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Raybio human chemokine antibody array 1

Manufactured by RayBiotech
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The RayBio Human Chemokine Antibody Array 1 is a multiplex assay that allows for the simultaneous detection and quantification of 41 different human chemokines in a single sample. The array consists of capture antibodies specific to each chemokine, immobilized on a solid support. The sample is incubated with the array, and the bound chemokines are detected using a cocktail of biotinylated detection antibodies and a streptavidin-based signal amplification system.

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Lab products found in correlation

Raybio human cytokine antibody array 5, by RayBiotech (1 mentions)

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Antibody arrays

2 protocols using raybio human chemokine antibody array 1

1

Cytokine Detection in Conditioned Media

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The presence of soluble factors in the CM were detected using the RayBio Human Chemokine Antibody Array 1 (Cat# AAH-CHE-1) for tumoral CM and RayBio Human Cytokine Antibody Array 5 (Cat# AAH-CYT-5) for MSC CM (Ray Biotech, Inc.) according to the manufacturer's protocol. The intensities of signals were quantified by densitometry with ImageJ software (National Institute of Health, USA) and positive controls were used to normalize the results from different membranes.
Bayo J., Real A., Fiore E.J., Malvicini M., Sganga L., Bolontrade M., Andriani O., Bizama C., Fresno C., Podhajcer O., Fernandez E., Gidekel M., Mazzolini G.D, & García M.G. (2016). IL-8, GRO and MCP-1 produced by hepatocellular carcinoma microenvironment determine the migratory capacity of human bone marrow-derived mesenchymal stromal cells without affecting tumor aggressiveness. Oncotarget, 8(46), 80235-80248.
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2

Lentiviral Gene Silencing in BM-MSCs

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A lentiviral system obtained from Taiwan National RNAi Core Facility was used for gene silencing in BM-MSCs [28] (link). Lentivirus-infected cells (5×104) were cultured in fresh growth medium (IMDM supplemented with 10% FBS, 1% l-glutamine, 0.1% penicillin–streptomycin, and 10 ng/ml bFGF) in a 6-cm tissue culture plate for 3 days. Culture supernatants were collected and assayed using the RayBio human chemokine antibody array 1 and the RayBio human inflammatory marker antibody array 3 (RayBiotech, Norcross, GA, USA). The assays were performed in accordance with the manufacturer's instructions.
Chung K.M., Hsu S.C., Chu Y.R., Lin M.Y., Jiaang W.T., Chen R.H, & Chen X. (2014). Fibroblast Activation Protein (FAP) Is Essential for the Migration of Bone Marrow Mesenchymal Stem Cells through RhoA Activation. PLoS ONE, 9(2), e88772.
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