MeCP2tm1.1Bird+/− female mice (Mecp2+/−) were purchased from the Jackson Laboratory (ME, USA) (Jax stock number: 003890) and mated with C57BL/6 wild-type male mice (Jax stock number: 000664). Wild-type female littermates served as controls (WT Eleven-month-old WT and RTT mice were euthanized via cervical dislocation under anesthesia, and hearts were rapidly dissected, washed in 1 × PBS to remove blood, and snap frozen in liquid nitrogen. National or institutional guidelines were applied in the care and use of animals, and all experimental protocols were approved by the Ethics Committee of the Second University of Naples.
Mecp2tm1.1bird
Manufactured by Jackson ImmunoResearch
Sourced in United States
Mecp2tm1.1Bird is a laboratory mouse model with a targeted deletion of the Mecp2 gene. The Mecp2 gene encodes the methyl-CpG-binding protein 2, which is important for neural development and function. This model is used to study the role of Mecp2 in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Mecp2, by Jackson ImmunoResearch (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Minimal essential medium, by Thermo Fisher Scientific (1 mentions)
Eagle s minimal essential medium, by Thermo Fisher Scientific (1 mentions)
Sk n sh, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Wisent (1 mentions)
Mecp2 y, by Jackson ImmunoResearch (1 mentions)
C57bl 6j, by Jackson ImmunoResearch (1 mentions)
4 protocols using mecp2tm1.1bird
1
Aging Female RTT Mice Heart Study
2
Culturing Human Neuroblastoma and Mouse Fibroblasts
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Human neuroblastoma cell line SK-N-SH (ATCC, Manassas, VA, USA) was grown in Eagle’s Minimal Essential Medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% FBS (Wisent, St. Bruno, QC, Canada) and Penicillin-Streptomycin (Invitrogen, Carlsbad, CA, USA) Primary mouse fibroblasts isolated from MECP2 knockout mice Mecp2tm1.1Bird (Jackson laboratories, ME, USA) were generated in the Ellis lab by standard procedures, and were grown in Minimal Essential Medium (Invitrogen, Carlsbad, CA, USA) supplemented with 20% FBS and Penicillin-Streptomycin. Cells were kept at 37°C in humid atmosphere with 5% CO2.
3
MECP2-deficient Mice Experiments
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The animal experiments were performed at the Center of Animal Care and Use (CACU), Lee Gil Ya Cancer and Diabetes Institute of GACHON University. Five-week-old MECP2-deficient male mice (Mecp2–/y) (The Jackson Laboratory, stock no: 003890, Mecp2TM 1.1Bird) and 5-week-old male C57BL/6N mice were randomly divided and housed with five mice per cage in a temperature-controlled environment with a 12 h light/dark cycle. The mice had free access to food and water. All animal protocols described in this study were approved by the CACU Animal Center Ethics Board (LCDI-2018-008).
4
Murine Models of Rett Syndrome
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All rat experiments were carried out in accordance with the European Communities Council Directive (86/609/EEC) and with the terms of a project license under the UK Scientific Procedures Act (1986). The Mecp2-/y rats were maintained by crossing Mecp2+/− females (SD-Mecp2em1Sage) with wild type Sprague Dawley males. Animals were maintained on 12-h light/dark cycles with free access to normal rat food and water. WT and Mecp2-/y rats at 25 days of postnatal age were weighed and assessed for the development of the RTT-like phenotypes prior to surgery.
Mouse animal husbandry and euthanasia was carried out as approved by the Emory University Institutional Animal Care and Use Committees. Male and female mice of C57BL/6J, Mecp2 deficient (Mecp2tm1.1Bird), and Cdkl5-deficient (Cdkl5tm1.1Joez) were obtained from the The Jackson Laboratory stocks (The Jackson Laboratory #000664, #003890 and #021967, respectively). All animals were of 6 weeks of age. Animals were maintained on 12-h light/dark cycles with free access to mouse chow and water.
Mouse animal husbandry and euthanasia was carried out as approved by the Emory University Institutional Animal Care and Use Committees. Male and female mice of C57BL/6J, Mecp2 deficient (Mecp2tm1.1Bird), and Cdkl5-deficient (Cdkl5tm1.1Joez) were obtained from the The Jackson Laboratory stocks (The Jackson Laboratory #000664, #003890 and #021967, respectively). All animals were of 6 weeks of age. Animals were maintained on 12-h light/dark cycles with free access to mouse chow and water.
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