Sorted naïve CD4+CD62L+Foxp3EGFP− T cells (1×106/ml) were cultured with plate-bound anti-CD28 (5μg/ml, Biolegend), anti-CD3 (5μg/ml, Biolegend), recombinant TGF-β Sigma-Aldrich) and in the presence of a gradient of recombinant IL-4 (Perpotech). After 3 days, the induced iTreg cells were analyzed by flow cytometry and/or re-sorted based upon EGFP fluorescence.
Recombinant tgf β
Manufactured by Merck Group
Sourced in United States
Recombinant TGF-β is a laboratory reagent used in research applications. It is a growth factor protein produced through recombinant DNA technology. TGF-β plays a role in regulating cellular processes such as cell growth, differentiation, and immune function.
Automatically generated - may contain errors
2 protocols using recombinant tgf β
1
Induction of Regulatory T Cells from Naive CD4+ Cells
2
TGF-β Inhibitor Modulates NGF in IVD Cells
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To determine whether the TGF‐β inhibitor, SB431542, inhibits Ngf expression and NGF production in IVD cells, we examined the effect of SB431542 on TGF‐β-mediated Ngf expression and NGF production by IVD cells isolated from five mice. IVD cells were isolated using collagenase digestion, as described above. Disc cells were subsequently incubated in α‐minimal essential media (α‐MEM) with 10% fetal bovine serum in six‐well plates. One week later, IVD cells were stimulated with α‐MEM (vehicle), 10 ng/ml recombinant TGF‐β (Product no. 7666-MB, R&D Systems, Minneapolis, MN, USA), or 10 ng/ml recombinant TGF‐β + 1 µM SB431542 (Product no. S4317, Sigma Aldrich, St Louis, MO, USA) for 6 and 24 h. Thereafter, total mRNA was extracted and analyzed using qPCR. NGF concentration in the cell supernatant was determined using a commercial NGF ELISA kit (Product No. DY556, R&D Systems).
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