibitor, miR-23b-3p mimic, and miRNA negative control (NC) of miR-23b-3p were purchased from GenePharma. The HOS and U2OS cells were transfected with 40 nM MiR-23b-3p inhibitor, miR-23b-3p mimic, NC, or VEPH1-OE vectors using Lipofectamine 3000 and RNAiMax (Life Technologies, USA) according to the manufacturer’s instructions. Transfection efficiency was confirmed by reverse transcription quantitative (RT-q) PCR 48 h after cell transfection. The primer sequences used are listed in Table S1. To block the PI3K/AKT signaling pathway, HOS and U2OS cells were treated with 10 μM LY294002 (the PI3K/AKT pathway inhibitor; Sigma-Aldrich, USA) for 48 h [34 (link)].
3
Lentiviral Overexpression and Knockdown of PGC1α and miR-23b-3p
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Lentiviral vectors expressing PGC1α, miR‑23b-3p shRNA, PGC1α shRNA, and control construct were purchased from GenePharma (Shanghai, China). Stable cell lines overexpressing PGC1α, PGC1α shRNA, or/and miR‑23b-3p shRNA were established by infection with the lentiviruses respectively with polybrene (GM-040901B, Genomeditech, Shanghai, China) according to the manufacturer’s instructions. The infected cells were selected using puromycin or blasticidin. miR-23b-3p mimics were purchased from GenePharma (Shanghai, China).
4
Overexpression Plasmids Transfection
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The LINC00261‐overexpression plasmids (pcDNA3.1‐LINC00261), FOXO3a‐overexpression plasmids (pcDNA3.1‐FOXO3a), NRF2‐overexpression plasmids, vector controls and miR‐23b‐3p mimics were synthesized and purchased from GenePharma. Lipofectamine 2000 (Invitrogen) was used to transfect the cells according to the manufacturer's protocol.
5
NEAT1 and miR-23b-3p in hBMSCs
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The overexpression plasmids, siRNAs and miR-23b-3p mimics were constructed by Shanghai GenePharma Co., Ltd. (China). The groups were as follows: si-NC group (transfected with negative control of knockdown gene), si-NEAT1 group (transfected with NEAT1 siRNA), OE-NC group (transfected with negative control of overexpression plasmid), OE-NEAT1 group (transfected with NEAT1 overexpression plasmid), OE-NEAT1 + miR-NC group (transfected with NEAT1 overexpression plasmid and injected with miRNA negative control), OE-NEAT1 + miR-23b-3p mimic group (transfected with NEAT1 overexpression plasmid and injected with miR-23b-3p mimic), miR-NC group (injected with miRNA negative control), miR-23b-3p mimic group (injected with miR-23b-3p mimic), miR-23b-3p mimic + NC group (injected with miR-23b-3p mimic and transfected with negative control of overexpression plasmid) and miR-23b-3p mimic + OE-CYP1A2 group (injected with miR-23b-3p mimic and transfected with CYP1A2 overexpression plasmid). The plasmids and siRNAs were transfected into hBMSCs by Lipofectamine 3000 kit (L3000001; Thermo Fisher Scientific, USA). Forty-eight hours after transfection, the experiments below were carried out.
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