Sw620

The human colorectal cancer (CRC) cell lines of SW480, SW620 and HCT116 were obtained from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. and cultured in DMEM medium (Gibco, USA) supplemented with 10% fetal bovine serum (BI, USA) at 37 °C in 5%CO₂. In order to evaluate the influence of NF-κB pathway on the migration, invasion and EMT of CRC cells, cells were treated with NF-κB pathway inhibitor BAY 11–7082 (10 μM, medchemexpress, USA) for 48 h before following experiments.

CC cells, including SW480, Colo-205, SW620, HCT15, DLD-1, HCT116, Caco-2, RKO and LOVO were bought from Shanghai Zhong Qiao Xin Zhou Biotechnology. SW480 and SW620 were cultured in a complete DMEM medium supplemented with 10% FBS (Gibco Grand Island, NY, USA). Colo-205, HCT15 and DLD-1 cells in a complete RPMI-1640 medium supplemented with 10% FBS. Caco-2 and RKO cells in a complete EMEM medium supplemented with 10% FBS. HCT116 cells in a complete Mccoy’s 5A medium supplemented with 10% FBS. LOVO cells in a complete F-12K medium supplemented with 10% FBS. All the experimental procedures described below were repeated thrice unless described otherwise.

Human CRC cell lines SW480, SW620, HT29, Colo205, Caco-2 and HCT116 were purchased from Shanghai Zhong Qiao Xin Zhou Biotechnology Co.,Ltd (Shanghai, China). Caco-2, SW620 and Colo205 cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), HCT116 cells were cultured in DMEM medium supplemented with 10% FBS, HT29 were cultured in McCoy’s 5A medium supplemented with 10% FBS, SW480 were cultured in Leibovitzs L-15 medium supplemented with 10% FBS. All types of culture mediums were purchased from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. All CRC cells were maintained in an incubator under the condition of 5% CO2 at 37°C.