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Alexa flour 647 azide

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Alexa Fluor 647-azide is a fluorescent dye conjugate used for labeling biomolecules. It has an excitation maximum at 650 nm and an emission maximum at 665 nm, making it suitable for detection in the red/far-red region of the visible spectrum.

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Lab products found in correlation

Click it imaging kit, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Sab4200236, by Merck Group (1 mentions) Pepstatin a, by Avantor (1 mentions) 3xflag peptide, by Merck Group (1 mentions) Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions) Gapdh, by Merck Group (1 mentions) Anti flag m2 affinity gel, by Merck Group (1 mentions) Aprotinin, by Merck Group (1 mentions) Leupeptin, by Merck Group (1 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by MP Biomedicals (1 mentions) Click it imaging kit with alexa flour 488 azide, by Thermo Fisher Scientific (1 mentions)

4 protocols using alexa flour 647 azide

1

Evaluating Cell Proliferation in Mice

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To evaluate cell proliferation, 1mg of 5-ethynyl-2’-deoxyuridine (EdU) (Invitrogen A10044) dissolved in PBS was administered to pregnant mice three hours before sacrificed at indicated embryonic days. Click-iT Imaging Kit (Invitrogen, C10337) with Alexa Flour 647-azide (Invitrogen A10277) was used to detect EdU in cryosections.
Ono N., Ono W., Nagasawa T, & Kronenberg H.M. (2014). A Subset of Chondrogenic Cells Provides Early Mesenchymal Progenitors in Growing Bones. Nature cell biology, 16(12), 1157-1167.
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2

Evaluating Cell Proliferation in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
To evaluate cell proliferation, 1mg of 5-ethynyl-2’-deoxyuridine (EdU) (Invitrogen A10044) dissolved in PBS was administered to pregnant mice three hours before sacrificed at indicated embryonic days. Click-iT Imaging Kit (Invitrogen, C10337) with Alexa Flour 647-azide (Invitrogen A10277) was used to detect EdU in cryosections.
Ono N., Ono W., Nagasawa T, & Kronenberg H.M. (2014). A Subset of Chondrogenic Cells Provides Early Mesenchymal Progenitors in Growing Bones. Nature cell biology, 16(12), 1157-1167.
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3

Affinity Purification and Labeling Protocol

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Anti-FLAG M2 affinity gel and 3X FLAG peptide were purchased from Sigma. A 1000x protease inhibitor cocktail of 5 mg/ml leupeptin (Sigma), 3 mg/ml aprotinin (Sigma), 1 M PMSF (MP Biomedicals) and 1 mM pepstatin A (Amresco) was mixed from individual components. Alexa Flour647 azide was purchased from Invitrogen. [3H]-palmitoyl CoA was synthesized as previously described (45 (link)). HEK-293 cells were cultured in Dulbecco’s modified Eagle medium (DMEM) (Gibco) media with 10% fetal bovine serum (Life Technologies). Cell were maintained at 37 °C in a humidified incubator supplemented with 5% CO2. Polyethylenimine (PEI) (25k, linear) was purchased from Polysciences and was dissolved in water to make a 1000x stock (1 mg/ml). The following commercial antibodies were used: M2 FLAG (Sigma-Aldrich, F1804), MBLAC2 (Sigma-Aldrich, HPA037757), GFP (Invitrogen, A-11122), ER marker, calnexin (Cell Signaling, C5C9), nuclear marker, lamin (Sigma-Aldrich, SAB4200236), cytosolic marker, GAPDH (Sigma, G8795).
Malgapo M.I., Safadi J.M, & Linder M.E. (2020). Metallo-β-lactamase domain-containing protein 2 is S-palmitoylated and exhibits acyl-CoA hydrolase activity. The Journal of Biological Chemistry, 296, 100106.
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4

EdU-based Cell Proliferation Assay

Cited 2 times
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To evaluate cell proliferation, 5-ethynyl-2’-deoxyuridine (EdU) (Invitrogen A10044) dissolved in PBS was administered to mice at the indicated postnatal days 3 hours before sacrifice. Click-iT Imaging Kit with Alexa Flour 488-azide (Invitrogen, C10337) or Alexa Flour 647-azide (A10277) was used to detect EdU in cryosections15 (link),48 (link).
Ma C., Jing Y., Li H., Wang K., Wang Z., Xu C., Sun X., Kaji D., Han X., Huang A, & Feng J. (2020). ScxLin cells directly form a subset of chondrocytes in temporomandibular joint that are sharply increased in Dmp1-null mice. Bone, 142, 115687.
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