Rabbit polyclonal antibodies against iκb α

z-VAD-fmk (pan-caspase inhibitor) and calpeptin (calpain inhibitor) were purchased from EMD Biosciences (Darmstadt, Germany). Staurosporin was purchased from Sigma Chemical Company (St. Louis, Missouri, USA). Rabbit polyclonal antibodies against cleaved caspase-3, NF-κB (p65), STAT3, STAT5, and β-actin were purchased from Cell Signaling Technology (Beverly, Massachusetts, USA). Rabbit polyclonal antibodies against IκB-α were purchased from Santa Cruz (Delaware, California, USA). Rabbit polyclonal antibodies against m-calpain and mouse monoclonal antibodies against the calpain regulatory subunit and calpastatin were purchased from Calbiochem (La Jolla, California, USA). Unless stated otherwise, all other reagents were purchased from Sigma-Aldrich (St. Louis, Missouri, USA).

Recombinant human M-CSF and mouse RANKL proteins were prepared as previously described⁵ (link). BA was purchased from AK Scientific, Inc. (Union City, CA, USA) and recombinant human IL-1β was supplied by R&D systems, Inc. (Minneapolis, MN, USA). PGE2 was purchased from Cayman Chemical (Ann Arbor, MI, USA). Lipopolysaccharide (LPS), U0126 and SC-514 were supplied by MilliporeSigma (Burlington, MA, USA). SB202190 and SP600125 were supplied by AG Scientific, Inc. (San Diego, CA, USA). A rabbit polyclonal antibody against β-actin was purchased from Abcam (Cambridge, MA, USA). Rabbit polyclonal antibodies against phosphorylated (p-)IκBα, p-JNK, p-ERK and p-P38, and a rabbit monoclonal antibody against p-p65 were purchased from Cell Signaling Technology (Danvers, MA, USA). A goat polyclonal anti-mouse secondary antibody (Alexa Fluor 546 conjugate) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Rabbit polyclonal antibodies against IκBα, JNK1, P38 and c-FOS, and mouse monoclonal antibodies against NFATc1, p65, ERK2, CTSK and integrin β₃ were procured from Santa Cruz Biotechnology (Dallas, TX, USA). A rabbit polyclonal antibody against superoxide dismutase 2 (SOD2) was purchased from Upstate Biotechnology (Lake Placid, NY, USA). A rabbit monoclonal antibody against ATP6V0D2 was kindly provided by Dr. SY Lee (Ewha Womans Univ. Seoul, Korea).