Glucoamylase from A<.spergillus niger, choline chloride, soluble starch, maltotriose, urea, methyl urea, malonic acid, levulinic acid, ethylene glycol, D-isosorbide, MPh3PBr, glycerol, benzyl alcohol, 1,3-propanediol, glucose, sucrose, α-methyl-glucoside, and methanol were purchased from Sigma Chemical Co. (St. Louis, MO, USA).
Glucoamylase from aspergillus niger
Manufactured by Merck Group
Sourced in United States
Glucoamylase from Aspergillus niger is a laboratory enzyme product. It is an exo-1,4-α-glucosidase that catalyzes the hydrolysis of (1→4)-α-D-glucosidic linkages in polysaccharides, such as starch and glycogen, to release β-D-glucose from the non-reducing ends.
Automatically generated - may contain errors
Lab products found in correlation
Levulinic acid, by Merck Group (1 mentions)
Malonic acid, by Merck Group (1 mentions)
Soluble starch, by Merck Group (1 mentions)
Maltotriose, by Merck Group (1 mentions)
Benzyl alcohol, by Merck Group (1 mentions)
1 3 propanediol, by Merck Group (1 mentions)
α methylglucoside, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Glycerol, by Merck Group (1 mentions)
Sucrose, by Merck Group (1 mentions)
Urea, by Merck Group (1 mentions)
Choline chloride, by Merck Group (1 mentions)
Ethylene glycol, by Merck Group (1 mentions)
Deae cellulose, by Merck Group (1 mentions)
Sephadex g 100, by Merck Group (1 mentions)
Proteinase k recombinant pcr grade, by Roche (1 mentions)
β glucanase, by Novozymes (1 mentions)
Porcine pancreatic α amylase, by Merck Group (1 mentions)
β amylase, by Megazyme (1 mentions)
Isoamylase, by Megazyme (1 mentions)
3 protocols using glucoamylase from aspergillus niger
1
Enzymatic Starch Hydrolysis via Glucoamylase
2
Starch Analysis and Enzyme Characterization
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Substrates and chemicals. DEAE-cellulose, ethylenediamine (EDTA), glucoamylase from Aspergillus niger, Sephadex G-100, p-nitrophenyl (PNP) substrates, termamyl from Bacillus licheniformis and other chemicals were obtained from Sigma Chemical (St. Louis, USA). Resistant starch was procured from HiMaize (Australia). Protein molecular weight markers were purchased from Genie, Bangalore, India. Microbiological media ingredients and culture media were procured from HiMedia, Mumbai, India. All chemicals and solvents used were of analytical grade.
3
Enzyme-Assisted Starch Modification Protocol
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Waxy maize starch (WX) was obtained from Cerestar-AKV I/S (Vodskov, Denmark).
Amylose-only (AO) barley starch was obtained from Aarhus University (Aarhus, Denmark). BE, AM and β-glucanase were kindly provided from Novozymes (Bagsvaerd, Denmark). For BE and AM, one U is defined as 1 µmole/min under standard conditions. Isoamylase (EC 3.2.1.68, specific activity 210 U•mL -1 ) and β-amylase (EC 3.2.1.2, specific activity 620 U•mL -1 ) was obtained from Megazyme (Wicklow, Ireland). Porcine pancreatic α-amylase (EC 3.2.1.1, specific activity 22 U•mg -1 ), and glucoamylase from Aspergillus niger (EC 3.2.1.3, specific activity 129 U•mg -1 ) were purchased from Sigma-Aldrich (Missouri, USA). Proteinase K, recombinant, PCR grade was purchased from Roche (Hvidovre, Denmark). Enzyme activity units of Isoamylase, α-amylase and glucoamylase are given according to the supplier.
Amylose-only (AO) barley starch was obtained from Aarhus University (Aarhus, Denmark). BE, AM and β-glucanase were kindly provided from Novozymes (Bagsvaerd, Denmark). For BE and AM, one U is defined as 1 µmole/min under standard conditions. Isoamylase (EC 3.2.1.68, specific activity 210 U•mL -1 ) and β-amylase (EC 3.2.1.2, specific activity 620 U•mL -1 ) was obtained from Megazyme (Wicklow, Ireland). Porcine pancreatic α-amylase (EC 3.2.1.1, specific activity 22 U•mg -1 ), and glucoamylase from Aspergillus niger (EC 3.2.1.3, specific activity 129 U•mg -1 ) were purchased from Sigma-Aldrich (Missouri, USA). Proteinase K, recombinant, PCR grade was purchased from Roche (Hvidovre, Denmark). Enzyme activity units of Isoamylase, α-amylase and glucoamylase are given according to the supplier.
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