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Bioanalyser nano kit

Manufactured by Agilent Technologies
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The Bioanalyser Nano Kit is a lab equipment product from Agilent Technologies. It is designed for the analysis of small sample volumes, such as DNA, RNA, and protein samples. The core function of the Bioanalyser Nano Kit is to provide high-resolution electrophoretic separation and sensitive detection of these biomolecules.

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Lab products found in correlation

Trizol, by Thermo Fisher Scientific (3 mentions) Agilent 2100 bioanalyser, by Agilent Technologies (3 mentions) Rneasy mini kit, by Qiagen (2 mentions) Rnase free dnase, by Promega (2 mentions) Superscript 3 first strand synthesis system, by Thermo Fisher Scientific (2 mentions) Taqman universal pcr master mix kit, by Thermo Fisher Scientific (1 mentions) Abi 7500 fast real time pcr system, by Thermo Fisher Scientific (1 mentions) Rneasy plant mini kit, by Qiagen (1 mentions) Dnase, by Promega (1 mentions)

Close spelling variants of this product

Bioanalyser (232 citations) Bioanalyser RNA6000 Nano kit (3 citations) Agilent 2100 Bioanalyser RNA 6000 Nano kit (2 citations) Bioanalyser RNA Nano 6000 kit (2 citations) Agilent 2100 Bioanalyser (Agilent RNA 6000 Nano Kit (2 citations)

3 protocols using bioanalyser nano kit

1

RNA Extraction and Purification from Mycelia

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Mycelia were harvested by vacuum filtration and immediately frozen in liquid nitrogen. Mycelia were ground to a fine powder under liquid nitrogen and total RNA was extracted using TRIZOL, according to manufacturer’s instructions (Invitrogen), before being treated with RNAse-free DNAse (Promega) and purified with the RNeasy® Mini Kit, according to manufacturer’s instructions (Qiagen). RNA integrity was confirmed using the Bioanalyser Nano Kit (Agilent Technologies) and the Agilent Bioanalyser 2100, considering RIM value 8.0 as the RNA quality threshold. The SuperScript III First Strand Synthesis system (Invitrogen) and oligo(dT) primers were used for cDNA synthesis according to manufacturer’s instructions. All the primer sequences used in this work are described in the Additional file 5: Table S4.
de Assis L.J., Ries L.N., Savoldi M., dos Reis T.F., Brown N.A, & Goldman G.H. (2015). Aspergillus nidulans protein kinase A plays an important role in cellulase production. Biotechnology for Biofuels, 8, 213.
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2

Transcriptome Analysis of Fungal Metabolism

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Mycelia were harvested by vacuum filtration and immediately frozen in liquid nitrogen. Mycelia were ground into a fine powder in liquid nitrogen and the total RNA was extracted using TRIZOL (Invitrogen) before being treated with RNAse-free DNAse (Promega) and purified with the RNeasy Mini Kit (Qiagen). RNA integrity was confirmed using the Bioanalyser Nano Kit (Agilent Technologies) and the Agilent Bioanalyser 2100. The SuperScript III First Strand Synthesis system (Invitrogen) and oligo(dT) primers were used for cDNA synthesis according to the manufacturer’s instructions. All RT-qPCR reactions were performed using an ABI 7500 Fast Real-Time PCR System and Taq-Man Universal PCR Master Mix kit (Applied Biosystems). The tubulin gene tubC was used as an endogenous control. The primers used for the glucose transporter and metabolic enzyme encoding genes are listed in Supporting Information, Table S1.
de Assis L.J., Ries L.N., Savoldi M., Dinamarco T.M., Goldman G.H, & Brown N.A. (2015). Multiple Phosphatases Regulate Carbon Source-Dependent Germination and Primary Metabolism in Aspergillus nidulans. G3: Genes|Genomes|Genetics, 5(5), 857-872.
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3

Fungal Transcriptome Extraction and Analysis

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Fungal biomass was harvested at the presented intervals and immediately frozen in liquid nitrogen. Total RNA was extracted using TRIzol (Invitrogen), treated with DNAse (Promega) and purified using RNeasy Plant Mini Kit (Qiagen). RNA integrity was confirmed using the Bioanalyser Nano kit (Agilent technologies) and the Agilent Bioanalyser 2100.
Brown N.A., Ries L.N., Reis T.F., Rajendran R., Corrêa dos Santos R.A., Ramage G., Riaño-Pachón D.M, & Goldman G.H. (2016). RNAseq reveals hydrophobins that are involved in the adaptation of Aspergillus nidulans to lignocellulose. Biotechnology for Biofuels, 9, 145.
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